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GOLGA3 Antibody

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Independent Antibodies: Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91953] - Staining of human colon, prostate, testis and tonsil using Anti-GOLGA3 antibody NBP1-91953 (A) shows similar protein ...read more
Western Blot: GOLGA3 Antibody [NBP1-91953] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/ Immunofluorescence: GOLGA3 Antibody [NBP1-91953] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus, nucleoli & the Golgi apparatus.
Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91953] - Staining of human tonsil shows moderate to strong cytoplasmic positivity in germinal center cells.
Western Blot: GOLGA3 Antibody [NBP1-91953] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91953] - Staining of human colon shows strong cytoplasmic positivity in lymphoid cells.
Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91953] - Staining of human prostate shows moderate granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91953] - Staining of human testis shows moderate to strong granular cytoplasmic positivity in cells in seminiferous ducts.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

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GOLGA3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TTLTSKLKASQAEISSLQSVRQWYQQQLALAQEARVRLQGEMAHIQVGQMTQAGLLEHLKLENVSLSQQLTETQHRSMKEKGRIAAQLQGIEADMLDQEAA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GOLGA3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
GOLGA3 Protein (NBP1-91953PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GOLGA3 Antibody

  • GCP170Golgi peripheral membrane protein
  • golgi autoantigen, golgin subfamily a, 3
  • Golgi complex-associated protein of 170 kD
  • Golgi complex-associated protein of 170 kDa
  • Golgi membrane associated protein
  • golgin A3
  • Golgin subfamily A member 3
  • golgin-160
  • golgin-165
  • male enhanced antigen-2
  • MEA-2
  • SY2/SY10 protein

Background

GOLGA3 is a member of the golgin family of proteins which are localized to the Golgi. It has been postulated to play a role in nuclear transport and Golgi apparatus localization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol GOLGA3