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FRA2 Antibody

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Western Blot: FRA2 Antibody [NBP1-89842] - Analysis in human cell lines U2OS and HEK293 using anti-FOSL2 antibody. Corresponding FOSL2 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.
Orthogonal Strategies: Western Blot: FRA2 Antibody [NBP1-89842] - Analysis in human cell lines U2OS and HEK293 using anti-FOSL2 antibody. Corresponding FOSL2 RNA-seq data are presented for the same cell lines. ...read more
Immunocytochemistry/ Immunofluorescence: FRA2 Antibody [NBP1-89842] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: FRA2 Antibody [NBP1-89842] - Staining of human stomach shows moderate to strong nuclear positivity in glandular cells.
Western Blot: FRA2 Antibody [NBP1-89842] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: FRA2 Antibody [NBP1-89842] - Staining of human cervix shows moderate to strong nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: FRA2 Antibody [NBP1-89842] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: FRA2 Antibody [NBP1-89842] - Staining of human skin shows moderate to strong nuclear positivity in squamous epithelial cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

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FRA2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EKLEFMLVAHGPVCKISPEERRSPPAPGLQPMRSGGGSVGAVVVKQEPLEEDSPSSSSAGLDKAQRSVIKPISIAGGFYGEEPLHTPIVVTSTPAVTPGTSNLVFTYPSVLEQESPASPSESCSKAHRRSSSSGDQSSDSL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FOSL2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
FRA2 Protein (NBP1-89842PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for FRA2 Antibody

  • FOS-like antigen 2
  • fos-related antigen 2
  • FRA-2
  • FRA2FLJ23306

Background

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol FOSL2