![Western Blot: Hemoglobin alpha Antibody (PD00-16) [NBP3-32421] - Western blot analysis of Hemoglobin alpha on rat heart tissue lysates with Rabbit anti-Hemoglobin alpha antibody (NBP3-32421) at 1/2,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 15 kDa Observed band size: 12 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32421) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32421_rabbit-hemoglobin-alpha-mab-pd00-16-278202415242667.jpg "Western Blot: Hemoglobin alpha Antibody (PD00-16) [NBP3-32421] - Western blot analysis of Hemoglobin alpha on rat heart tissue lysates with Rabbit anti-Hemoglobin alpha antibody (NBP3-32421) at 1/2,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 15 kDa Observed band size: 12 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32421) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clone | PD00-16 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Synthetic peptide corresponding to Human Hemoglobin alpha aa 50-150. (Uniprot: P69905) |
| Localization | Cytosol, cytosolic small ribosomal subunit, hemoglobin complex, blood microparticle, extracellular exosome, extracellular region, extracellular space, endocytic vesicle lumen, haptoglobin-hemoglobin complex, membrane. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | HBA1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 15 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.1% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Hemoglobin alpha Antibody (NBP3-32421)Find related products by research area.
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| Gene Symbol | HBA1 |
![Immunohistochemistry: Hemoglobin alpha Antibody (PD00-16) [NBP3-32421] - Immunohistochemical analysis of paraffin-embedded human bone marrow tissue with Rabbit anti-Hemoglobin alpha antibody (NBP3-32421) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody () at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32421_rabbit-hemoglobin-alpha-mab-pd00-16-29820241435248.jpg "Immunohistochemistry: Hemoglobin alpha Antibody (PD00-16) [NBP3-32421] - Immunohistochemical analysis of paraffin-embedded human bone marrow tissue with Rabbit anti-Hemoglobin alpha antibody (NBP3-32421) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody () at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
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