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Hexokinase 1 Antibody

Images

 
Western Blot: Hexokinase 1 Antibody [NBP1-85789] - Analysis in human cell line PC-3 and human cell line CACO-2.
Immunohistochemistry-Paraffin: Hexokinase 1 Antibody [NBP1-85789] - Staining of human stomach shows strong cytoplasmic positivity in parietal cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Hexokinase 1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVHMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPVGFTFSFPCQQSKIDEAILITWTKRFKASGVEGADVVKLLN
Predicted Species
Mouse (97%), Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:10-1:20
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Hexokinase 1 Protein (NBP1-85789PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-85789 in the following applications:

Publications
Read Publication using NBP1-85789.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Hexokinase 1 Antibody

  • Brain form hexokinase
  • EC 2.7.1
  • EC 2.7.1.1
  • glycolytic enzyme
  • Hexokinase 1
  • Hexokinase type I
  • hexokinase-1
  • HK I
  • HK1
  • HK1-ta
  • HK1-tb
  • HK1-tc
  • HKI
  • HXK1

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IHC

Publications for Hexokinase 1 Antibody (NBP1-85789)(1)

Review for Hexokinase 1 Antibody (NBP1-85789) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-85789:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot Hexokinase 1 NBP1-85789
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4
reviewed by:
Verified Customer
WB Human 08/08/2014
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Summary

ApplicationWestern Blot
Sample TestedNuclear and cytosolic lysates from 293T cells
SpeciesHuman

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Secondary Antibodies

 

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Verified Customer
08/08/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol HK1