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Golgin 97 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Golgin 97 Antibody [NBP2-38528] - Analysis in human testis and skeletal muscle tissues. Corresponding GOLGA1 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Golgin 97 Antibody [NBP2-38528] - Staining of human cell line A549 shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Golgin 97 Antibody [NBP2-38528] - Staining of human tonsil shows moderate cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: Golgin 97 Antibody [NBP2-38528] - Staining of human colon shows strong positivity in Golgi apparatus in glandular cells.
Immunohistochemistry-Paraffin: Golgin 97 Antibody [NBP2-38528] - Staining of human skeletal muscle shows very weak positivity in myocytes.
Immunohistochemistry-Paraffin: Golgin 97 Antibody [NBP2-38528] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Western Blot: Golgin 97 Antibody [NBP2-38528] -Analysis in human cell line TD47D.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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Golgin 97 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: TGAESKITALEQKEQELQALIQQLSIDLQKVTAETQEKEDVITHLQEKVASLEKRLEQNLSGEEHLQELLK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GOLGA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Golgin 97 Protein (NBP2-38528PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (80%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Golgin 97 Antibody

  • gap junction protein, alpha 4, 37kD
  • golgi autoantigen, golgin subfamily a, 1
  • golgin A1
  • Golgin subfamily A member 1
  • Golgin-97
  • MGC33154

Background

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. The golgins are a family of proteins, of which the protein encoded by this gene is a member, that are localized to the Golgi. This encoded protein is associated with Sjogren's syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GOLGA1
Uniprot