![Western Blot: H4/n Antibody (7A2A7) [NBP2-52488] - Analysis using HIST2H4A(20Me3) mouse mAb against THP-1 (1), Jurkat (2), K562 (3), NIT/3T3 (4), PC-12 (5), Hela (6), MCF-7 (7), and A431 (8) cell lysate.](http://images.novusbio.com/fullsize/H4-n-Antibody-7A2A7-Western-Blot-NBP2-52488-img0003.jpg "Western Blot: H4/n Antibody (7A2A7) [NBP2-52488] - Analysis using HIST2H4A(20Me3) mouse mAb against THP-1 (1), Jurkat (2), K562 (3), NIT/3T3 (4), PC-12 (5), Hela (6), MCF-7 (7), and A431 (8) cell lysate.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, Flow, CyTOF-ready |
| Clone | 7A2A7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Synthesized peptide of human H4/n (AA: GGAKRHRK(Me3)VLRDNIQ) . |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | HIST2H4A |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is Cytof ready. |
| Theoretical MW | 11.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | HIST2H4A |
![Flow Cytometry: H4/n Antibody (7A2A7) [NBP2-52488] - Analysis of Raji cells using HIST2H4A(20Me3) mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/H4-n-Antibody-7A2A7-Flow-Cytometry-NBP2-52488-img0001.jpg "Flow Cytometry: H4/n Antibody (7A2A7) [NBP2-52488] - Analysis of Raji cells using HIST2H4A(20Me3) mouse mAb (green) and negative control (red).")
![ELISA: H4/n Antibody (7A2A7) [NBP2-52488] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/H4-n-Antibody-7A2A7-ELISA-NBP2-52488-img0002.jpg "ELISA: H4/n Antibody (7A2A7) [NBP2-52488] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).")
![Western Blot CBP/KAT3A Antibody (257003) [Unconjugated]](https://images.novusbio.com/images2/CBP_MAB2676_Western_Blot_6043.jpg)
![Immunocytochemistry CBP/KAT3A Antibody (257003) [Unconjugated]](https://images.novusbio.com/images2/mab2676_human-mouse-rat-cbp-mab-clone-257003-immunocytochemistry-101220208237.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")