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Recombinant Human HFE His Protein

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SDS-Page: Recombinant Human HFE Protein [NBP2-23110]
SDS-Page: Recombinant Human HFE Protein [NBP2-23110]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

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Recombinant Human HFE His Protein Summary

Description
A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 23-306 of Human HFE

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMRLLRSH SLHYLFMGAS EQDLGLSLFE ALGYVDDQLF VFYDHESRRV EPRTPWVSSR ISSQMWLQLS QSLKGWDHMF TVDFWTIMEN HNHSKESHTL QVILGCEMQE DNSTEGYWKY GYDGQDHLEF CPDTLDWRAA EPRAWPTKLE WERHKIRARQ NRAYLERDCP AQLQQLLELG RGVLDQQVPP LVKVTHHVTS SVTTLRCRAL NYYPQNITMK WLKDKQPMDA KEFEPKDVLP NGDGTYQGWI TLAVPPGEEQ RYTCQVEHPG LDQPLIVIWE PSPSGTLV
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
HFE
Purity
>85%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Application Notes
Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
Theoretical MW
35.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>85%, by SDS-PAGE

Alternate Names for Recombinant Human HFE His Protein

  • dJ221C16.10.1
  • hemochromatosis
  • hereditary hemochromatosis protein HLA-H
  • hereditary hemochromatosis protein
  • HFE
  • HFE1
  • HH
  • high Fe
  • HLAH
  • HLA-H
  • HLA-HHFE1
  • MGC103790
  • MHC class I-like protein HFE
  • MVCD7
  • TFQTL2

Background

HFE, also known as HLA-H, belongs to the MHC class I family. This protein contains 1 Ig-like C1-type (immunoglobulin-like) domain. HFE is a membrane protein that is similar to MHC class I-type proteins and associates with beta-2 microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor (TFR) with transferrin. It binds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin. Recombinant human HFE protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Recombinant Human
HFE His Protein
NBP2-23110
Recombinant Human HFE His Protein
Species: Hu
Applications: PAGE

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Bioinformatics

Gene Symbol HFE