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Hexokinase 1 Antibody (6B7Q2)

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Western Blot: Hexokinase 1 Antibody (6B7Q2) [NBP3-15274] - Western blot analysis of extracts of various cell lines, using Hexokinase 1 Rabbit mAb (NBP3-15274) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit ...read more
Immunocytochemistry/ Immunofluorescence: Hexokinase 1 Antibody (6B7Q2) [NBP3-15274] - Immunofluorescence analysis of U-2 OS cells using Hexokinase 1 Rabbit mAb (NBP3-15274) at dilution of 1:100 (40x lens). Blue: DAPI ...read more
Immunohistochemistry-Paraffin: Hexokinase 1 Antibody (6B7Q2) [NBP3-15274] - Immunohistochemistry of paraffin-embedded mouse kidney using Hexokinase 1 Rabbit mAb (NBP3-15274) at dilution of 1:100 (40x lens).Perform ...read more
Immunohistochemistry-Paraffin: Hexokinase 1 Antibody (6B7Q2) [NBP3-15274] - Immunohistochemistry of paraffin-embedded rat kidney using Hexokinase 1 Rabbit mAb (NBP3-15274) at dilution of 1:100 (40x lens).Perform ...read more
Immunohistochemistry-Paraffin: Hexokinase 1 Antibody (6B7Q2) [NBP3-15274] - Immunohistochemistry of paraffin-embedded human thyroid cancer using Hexokinase 1 Rabbit mAb (NBP3-15274) at dilution of 1:100 (40x ...read more
Genetic Strategies: Knockout Validated: Hexokinase 1 Antibody (6B7Q2) [NBP3-15274] - Western blot analysis of extracts from normal (control) and Hexokinase 1 knockout (KO) 293T cells, using Hexokinase 1 Rabbit ...read more
Immunoprecipitation: Hexokinase 1 Antibody (6B7Q2) [NBP3-15274] - Immunoprecipitation analysis of 600ug extracts of Mouse brain cells using 3ug Hexokinase 1 antibody (NBP3-15274). Western blot was performed from the ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP, KO
Clone
6B7Q2
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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Hexokinase 1 Antibody (6B7Q2) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Hexokinase 1 (P19367). GLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVHMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPV
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
HK1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:500 - 1:1000
  • Knockout Validated
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Hexokinase 1 Antibody (6B7Q2)

  • Brain form hexokinase
  • EC 2.7.1
  • EC 2.7.1.1
  • glycolytic enzyme
  • Hexokinase 1
  • Hexokinase type I
  • hexokinase-1
  • HK I
  • HK1
  • HK1-ta
  • HK1-tb
  • HK1-tc
  • HKI
  • HXK1

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HK1