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Rat Hexokinase 1 ELISA Kit (Colorimetric)

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ELISA: Rat Hexokinase 1 ELISA Kit (Colorimetric) [NBP2-82213] - Samples were spiked with high concentrations of Rat Hexokinase 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within ...read more
ELISA: Rat Hexokinase 1 ELISA Kit (Colorimetric) [NBP2-82213] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell supernatant
Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
56.25 pg/mL

Order Details

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Rat Hexokinase 1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat Hexokinase 1 in samples. No Significant cross-reactivity or interference between Rat Hexokinase 1 and analogues was observed.
Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
56.25 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 11%
Intra-Assay
CV% < 11%
Spike Recovery
average = 95% for serum; average = 97% for EDTA plasma; average = 98% for cell culture media
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
HK1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Alternate Names for Rat Hexokinase 1 ELISA Kit (Colorimetric)

  • Brain form hexokinase
  • EC 2.7.1
  • EC 2.7.1.1
  • glycolytic enzyme
  • Hexokinase 1
  • Hexokinase type I
  • hexokinase-1
  • HK I
  • HK1
  • HK1-ta
  • HK1-tb
  • HK1-tc
  • HKI
  • HXK1

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol HK1