![Western Blot: HADH Antibody [NBP2-16777] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HADH antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HADH-Antibody-Western-Blot-NBP2-16777-img0010.jpg "Western Blot: HADH Antibody [NBP2-16777] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HADH antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HADH. The exact sequence is proprietary. |
| Localization | Mitochondrion matrix |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HADH |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 34 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for HADH Antibody (NBP2-16777)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: HADH Antibody [NBP2-16777] - HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37C for 30 min. Green: HADH protein stained by HADH antibody diluted at 1:500. Blue: Hoechst 33343 staining.](http://images.novusbio.com/fullsize/HADH-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16777-img0005.jpg "Immunocytochemistry/Immunofluorescence: HADH Antibody [NBP2-16777] - HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37C for 30 min. Green: HADH protein stained by HADH antibody diluted at 1:500. Blue: Hoechst 33343 staining.")
![Immunohistochemistry-Paraffin: HADH Antibody [NBP2-16777] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/HADH-Antibody-Immunohistochemistry-Paraffin-NBP2-16777-img0006.jpg "Immunohistochemistry-Paraffin: HADH Antibody [NBP2-16777] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.")
![Western Blot: HADH Antibody [NBP2-16777] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE](http://images.novusbio.com/fullsize/HADH-Antibody-Western-Blot-NBP2-16777-img0007.jpg "Western Blot: HADH Antibody [NBP2-16777] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE")
![Western Blot: HADH Antibody [NBP2-16777] - A: Mouse Liver.](http://images.novusbio.com/fullsize/HADH-Antibody-Western-Blot-NBP2-16777-img0008.jpg "Western Blot: HADH Antibody [NBP2-16777] - A: Mouse Liver.")
![Western Blot: HADH Antibody [NBP2-16777] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HADH antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HADH-Antibody-Western-Blot-NBP2-16777-img0009.jpg "Western Blot: HADH Antibody [NBP2-16777] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HADH antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")