![Western Blot: GOLGA7 Antibody (2H8) [H00051125-M01] - GOLGA7 monoclonal antibody (M01), clone 2H8 Analysis of GOLGA7 expression in HL-60.](http://images.novusbio.com/fullsize/GOLGA7-Antibody-2H8-Western-Blot-H00051125-M01-img0005.jpg "Western Blot: GOLGA7 Antibody (2H8) [H00051125-M01] - GOLGA7 monoclonal antibody (M01), clone 2H8 Analysis of GOLGA7 expression in HL-60.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IP |
| Clone | 2H8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | GOLGA7 (AAH12032, 1 a.a. ~ 137 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MRPQQAPVSGKVFIQRDYSSGTRCQFQTKFPAELENRIDRQQFEETVRTLNNLYAEAEKLGGQSYLEGCLACLTAYTIFLCMETHYEKVLKKVSKYIQEQNEKIYAPQGLLLTDPIERGLRVIEITIYEDRGMSSGR |
| Specificity | GOLGA7 - golgi autoantigen, golgin subfamily a, 7 |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | GOLGA7 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
| Publications using H00051125-M01 | Applications | Species |
|---|---|---|
| Karolina Kuodyt? The Golgi complex as a regulatory platform for DNA Damage Response pathways Thesis 2023-01-01 (IP, MS, Human) | IP, MS | Human |
| Ren JG, Xing B, Lv K et al. RAB27B controls palmitoylation-dependent NRAS trafficking and signaling in myeloid leukemia The Journal of clinical investigation 2023-06-15 [PMID: 37317963] | ||
| Ko PJ, Woodrow C, Dubreuil MM et al. A ZDHHC5-GOLGA7 Protein Acyltransferase Complex Promotes Nonapoptotic Cell Death Cell Chem Biol 2019-10-17 [PMID: 31631010] | ||
| Cuiffo B, Ren R. Palmitoylation of oncogenic NRAS is essential for leukemogenesis. Blood 115(17):3598-605. 2010-04-29 [PMID: 20200357] |
Secondary Antibodies |
Isotype Controls |
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![Immunoprecipitation: GOLGA7 Antibody (2H8) [H00051125-M01] - Analysis of GOLGA7 transfected lysate using anti-GOLGA7 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GOLGA7 monoclonal antibody.](http://images.novusbio.com/fullsize/GOLGA7-Antibody-2H8-Immunoprecipitation-H00051125-M01-img0003.jpg "Immunoprecipitation: GOLGA7 Antibody (2H8) [H00051125-M01] - Analysis of GOLGA7 transfected lysate using anti-GOLGA7 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GOLGA7 monoclonal antibody.")
![ELISA: GOLGA7 Antibody (2H8) [H00051125-M01] - Detection limit for recombinant GST tagged GOLGA7 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GOLGA7-Antibody-2H8-ELISA-H00051125-M01-img0006.jpg "ELISA: GOLGA7 Antibody (2H8) [H00051125-M01] - Detection limit for recombinant GST tagged GOLGA7 is approximately 1ng/ml as a capture antibody.")
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