![Western Blot: Hexokinase 1 Antibody [NBP1-31600] - Sample (30 ug of whole cell lysate)C: zebrafish muscle7.5% SDS PAGEdiluted at 1:1000](http://images.novusbio.com/fullsize/Hexokinase-1-Antibody-Western-Blot-NBP1-31600-img0010.jpg "Western Blot: Hexokinase 1 Antibody [NBP1-31600] - Sample (30 ug of whole cell lysate)C: zebrafish muscle7.5% SDS PAGEdiluted at 1:1000")
| Reactivity | Hu, Ze, Mu, Rt, PoSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Hexokinase 1. The exact sequence is proprietary. |
| Localization | Mitochondrion outer membrane |
| Predicted Species | Mouse (94%), Rat (92%), Porcine (93%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HK1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC-WM-Assay dependent |
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| Theoretical MW | 102 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris-Glycine (pH7), 10% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
| Publication using NBP1-31600 | Applications | Species |
|---|---|---|
| Rajamanickam GD, Kastelic JP, Thundathil JC Testis-Specific Isoform of Na/K-ATPase (ATP1A4) Interactome in Raft and Non-Raft Membrane Fractions from Capacitated Bovine Sperm Int J Mol Sci 2019-06-28 [PMID: 31261667] (WB, Bovine) | WB | Bovine |
Secondary Antibodies |
Isotype Controls |
Research Areas for Hexokinase 1 Antibody (NBP1-31600)Find related products by research area.
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| Gene Symbol | HK1 |
![Immunocytochemistry/Immunofluorescence: Hexokinase 1 Antibody [NBP1-31600] - Analysis of methanol-fixed HeLa, using HXK I antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/Hexokinase-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31600-img0005.jpg "Immunocytochemistry/Immunofluorescence: Hexokinase 1 Antibody [NBP1-31600] - Analysis of methanol-fixed HeLa, using HXK I antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.")
![Immunohistochemistry Whole-Mount: Hexokinase 1 Antibody [NBP1-31600] - Hexokinase 1 antibody detects Hexokinase 1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo.Hexokinase 1 antibody dilution: 1:100.](http://images.novusbio.com/fullsize/Hexokinase-1-Antibody-Immunohistochemistry-Whole-Mount-NBP1-31600-img0009.jpg "Immunohistochemistry Whole-Mount: Hexokinase 1 Antibody [NBP1-31600] - Hexokinase 1 antibody detects Hexokinase 1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo.Hexokinase 1 antibody dilution: 1:100.")
![Western Blot: Hexokinase 1 Antibody [NBP1-31600] - Sample(30 ug whole cell lysate)A:A431 B:H1299 C:HeLa S3 D:Hep G2 7.5% SDS PAGE, antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/Hexokinase-1-Antibody-Western-Blot-NBP1-31600-img0007.jpg "Western Blot: Hexokinase 1 Antibody [NBP1-31600] - Sample(30 ug whole cell lysate)A:A431 B:H1299 C:HeLa S3 D:Hep G2 7.5% SDS PAGE, antibody diluted at 1:3000.")
![Immunohistochemistry-Paraffin: Hexokinase 1 Antibody [NBP1-31600] - Human breast cancer, using HXK Iantibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Hexokinase-1-Antibody-Immunohistochemistry-Paraffin-NBP1-31600-img0006.jpg "Immunohistochemistry-Paraffin: Hexokinase 1 Antibody [NBP1-31600] - Human breast cancer, using HXK Iantibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: Hexokinase 1 Antibody [NBP1-31600] - Analysis of paraffin-embedded zebrafish tissue, using Hexokinase 1 antibody at 1:300 dilution.](http://images.novusbio.com/fullsize/Hexokinase-1-Antibody-Immunohistochemistry-Paraffin-NBP1-31600-img0008.jpg "Immunohistochemistry-Paraffin: Hexokinase 1 Antibody [NBP1-31600] - Analysis of paraffin-embedded zebrafish tissue, using Hexokinase 1 antibody at 1:300 dilution.")
![Western Blot: Hexokinase 1 Antibody [NBP1-31600] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hexokinase 1 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-31600_rabbit-polyclonal-hexokinase-1-antibody-1610202419405740.jpg "Western Blot: Hexokinase 1 Antibody [NBP1-31600] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hexokinase 1 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")