| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-53420SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF8199-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB8199-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2622-0.025mg | |||
![Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP2-53420 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-53420-img0007.jpg "Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP2-53420 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-GM130/GOLGA2 Antibody NBP2-53420 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-53420-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-GM130/GOLGA2 Antibody NBP2-53420 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-53420-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - HeLa cells were fixed for 10 min using 10% formalin and then permeabilized for 5 min using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-GOLGA2 at 1:200 overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-53420-img0001.jpg)
![Western Blot: GM130/GOLGA2 Antibody [NBP2-53420] - Total protein from human HeLa and mouse Neuro2A cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/mL anti-GOLGA2 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Western-Blot-NBP2-53420-img0002.jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP2-53420] - Analysis of a FFPE tissue section of mouse intesting using GM130/GOLGA2 antibody at 1:1500. The antibody generated staining in the the Golgi apparatus in the perinuclear region.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP2-53420-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with GM130/GOLGA2 Antibody conjugated to Biotin (NBP2-53420B) at 5 ug/ml for 1 hour at room temperature and detected with Streptavidin conjugated to DyLight 488. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-53420-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with GM130/GOLGA2 Antibody conjugated to FITC (NBP2-53420F) at 2 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-53420-img0015.jpg)
![Flow Cytometry: GM130/GOLGA2 Antibody [NBP2-53420] - An intracellular stain was performed on NIH3T3 cells with NBP2-53420 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Flow-Cytometry-NBP2-53420-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with GM130/GOLGA2 Antibody conjugated to FITC (NBP2-53420F) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-53420-img0014.jpg)
![Flow Cytometry: GM130/GOLGA2 Antibody [NBP2-53420] - An intracellular stain was performed on HeLa cells with NBP2-53420AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 min at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Flow-Cytometry-NBP2-53420-img0004.jpg)
![Flow Cytometry: GM130/GOLGA2 Antibody [NBP2-53420] - An intracellular stain was performed on MCF7 cells with GM130/GOLGA2 Antibody NBP2-53420AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Flow-Cytometry-NBP2-53420-img0005.jpg)
![Flow Cytometry: GM130/GOLGA2 Antibody [NBP2-53420] - An intracellular stain was performed on HeLa cells with NBP2-53420 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Flow-Cytometry-NBP2-53420-img0011.jpg)
![Immunohistochemistry: Rabbit Polyclonal GM130/GOLGA2 Antibody [NBP2-53420] - Staining of mouse C57BL/6 retina using GM130/GOLGA2 Antibody. GOLGA (red), BS-1 lectin (green), DAPI (blue). Primary antibody dilution: 1:200. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-53420_rabbit-polyclonal-gm130-golga2-antibody-immunohistochemistry-165202491154..jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89756] - Staining of human cerebral cortex, colon, lymph node and testis using Anti-GOLGA2 antibody NBP1-89756 (A) shows similar protein distribution across tissues to independent antibody NBP1-89758 (B).](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89756-img0013.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89756] - Staining of human cerebral cortex, colon, lymph node and testis using Anti-GOLGA2 antibody NBP1-89756 (A) shows similar protein distribution across tissues to independent antibody NBP1-89758 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: GM130/GOLGA2 Antibody [NBP1-89756] - Analysis using Anti-GOLGA2 antibody NBP1-89756 (A) shows similar pattern to independent antibody NBP1-89758 (B).](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Western-Blot-NBP1-89756-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP1-89756] - Staining of human cell line U-251 MG shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89756-img0012.jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89756] - Staining of human lymph node using Anti-GOLGA2 antibody NBP1-89756.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89756-img0018.jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89756] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89756-img0007.jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89756] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89756-img0008.jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89756] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89756-img0015.jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89756] - Staining of human testis.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89756-img0016.jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89756] - Staining of human colon.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89756-img0017.jpg)
![Western Blot: GM130/GOLGA2 Antibody [NBP1-89757] - Analysis using Anti-GOLGA2 antibody NBP1-89757 (A) shows similar pattern to independent antibody NBP1-89756 (B).](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Western-Blot-NBP1-89757-img0017.jpg "<b>Independent Antibodies Validation. </b>Western Blot: GM130/GOLGA2 Antibody [NBP1-89757] - Analysis using Anti-GOLGA2 antibody NBP1-89757 (A) shows similar pattern to independent antibody NBP1-89756 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP1-89757] - Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89757-img0012.jpg)
![Immunohistochemistry: GM130/GOLGA2 Antibody [NBP1-89757] - Immunohistochemical staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-NBP1-89757-img0023.jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89757] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89757-img0014.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89757] - Staining in human parathyroid gland and lymph node tissues using anti-GOLGA2 antibody. Corresponding GOLGA2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89757-img0015.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89757] - Staining of human cerebral cortex, colon, lymph node and testis using Anti-GOLGA2 antibody NBP1-89757 (A) shows similar protein distribution across tissues to independent antibody NBP1-89756 (B).](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89757-img0016.jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89757] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89757-img0018.jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89757] - Staining of human testis.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89757-img0019.jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89757] - Staining of human colon.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89757-img0020.jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89757] - Staining of human lymph node using Anti-GOLGA2 antibody NBP1-89757.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89757-img0022.jpg)
![Western Blot: GM130/GOLGA2 Antibody (2059D) [MAB8199] - Analysis of ZR-75 cell lysate with 0.5 ug/ml of hGM130 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hGM130 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-2059D-Western-Blot-MAB8199-img0001.jpg "Western Blot: GM130/GOLGA2 Antibody (2059D) [MAB8199] - Analysis of ZR-75 cell lysate with 0.5 ug/ml of hGM130 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hGM130 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.")
![Western Blot: GM130/GOLGA2 Antibody [H00002801-B01P] - Analysis of GOLGA2 expression in human stomach.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Western-Blot-H00002801-B01P-img0007.jpg "Western Blot: GM130/GOLGA2 Antibody [H00002801-B01P] - Analysis of GOLGA2 expression in human stomach.")
![Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [H00002801-B01P] - Rab1A (blue) is localised primarily on the cis-Golgi, labelled by the GM130 marker (red). Image collected and cropped by CiteAb from the following publication (nature.com/articles/onc2015363), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunocytochemistry-Immunofluorescence-H00002801-B01P-img0009.jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [H00002801-B01P] - Analysis of purified antibody to GOLGA2 on formalin-fixed paraffin-embedded human esophagus. (antibody concentration 3 ug/ml)](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-H00002801-B01P-img0006.jpg)
![Western Blot: GM130/GOLGA2 Antibody [H00002801-B01P] - Analysis of GOLGA2 expression in transfected 293T cell line by GOLGA2 polyclonal antibody. Lane 1: GOLGA2 transfected lysate(37.95 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Western-Blot-H00002801-B01P-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [H00002801-B01P] - Analysis of purified antibody to GOLGA2 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunocytochemistry-Immunofluorescence-H00002801-B01P-img0005.jpg)
![Western Blot: GM130/GOLGA2 Antibody [NBP1-89758] - Analysis using Anti-GOLGA2 antibody NBP1-89758 (A) shows similar pattern to independent antibody NBP1-89756 (B).](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Western-Blot-NBP1-89758-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: GM130/GOLGA2 Antibody [NBP1-89758] - Analysis using Anti-GOLGA2 antibody NBP1-89758 (A) shows similar pattern to independent antibody NBP1-89756 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89758] - Staining of human lymph node using Anti-GOLGA2 antibody NBP1-89758.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89758-img0014.jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89758] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89758-img0006.jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89758] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89758-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89758] - Staining in human parathyroid gland and lymph node tissues using anti-GOLGA2 antibody. Corresponding GOLGA2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89758-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89758] - Staining of human cerebral cortex, colon, lymph node and testis using Anti-GOLGA2 antibody NBP1-89758 (A) shows similar protein distribution across tissues to independent antibody NBP1-89756 (B).](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89758-img0009.jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89758] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89758-img0011.jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89758] - Staining of human testis.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89758-img0012.jpg)
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89758] - Staining of human colon.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89758-img0013.jpg)
![Immunohistochemistry: GM130/GOLGA2 Antibody (SR1125) [NBP3-21627] - Immunohistochemical analysis of paraffin-embedded human cervix carcinoma, using NBP3-21627.](http://images.novusbio.com/fullsize/nbp3-21627_rabbit-gm130-golga2-mab-sr1125-6720239533725.jpg "Immunohistochemistry: GM130/GOLGA2 Antibody (SR1125) [NBP3-21627] - Immunohistochemical analysis of paraffin-embedded human cervix carcinoma, using NBP3-21627.")
![Western Blot: GM130/GOLGA2 Antibody (SR1125) [NBP3-21627] - Western blot analysis using NBP3-21627 in (1) HeLa cell lysate; (2) MCF-7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21627_rabbit-gm130-golga2-mab-sr1125-5720231754852.jpg)
![Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody (M342) [NBP3-23353] - Immunocytochemical labeling of GM130/GOLGA2 in C2C12 cells. The cells were double-labeled with GM130/GOLGA2 (golgi protein) mouse monoclonal and nSMase-3 rabbit polyclonal antibodies, then detected using appropriate secondary antibody conjugated to Cy2 or Cy3.](http://images.novusbio.com/fullsize/nbp3-23353_mouse-gm130-golga2-mab-m342-19920231240370.jpg "Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody (M342) [NBP3-23353] - Immunocytochemical labeling of GM130/GOLGA2 in C2C12 cells. The cells were double-labeled with GM130/GOLGA2 (golgi protein) mouse monoclonal and nSMase-3 rabbit polyclonal antibodies, then detected using appropriate secondary antibody conjugated to Cy2 or Cy3.")
![Western Blot: GM130/GOLGA2 Antibody (M342) [NBP3-23353] - Western blot analysis of GM130/GOLGA2 expression in human cells: MCF7 breast carcinoma (lane 1), A549 adenocarcinoma (lane 2), and MDA-MB-231 breast carcinoma (lane 3). The blot was probed with mouse monoclonal anti-GM130/GOLGA2 (C-terminal region) at 1:500.](http://images.novusbio.com/fullsize/nbp3-23353_mouse-gm130-golga2-mab-m342-1192023166539.jpg)
![Western Blot: GM130/GOLGA2 Antibody [H00002801-B02P] - Analysis of GOLGA2 expression in human placenta.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Western-Blot-H00002801-B02P-img0002.jpg "Western Blot: GM130/GOLGA2 Antibody [H00002801-B02P] - Analysis of GOLGA2 expression in human placenta.")
![Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [H00002801-B02P] - Analysis of purified antibody to GOLGA2 on HepG2 cell. (antibody concentration 7 ug/ml)](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunocytochemistry-Immunofluorescence-H00002801-B02P-img0001.jpg)
![Western Blot: GM130/GOLGA2 Antibody [H00002801-B02P] - Analysis of GOLGA2 expression in transfected 293T cell line by GOLGA2 polyclonal antibody. Lane1:GOLGA2 transfected lysate(108.9 KDa). Lane2:Non-transfected lysate.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Western-Blot-H00002801-B02P-img0003.jpg)
![Western Blot: GM130/GOLGA2 Antibody (JE42-53) [NBP3-32394] - Western blot analysis of GM130/GOLGA2 on MCF-7 cell lysates with Rabbit anti-GM130/GOLGA2 antibody (NBP3-32394) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 113 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32394) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32394_rabbit-gm130-golga2-mab-je42-53-27820241631623.jpg "Western Blot: GM130/GOLGA2 Antibody (JE42-53) [NBP3-32394] - Western blot analysis of GM130/GOLGA2 on MCF-7 cell lysates with Rabbit anti-GM130/GOLGA2 antibody (NBP3-32394) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 113 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32394) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: GM130/GOLGA2 Antibody (JE42-53) [NBP3-32394] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-GM130/GOLGA2 antibody (NBP3-32394) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32394) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32394_rabbit-gm130-golga2-mab-je42-53-298202414262685.jpg)