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GM130/GOLGA2 Antibody (NN 2C10/1) [CoraFluor™ 1]

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, IHC, IP
Clone
NN 2C10/1
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-2622AF350
Alexa Fluor 405
NB100-2622AF405
Alexa Fluor 488
NB100-2622AF488
Alexa Fluor 532
NB100-2622AF532
Alexa Fluor 594
NB100-2622AF594
Alexa Fluor 647
NB100-2622AF647
Alexa Fluor 700
NB100-2622AF700
Alexa Fluor 750
NB100-2622AF750
Biotin
NB100-2622B
DyLight 350
NB100-2622UV
DyLight 405
NB100-2622V
DyLight 488
NB100-2622G
DyLight 550
NB100-2622R
DyLight 594
NB100-2622DL594
DyLight 650
NB100-2622C
DyLight 680
NB100-2622FR
DyLight 755
NB100-2622IR
FITC
NB100-2622F
HRP
NB100-2622H
Janelia Fluor 525
NB100-2622JF525
Janelia Fluor 549
NB100-2622JF549
Janelia Fluor 646
NB100-2622JF646
Janelia Fluor 669
NB100-2622JF669
Unconjugated
NB100-2622
mFluor Violet 450 SE
NB100-2622MFV450
mFluor Violet 500 SE
NB100-2622MFV500
mFluor Violet 610 SE
NB100-2622MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-2622
BSA Free
NB100-2622
BSA Free
NB100-2622-0.025mg
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GM130/GOLGA2 Antibody (NN 2C10/1) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Recombinant purified rat GM130/GOLGA2
Localization
Peripheral membrane protein associated with Golgi stacks
Marker
Golgi Apparatus Marker
Specificity
Does not cross react with human protein.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GOLGA2
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for GM130/GOLGA2 Antibody (NN 2C10/1) [CoraFluor™ 1]

  • 130 kDa cis-Golgi matrix protein
  • GM130 autoantigen
  • GM130
  • GM130Golgi matrix protein GM130
  • GOLGA2
  • golgi autoantigen, golgin subfamily a, 2
  • golgin A2
  • Golgin subfamily A member 2
  • Golgin-95
  • MGC20672
  • SY11 Protein

Background

Golgi Matrix Protein (GM130) is peripheral cytoplamic protein that is bound to the Golgi membranes. It has been implicated as a maintainer of cis-Golgi structure (1). During mitosis, it regulates the disassembly and reassembly of the Golgi complex. It is also linked to docking and fusion of coatomer (COP I) coated vesicles to the Golgi membrane (2). The COOH-terminal domain of GM130 is highly homologous to Golgi human auto-antigen, golgin-95. GM130 interacts specifically and GTP-dependent manner with Rab1b protein, a regulator of anterograde traffic between ER and Golgi membranes (3). GM130 is also activator of YSK1, an essential player in cell migration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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GM130/GOLGA2
Antibody (NN 2C10/1) ...
NB100-2622CL1
GM130/GOLGA2 Antibody (NN 2C10/1) [CoraFluor™ 1]
Species: Rt
Applications: WB, IHC, IP

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Bioinformatics

Gene Symbol GOLGA2