Rat Hexokinase 1 ELISA Kit (Colorimetric) Summary
Specificity |
This kit recognizes Rat Hexokinase 1 in samples. No Significant cross-reactivity or interference between Rat Hexokinase 1 and analogues was observed. |
Standard Curve Range |
31.25 - 2000 pg/mL |
Sensitivity |
56.25 pg/mL |
Assay Type |
Sandwich-ELISA |
Inter-Assay |
CV% < 11% |
Intra-Assay |
CV% < 11% |
Spike Recovery |
average = 95% for serum; average = 97% for EDTA plasma; average = 98% for cell culture media |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
HK1 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab
|
Alternate Names for Rat Hexokinase 1 ELISA Kit (Colorimetric)
Background
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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