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GIPC1 Antibody

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Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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GIPC1 Antibody Summary

Immunogen
Peptide with sequence C-GAIGDAKVGRY corresponding to C-Terminus according to NP_974197.1, NP_974199.1, NP_974196.1, NP_974198.1, NP_974223.1.
Localization
Cytoplasmic and Plasma membrane
Specificity
This antibody is expected to recognize both isoform 1 (NP_005707.1, NP_974197.1 and NP_974199.1) and isoform 2 (NP_974196.1, NP_974198.1 and NP_974223.1).
Predicted Species
Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GIPC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.035ug/ml
Application Notes
WB: Approx. 38 kDa band and strong 23-24 kDa band bands observed in lysates of HeLa, and approx. 24 kDa band also observed in human pancreas, kidney and muscle lysates (calculated MW of 36.0 kDa band according to NP_005707.1, NP_974197.1 and NP_974199.1 and 26.1 kDa band according to NP_974198.1 and NP_974223.1). In transfected HEK293 transiently expressing GIPC1 isoform 1 a band of approx. 40 kDa is observed. This band is not observed in the non-transfected HEK293.
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
GIPC1 Overexpression Lysate
HeLa Whole Cell Lysate
Publications
Read Publications using
NB100-1353 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GIPC1 Antibody

  • C19orf3chromosome 19 open reading frame 3
  • GAIP C-terminus-interacting protein
  • GIPC PDZ domain containing family, member 1
  • GIPCRGS-GAIP-interacting protein
  • GLUT1 C-terminal binding protein
  • GLUT1CBP
  • IGF-1 receptor interacting protein 1
  • MGC15889
  • MGC3774
  • NIP
  • PDZ domain-containing protein GIPC1
  • regulator of G-protein signalling 19 interacting protein 1
  • RGS19IP1IIP-1
  • SEMCAP
  • SYNECTIIN
  • SYNECTIN
  • Tax interaction protein 2
  • TIP-2RGS19-interacting protein 1

Background

GIPC1, GIPC2, and GIPC3 are a family of central PDZ-domain proteins with GH1 and GH2 domains. GIPC1 has been shown to be highly expressed in gastric, pancreatic, colorectal and lung cancer cell lines. It also has a list of identified binding partners in vitro including many transmembrane proteins and cell surface molecules involved in adhesion. In vivo, there is evidence that suggests a role of GIPC1 in transmembrane protein trafficking through the Golgi stacks and possibly a role in the later stages of vesicle trafficking.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GIPC1 Antibody (NB100-1353)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Human
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GIPC1 Antibody (NB100-1353) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Research Areas for GIPC1 Antibody (NB100-1353)

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Blogs on GIPC1

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Bioinformatics

Gene Symbol GIPC1
Entrez
Uniprot