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GM130/GOLGA2 Antibody [Unconjugated]

Images

 
Western blot shows lysates of MCF-7 human breast cancer cell line and ZR-75 human breast cancer cell line. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human GM130/GOLGA2 Antigen Affinity-purified Polyclonal ...read more
GM130/GOLGA2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human GM130/GOLGA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8199) at 1.7 µg/mL for 3 ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, and ZR‑75 human breast cancer cell line, loaded at 0.2 mg/mL. A specific ...read more
Kcnj13 orchestrates SM cell alignment and polarity. a Immunostaining for alpha SMA (red) and DAPI staining (blue) in dorsal views of E14.5 WT (n = 8) and Kcnj13T38C/T38C (n = 8) tracheas. b Quantification of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
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GM130/GOLGA2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GM130/GOLGA2
Met528-Gln606
Accession # Q08379
Specificity
Detects human GM130/GOLGA2 in direct ELISAs and Western blots.   
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
GOLGA2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Simple Western 50 ug/mL
  • Western Blot 0.25 ug/mL
Reviewed Applications
Read 1 Review rated 3
using
AF8199 in the following applications:

Publications
Read Publications using
AF8199 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GM130/GOLGA2 Antibody [Unconjugated]

  • 130 kDa cis-Golgi matrix protein
  • GM130 autoantigen
  • GM130
  • GM130Golgi matrix protein GM130
  • GOLGA2
  • golgi autoantigen, golgin subfamily a, 2
  • golgin A2
  • Golgin subfamily A member 2
  • Golgin-95
  • MGC20672
  • SY11 Protein

Background

GM130, also known as Golgin subfamily A member 2 and GOLGA2, is a 130 kDa member of the Golgin protein family.  GM130/GOLGA2 is a component of the Golgi membrane involved in maintaining cis-Golgi structure.  GM130/GOLGA2 protein is thought to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been predicted. Human GM130/GOLGA2 protein is 1002 amino acids (aa) in length, and over aa 528‑606, shares 90% sequence identity with its mouse and rat orthologs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GM130/GOLGA2 Antibody (AF8199)(13)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC, IHC, Western Blot.


Filter By Application
ICC
(4)
IHC
(2)
Western Blot
(1)
All Applications
Filter By Species
Human
(5)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF8199 Applications Species
Haoyu Wu, Ping Wang, Ziying Liu, Chunyan Lu, Wenguang Yin Systematic Analysis of Smooth Muscle and Cartilage Ring Formation during Mouse Tracheal Tubulogenesis BIO-PROTOCOL 7/5/2023 [PMID: 37449041]
Zinaida A. Dubeykovskaya, Nguyen Huu Tu, Paulina D. Ramírez Garcia, Brian L. Schmidt, Donna G. Albertson Oral Cancer Cells Release Vesicles that Cause Pain Advanced Biology 9/1/2022 [PMID: 35802912]
Nicole Schäfer, Anas Rasras, Delia M. Ormenisan, Sabine Amslinger, Volker Enzmann, Herbert Jägle et al. Complement Factor H-Related 3 Enhanced Inflammation and Complement Activation in Human RPE Cells Frontiers in Immunology 11/8/2021 [PMID: 34819935]
Jennifer Y. Liu, Yu-Hsiu Tony Lin, Andrew M. Leidal, Hector H. Huang, Jordan Ye, Arun P. Wiita et al. GRASP55 restricts early-stage autophagy and regulates spatial organization of the early secretory network Biology Open 10/15/2021 [PMID: 34533192]
K Chaumonnot, S Masson, H Sikner, A Bouchard, V Baverel, PS Bellaye, B Collin, C Garrido, E Kohli The HSP GRP94 interacts with macrophage intracellular complement C3 and impacts M2 profile during ER stress Cell Death & Disease, 2021-01-22;12(1):114. 2021-01-22 [PMID: 33483465] (ICC, Human) ICC Human
A Bhattachar, MN Janal, R Veeramacha, I Dolgalev, Z Dubeykovsk, NH Tu, H Kim, S Zhang, AK Wu, M Hagiwara, AR Kerr, MD DeLacure, BL Schmidt, DG Albertson Oncogenes overexpressed in metastatic oral cancers from patients with pain: potential pain mediators released in exosomes Sci Rep, 2020-09-07;10(1):14724. 2020-09-07 [PMID: 32895418] (Western Blot, Human) Western Blot Human
Y Liu, H Wang, J Zhang, J Yang, L Bai, B Zheng, T Zheng, Y Wang, J Li, W Zhang SERINC5 Inhibits the Secretion of Complete and Genome-Free Hepatitis B Virions Through Interfering With the Glycosylation of the HBV Envelope Front Microbiol, 2020-04-30;11(0):697. 2020-04-30 [PMID: 32431673] (ICC, Human) ICC Human
AM Lopez-Guer, N Espinosa-B, I Sanchez-Lo, T Macartney, C Pascual-Ca, Y Orantos-Ag, L Rodriguez-, AB Perez-Oliv, V Mulero, E Pozo-Guisa, FJ Martin-Rom RAC1-Dependent ORAI1 Translocation to the Leading Edge Supports Lamellipodia Formation and Directional Persistence Sci Rep, 2020-04-20;10(1):6580. 2020-04-20 [PMID: 32313105] (ICC, Human) ICC Human
Mary S. Dickinson, Lindsey N. Anderson, Bobbie-Jo M. Webb-Robertson, Joshua R. Hansen, Richard D. Smith, Aaron T. Wright et al. Proximity-dependent proteomics of the Chlamydia trachomatis inclusion membrane reveals functional interactions with endoplasmic reticulum exit sites PLOS Pathogens 4/3/2019 [PMID: 30943267]
Wenguang Yin, Hyun-Taek Kim, ShengPeng Wang, Felix Gunawan, Rui Li, Carmen Buettner et al. Fibrillin-2 is a key mediator of smooth muscle extracellular matrix homeostasis during mouse tracheal tubulogenesis European Respiratory Journal 3/1/2019 [PMID: 30578393]
Show All 13 Publications.

Review for GM130/GOLGA2 Antibody (AF8199) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF8199:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry GM130/GOLGA2 AF8199
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3
reviewed by:
Verified Customer
ICC Human 11/08/2020
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Summary

ApplicationImmunocytochemistry
Sample TestedHuman primary fibroblast
SpeciesHuman
LotCIEQ0117051

Comments

CommentsGood antbody to label Golgi in multiple ICC because it is produced in Sheep. It comes lyophilized, and after reconstitution and saved at -20ºC it precipitates in 6 month, becoming useless. ICC conditions: PFA 4% 20 min fixed cells. Blocking 10% DS in PBST (0.1% Triton) 1h RT. Primary ab O/N incubation 4ºC 5 ug/mL in DS 10% in PBST (0.1% Triton). Secondary Donkey anti-Sheep Alexa fluor 488 1:500 1h RT.

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Recent Reviews

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Verified Customer
11/08/2020
Application: ICC
Species: Human

Bioinformatics

Gene Symbol GOLGA2
Uniprot