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GSK-3 beta Antibody (272536) [Unconjugated]

 (7 Publications)
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Western blot shows lysates of HT-29 human colon adenocarcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL Human/Mouse/Rat GSK-3 beta Monoclonal Antibody (Catalog # MAB2506) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant human GSK-3a and Recombinant Human Active GSK-3 beta (Catalog # 2506-KS) (20 ng/lane) were included. A specific band for GSK-3 beta was detected at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HT-29 human colon adenocarcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL Human/Mouse/Rat GSK-3 beta Monoclonal Antibody ...read more
GSK-3 beta was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Rat Anti-Human/Mouse/Rat GSK-3 beta Monoclonal Antibody (Catalog # MAB2506) at 3 µg/mL for 3 hours at room temperature. ...read more
GSK-3 beta was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Rat Anti-Human/Mouse/Rat GSK-3 beta Monoclonal Antibody (Catalog # MAB2506) at 3 µg/mL for 3 hours at room temperature. ...read more
BSSM activates AKT/GSK3 beta pathway, promotes tau hyperphosphorylation and PHF accumulation and increases the expression of active asparagine endopeptidase (AEP) and tau cleavage. (A,B) Western blotting showed higher ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, CyTOF-ready, Flow, ICC/IF
Clone
272536
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC2506U-100UG
Alexa Fluor 405
IC2506V-100UG
Alexa Fluor 488
IC2506G-100UG
Alexa Fluor 532
FAB2506X
Alexa Fluor 594
IC2506T-100UG
Alexa Fluor 647
IC2506R-100UG
Alexa Fluor 700
IC2506N-100UG
Alexa Fluor 750
IC2506S-100UG
Allophycocyanin
FAB2506A
Biotin
FAB2506B
CoraFluor 1
FAB2506CL1
DyLight 350
FAB2506D
DyLight 405
FAB2506E
DyLight 488
FAB2506K
DyLight 550
FAB2506L
DyLight 594
FAB2506M
DyLight 650
FAB2506W
DyLight 680
FAB2506Y
DyLight 755
FAB2506Z
Fluorescein
IC2506F
HRP
FAB2506H
Janelia Fluor 525
FAB2506JF525
Janelia Fluor 549
FAB2506I
Janelia Fluor 646
FAB2506J
Janelia Fluor 669
FAB2506JF669
PE
FAB2506P
PerCP
FAB2506C
mFluor Violet 450 SE
FAB2506MFV450
mFluor Violet 500 SE
FAB2506MFV500
mFluor Violet 610 SE
FAB2506MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
IC2506F
Carrier Free
MAB2506-SP
Carrier Free
IC2506V-100UG
Carrier Free
IC2506U-100UG
Carrier Free
IC2506T-100UG
Carrier Free
IC2506S-100UG
Carrier Free
IC2506R-100UG
Carrier Free
IC2506N-100UG
Carrier Free
IC2506G-100UG
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GSK-3 beta Antibody (272536) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GSK-3 beta
Met1-Thr420
Accession # P49841
Specificity
Detects human, mouse, and rat GSK-3 beta  in ELISAs and Western blots. Does not cross-react with recombinant human GSK-3 alpha .
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
GSK3B
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 3-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB2506 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GSK-3 beta Antibody (272536) [Unconjugated]

  • EC 2.7.11
  • EC 2.7.11.26
  • glycogen synthase kinase 3 beta
  • glycogen synthase kinase-3 beta
  • GSK3 beta
  • GSK-3 beta
  • GSK3B
  • GSK3beta isoform

Background

Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta ) share 85% amino acid identity. GSK-3 beta , inhibited by phosphorylation at S9 by Akt, is involved in energy metabolism, body pattern formation, and neuronal cell development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GSK-3 beta Antibody (MAB2506)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: Flow Cytometry, Western Blot.


Filter By Application
Flow Cytometry
(1)
Western Blot
(3)
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Human
(3)
Mouse
(1)
Rat
(1)
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Showing Publications 1 - 7 of 7.
Publications using MAB2506 Applications Species
Wolf, B;Muralidharan, P;Lee, MY;Hua, W;Green, E;Wang, H;Strange, C; Overexpression of Alpha-1 Antitrypsin Increases the Proliferation of Mesenchymal Stem Cells by Upregulation of Cyclin D1 International journal of molecular sciences 2024-02-07 [PMID: 38396691] (Western Blot, Human) Western Blot Human
Kennedy, KE;Kerlero de Rosbo, N;Uccelli, A;Cellerino, M;Ivaldi, F;Contini, P;De Palma, R;Harbo, HF;Berge, T;Bos, SD;Høgestøl, EA;Brune-Ingebretsen, S;de Rodez Benavent, SA;Paul, F;Brandt, AU;Bäcker-Koduah, P;Behrens, J;Kuchling, J;Asseyer, S;Scheel, M;Chien, C;Zimmermann, H;Motamedi, S;Kauer-Bonin, J;Saez-Rodriguez, J;Rinas, M;Alexopoulos, LG;Andorra, M;Llufriu, S;Saiz, A;Blanco, Y;Martinez-Heras, E;Solana, E;Pulido-Valdeolivas, I;Martinez-Lapiscina, EH;Garcia-Ojalvo, J;Villoslada, P; Multiscale networks in multiple sclerosis PLoS computational biology 2024-02-01 [PMID: 38329927] (Flow Cytometry, Human) Flow Cytometry Human
Wolf, B;Muralidharan, P;Lee, M;Hua, W;Green, E;Wang, H;Strange, C; Overexpression of Alpha-1 Antitrypsin Increases the Proliferation of Mesenchymal Stem Cells by Upregulation of Cyclin D1 and is Independent of the Wnt Signaling Pathway bioRxiv : the preprint server for biology 2023-10-28 [PMID: 37961658] (Western Blot, Human) Western Blot Human
Gang Liu, Fengwei Yin, Congli Zhang, Yang Zhang, Xiaohong Li, Yunzhi Ling Effects of regulating miR-132 mediated GSK-3 beta on learning and memory function in mice Experimental and Therapeutic Medicine 2020-05-19 [PMID: 32742357]
Shuke Nie, Yang Tan, Zhentao Zhang, Guiqin Chen, Jing Xiong, Dan Hu et al. Bilateral Implantation of Shear Stress Modifier in ApoE Knockout Mouse Induces Cognitive Impairment and Tau Abnormalities Frontiers in Aging Neuroscience 2018-10-04 [PMID: 30337867]
Yang Tan 7,8-Dihydroxyflavone Ameliorates Cognitive Impairment by Inhibiting Expression of Tau Pathology in ApoE-Knockout Mice Front Aging Neurosci, 2016-11-29;8(0):287. 2016-11-29 [PMID: 27965573] (Mouse) Mouse
Ye P, Hu Q, Liu H, Yan Y, D'ercole AJ beta-catenin mediates insulin-like growth factor-I actions to promote cyclin D1 mRNA expression, cell proliferation and survival in oligodendroglial cultures. Glia, 2010-07-01;58(9):1031-41. 2010-07-01 [PMID: 20235220] (Western Blot, Rat) Western Blot Rat

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Bioinformatics

Gene Symbol GSK3B
Uniprot