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GSK-3 beta Antibody (272536) [Fluorescein]

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HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human/Mouse/Rat GSK-3 beta Fluorescein-conjugated Monoclonal Antibody (Catalog # IC2506F, filled histogram) or isotype control antibody (Catalog # IC006F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human/Mouse/Rat GSK-3 beta Fluorescein-conjugated Monoclonal Antibody (Catalog # IC2506F, filled histogram) or isotype control antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Flow
Clone
272536
Clonality
Monoclonal
Host
Rat
Conjugate
Fluorescein

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC2506U-100UG
Alexa Fluor 405
IC2506V-100UG
Alexa Fluor 488
IC2506G-100UG
Alexa Fluor 532
FAB2506X
Alexa Fluor 594
IC2506T-100UG
Alexa Fluor 647
IC2506R-100UG
Alexa Fluor 700
IC2506N-100UG
Alexa Fluor 750
IC2506S-100UG
Allophycocyanin
FAB2506A
Biotin
FAB2506B
CoraFluor 1
FAB2506CL1
DyLight 350
FAB2506D
DyLight 405
FAB2506E
DyLight 488
FAB2506K
DyLight 550
FAB2506L
DyLight 594
FAB2506M
DyLight 650
FAB2506W
DyLight 680
FAB2506Y
DyLight 755
FAB2506Z
HRP
FAB2506H
Janelia Fluor 525
FAB2506JF525
Janelia Fluor 549
FAB2506I
Janelia Fluor 646
FAB2506J
Janelia Fluor 669
FAB2506JF669
PE
FAB2506P
PerCP
FAB2506C
Unconjugated
MAB2506
mFluor Violet 450 SE
FAB2506MFV450
mFluor Violet 500 SE
FAB2506MFV500
mFluor Violet 610 SE
FAB2506MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB2506
Carrier Free
MAB2506
Carrier Free
MAB2506-SP
Carrier Free
IC2506V-100UG
Carrier Free
IC2506U-100UG
Carrier Free
IC2506T-100UG
Carrier Free
IC2506S-100UG
Carrier Free
IC2506R-100UG
Carrier Free
IC2506N-100UG
Carrier Free
IC2506G-100UG
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GSK-3 beta Antibody (272536) [Fluorescein] Summary

Immunogen
E. coli-derived recombinant human GSK-3 beta
Met1-Thr420
Accession # P49841
Specificity
Detects human, mouse, and rat GSK-3 beta  in ELISAs and Western blots. Does not cross-react with recombinant human GSK-3 alpha .
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
GSK3B
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GSK-3 beta Antibody (272536) [Fluorescein]

  • EC 2.7.11
  • EC 2.7.11.26
  • glycogen synthase kinase 3 beta
  • glycogen synthase kinase-3 beta
  • GSK3 beta
  • GSK-3 beta
  • GSK3B
  • GSK3beta isoform

Background

Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta ) share 85% amino acid sequence identity. GSK-3 beta , inhibited by phosphorylation at S9 by Akt, is involved in energy metabolism, body pattern formation, and neuronal cell development. Depending upon the substrate, phosphorylation may either promote activity (SIK-1; OGT; ZC3HAV1) or downregulate activity (SNAI1; BMAL1; MARK2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GSK3B
Uniprot