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Glutaredoxin 3/GLRX3 Antibody

Images

 
Western Blot: Glutaredoxin 3/GLRX3 Antibody [NBP2-20767] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Glutaredoxin 3/GLRX3 Antibody [NBP2-20767] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Glutaredoxin 3/GLRX3 Antibody [NBP2-20767] - Human breast carcinoma. TXNL2 antibody [N2C3] dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: Glutaredoxin 3/GLRX3 Antibody [NBP2-20767] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:10000.
Immunoprecipitation: Glutaredoxin 3/GLRX3 Antibody [NBP2-20767] - TXNL2 protein from 293T whole cell extracts using 5 ug of TXNL2 antibody [N2C3]. Western blot analysis was performed using TXNL2 antibody [N2C3]. ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Glutaredoxin 3/GLRX3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Glutaredoxin 3/GLRX3. The exact sequence is proprietary.
Localization
Cytoplasm; cell cortex
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GLRX3
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:1000-1:10000
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (80%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Glutaredoxin 3/GLRX3 Antibody

  • FLJ11864
  • GLRX3
  • GLRX4
  • Glutaredoxin 3
  • glutaredoxin 4
  • glutaredoxin 5
  • GRX3
  • GRX4
  • PICOT
  • PICOTbA500G10.4
  • PKC-interacting cousin of thioredoxin
  • PKCq-interacting protein
  • thioredoxin-like 2
  • TXNL2
  • TXNL3

Background

This gene encodes a member of the glutaredoxin family. Glutaredoxins are oxidoreductase enzymes that reduce a variety of substrates using glutathione as a cofactor. The encoded protein binds to and modulates the function of protein kinase C theta. The encoded protein may also inhibit apoptosis and play a role in cellular growth, and the expression of this gene may be a marker for cancer. Pseudogenes of this gene are located on the short arm of chromosomes 6 and 9. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol GLRX3
Uniprot