Home » Glutaredoxin 1/GLRX1 » Glutaredoxin 1/GLRX1 Antibodies » Glutaredoxin 1/GLRX1 Antibody [Unconjugated]
Novus Biologicals products are now on bio-techne.com

Glutaredoxin 1/GLRX1 Antibody [Unconjugated]

 (3 Publications)
 
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Glutaredoxin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3399) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Glutaredoxin 1 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and C2C12 mouse myoblast cell line. PVDF membrane ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Glutaredoxin 1/GLRX1 at approximately 10 kDa (as indicated) using 5 µg/mL ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3399-SP
100% Guarantee on every product

Glutaredoxin 1/GLRX1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GLRX1
Met1-Gln106
Accession # P35754
Specificity
Detects human and mouse GLRX1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GLRX
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using AF3399.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glutaredoxin 1/GLRX1 Antibody [Unconjugated]

  • GLRX
  • glutaredoxin (thioltransferase)
  • Glutaredoxin 1
  • glutaredoxin-1
  • GRXGRX1
  • MGC117407
  • Thioltransferase
  • thioltransferase-1
  • TTase-1

Background

Glutaredoxins or thioltransferases are a universally distributed family of small molecular weight proteins with thiol-disulfide exchange activity.Glutaredoxin 1(GLRX1/GRX) is a 12 kDa protein with glutathione-disulfide oxidoreductase activity. Glutaredoxins utilize the reducing power of glutathione to catalyze disulfide reductions in the presence of NADPH and glutathione reductase (the glutaredoxin system). The amino acid sequence of Glutaredoxin 1 in humans is highly homologous with that in other mammals (81-89%), and contains the conserved active sequence motif -Cys-Pro-Try-Cys-. Glutaredoxin 1 also catalyzes the formation of mixed disulfides (glutathionylation), which is an important redox regulatory mechanism, particularly in mammalian cells under oxidative stress conditions, to sense cellular redox potential.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Thioredoxin-1
Antibody - BSA Free
NBP2-52983
Flow Cytometry Thioredoxin-1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence Thioredoxin-1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence Thioredoxin-1 Antibody - BSA Free
(1 Publication)
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Glutaredoxin 2
Antibody
NBP1-90040
Immunocytochemistry/ Immunofluorescence Glutaredoxin 2 Antibody
Immunohistochemistry-Paraffin Glutaredoxin 2 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P
VAC14 Antibody
H00055697-B01P
Immunocytochemistry/ Immunofluorescence VAC14 Antibody
Western Blot VAC14 Antibody
Western Blot VAC14 Antibody
Species: Hu
Applications: ICC/IF, WB
Glutathione
Reductase Antibody
NBP2-16684
Western Blot Glutathione Reductase Antibody
Western Blot Glutathione Reductase Antibody
Western Blot Glutathione Reductase Antibody
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
Thioredoxin
Reductase 1/TRXR1 Ant ...
MAB7428
Western Blot Thioredoxin Reductase 1/TRXR1 Antibody (489804)
Intracellular Staining by Flow Cytometry Thioredoxin Reductase 1/TRXR1 Antibody (489804)
(5 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, ICFlow, WB
Peroxiredoxin 5
Antibody [Unconjugate
AF5724
Western Blot Peroxiredoxin 5 Antibody [Unconjugated]
Immunocytochemistry Peroxiredoxin 5 Antibody [Unconjugated]
Immunocytochemistry Peroxiredoxin 5 Antibody [Unconjugated]
Species: Hu, Mu, Rt
Applications: ICC, WB
Catalase Antibody
[Unconjugated]
AF3398
Western Blot Catalase Antibody [Unconjugated]
Simple Western Catalase Antibody [Unconjugated]
Knockout Validated Catalase Antibody [Unconjugated]
(3 Reviews)
(15 Publications)
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
Thioredoxin-2
Antibody
AF3254
Western Blot Thioredoxin-2 Antibody
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB
Glutaredoxin
3/GLRX3 Antibody [Unc ...
AF7560
Immunohistochemistry Glutaredoxin 3/GLRX3 Antibody [Unconjugated]
Western Blot Glutaredoxin 3/GLRX3 Antibody [Unconjugated]
Species: Hu, Mu, Rt
Applications: IHC, WB
ASK1 Antibody
NB100-56077
Immunohistochemistry-Paraffin ASK1 Antibody
Immunohistochemistry-Paraffin ASK1 Antibody
Species: Hu
Applications: IHC, IHC-P, IP, WB
TXNRD2 Antibody -
BSA Free
NBP2-94256
Immunohistochemistry-Paraffin TXNRD2 Antibody - BSA Free
Western Blot TXNRD2 Antibody - BSA Free
Immunohistochemistry-Paraffin TXNRD2 Antibody - BSA Free
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
Peroxiredoxin 6
Antibody (3A10-2A11)
H00009588-M01
ELISA Peroxiredoxin 6 Antibody (3A10-2A11)
Western Blot Peroxiredoxin 6 Antibody (3A10-2A11)
Western Blot Peroxiredoxin 6 Antibody (3A10-2A11)
(12 Publications)
Species: Bv, Hu
Applications: DB, ELISA, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(803 Publications)
Species: Hu
Applications: BA
KMT2B Antibody
NB300-864
Immunohistochemistry-Paraffin KMT2B Antibody
Immunohistochemistry-Paraffin KMT2B Antibody
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA
GAPDH Antibody
(1D4)
NB300-221
Western Blot GAPDH Antibody (1D4)
Immunocytochemistry/ Immunofluorescence GAPDH Antibody (1D4)
Immunocytochemistry/ Immunofluorescence GAPDH Antibody (1D4)
(19 Reviews)
(411 Publications)
Species: Av, Bv, Ca, Ch, ChHa, Dr, Eq, Fe, Ha, Hu, Mu, Ma-Op, Po, Pm, Rb, Rt, Sh, Ze
Applications: ChIP, ICC/IF, IHC, IHC-Fr, IP, KD, Simple Western, Single-Cell Western, WB
AKT [p Ser473]
Antibody [Unconjugate ...
AF887
Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
(49 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
GLYAT Antibody
NBP2-58917
Western Blot GLYAT Antibody
Immunohistochemistry-Paraffin GLYAT Antibody
Immunohistochemistry-Paraffin GLYAT Antibody
Species: Hu
Applications: IHC, IHC-P, WB
Glutaredoxin
1/GLRX1 Antibody [Unc ...
AF3399
Glutaredoxin 1/GLRX1 Antibody [Unconjugated]
Species: Hu, Mu
Applications: WB, Simple Western

Publications for Glutaredoxin 1/GLRX1 Antibody (AF3399)(3)


Reviews for Glutaredoxin 1/GLRX1 Antibody (AF3399) (0)

There are no reviews for Glutaredoxin 1/GLRX1 Antibody (AF3399). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Glutaredoxin 1/GLRX1 Antibody (AF3399) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Glutaredoxin 1/GLRX1 Products

Blogs on Glutaredoxin 1/GLRX1

There are no specific blogs for Glutaredoxin 1/GLRX1, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog #AF496,filled histogram) or Normal Goat IgG Isotype Control Antibody(Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugatedAnti-Goat IgG Secondary Antibody (Catalog #F0107).
Goat IgG Isotype Control [Unconjugated]
AB-108-C
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Glutaredoxin 1/GLRX1 Antibody [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol GLRX
Uniprot