Home » TGF-beta RIII » TGF-beta RIII Antibodies » TGF-beta RIII Antibody [Unconjugated]
Novus Biologicals products are now on bio-techne.com

TGF-beta RIII Antibody [Unconjugated]

 (18 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
TGF‑ beta RIII was detected in immersion fixed paraffin-embedded sections of human placenta tissue using Goat Anti-Human TGF‑ beta RIII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-242-PB) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface on syncytiotrophoblast cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
TGF‑ beta RIII was detected in immersion fixed paraffin-embedded sections of human placenta tissue using Goat Anti-Human TGF‑ beta RIII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-242-PB) at ...read more
PC‑3 human prostate cancer cell line was stained with Goat Anti-Human TGF‑ beta RIII Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF‑242‑PB, filled histogram) or isotype control antibody (Catalog ...read more
MCF-7 human breast adenocarcinoma cell line was stained with Goat Anti-Human TGF-beta RIII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-242-PB, filled histogram) or control antibody (AB-108-C, open ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF242
Phycoerythrin
FAB242P
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB242P
Carrier Free
AF-242-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

TGF-beta RIII Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human TGF‑ beta  RIII
Gly21-Asp781
Accession # AAA67061
Specificity
Detects human TGF-beta  RIII in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) TGF-beta  RIII is observed, and less than 1% cross-reactivity with recombinant human (rh) TGF-beta  RI, rhTGF-beta  RII, rhTGF-beta  RIIB, and rmTGF-beta  RI  is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TGFBR3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 1-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF-242-PB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF-beta RIII Antibody [Unconjugated]

  • betaglycan proteoglycan
  • Betaglycan
  • BGCAN
  • TBRIII
  • TGF-beta receptor type 3
  • TGF-beta receptor type III
  • TGF-beta RIII
  • TGFbetaRIII
  • TGFBR3
  • TGF-bRIII
  • TGFR-3
  • Transforming growth factor beta receptor III
  • transforming growth factor beta receptor type 3
  • transforming growth factor, beta receptor III (betaglycan, 300kDa)
  • transforming growth factor, beta receptor III

Background

Most cell types express three sizes of receptors for TGF-beta . These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type I receptor is a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta . The Type II receptor is also a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I and Type II receptors together form a heterodimeric signaling complex that is essential for the transduction of the anti-proliferative signals of TGF-beta .

The Type III receptor is a transmembrane proteoglycan with a large extracellular domain and a 43 amino acid residue cytoplasmic domain. The cytoplasmic domain of the Type III receptor lacks an obvious signaling motif and the receptor may not be involved directly in signal transduction. The Type III receptor binds TGF-beta 2 with the highest affinity. Other TGF-beta isoforms also bind the Type III receptor, but with lower affinities. Cellular responsiveness to TGF-beta 2 appears to be dependent on the presence of the Type III receptor which can interact with the signaling receptor complex. In addition to the transmembrane Type III receptor, a soluble form of the receptor is secreted by some cell types. The physiological role of this soluble receptor remains to be determined. The recombinant TGF-beta soluble receptor Type III binds the TGF-beta isoforms differentially in solution and exhibits TGF-beta antagonistic activities in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

TGF-beta RI/ALK-5
Antibody [Unconjugate ...
AF3025
Western Blot TGF-beta RI/ALK-5 Antibody [Unconjugated]
Immunocytochemistry TGF-beta RI/ALK-5 Antibody [Unconjugated]
Western Blot TGF-beta RI/ALK-5 Antibody [Unconjugated]
(16 Publications)
Species: Hu
Applications: ELISA, ICC, WB
TGF-beta 1
[Unconjugated]
7754-BH/CF
Bioactivity TGF-beta 1 [Unconjugated]
(53 Publications)
Species: Hu
Applications: BA
Endoglin/CD105
Antibody [Unconjugate
AF1320
Immunocytochemistry Endoglin/CD105 Antibody [Unconjugated]
Immunocytochemistry Endoglin/CD105 Antibody [Unconjugated]
Simple Western Endoglin/CD105 Antibody [Unconjugated]
(2 Reviews)
(44 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
TGF-beta 2
[Unconjugated]
302-B2
SDS-PAGE TGF-beta 2 [Unconjugated]
Bioactivity TGF-beta 2 [Unconjugated]
(86 Publications)
Species: Hu
Applications: BA
Smad2/3 Antibody
[Unconjugated]
AF3797
Western Blot Smad2/3 Antibody [Unconjugated]
Chromatin Immunoprecipitation (ChIP) Smad2/3 Antibody [Unconjugated]
Immunocytochemistry Smad2/3 Antibody [Unconjugated]
(1 Review)
(24 Publications)
Species: Hu, Mu
Applications: ChIP, ICC, IHC, Simple Western, WB
TGF-beta 1
[Unconjugated]
7754-BH/CF
Bioactivity TGF-beta 1 [Unconjugated]
(53 Publications)
Species: Hu
Applications: BA
CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
BRD2 Antibody
NBP1-30475
Immunohistochemistry-Paraffin BRD2 Antibody
Immunocytochemistry/ Immunofluorescence BRD2 Antibody
Immunoprecipitation BRD2 Antibody
(1 Review)
(3 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
FGF
basic/FGF2/bFGF [Unco
233-FB
FGF basic/FGF2/bFGF [Unconjugated]
(548 Publications)
Species: Hu
Applications: BA
NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free
(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
HLA DQ/DR/DP
Antibody (HLA-Pan/296 ...
NBP2-79843
Protein Array HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Western Blot HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
Decorin Antibody
[Unconjugated]
AF143
Western Blot Decorin Antibody [Unconjugated]
Simple Western Decorin Antibody [Unconjugated]
Immunohistochemistry Decorin Antibody [Unconjugated]
(29 Publications)
Species: Hu
Applications: IHC, Simple Western, WB
Syndecan-1/CD138
Antibody
AF2780
Immunohistochemistry Syndecan-1/CD138 Antibody
Immunohistochemistry Syndecan-1/CD138 Antibody
(1 Review)
(13 Publications)
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, WB
TGF-beta RII
Antibody [Unconjugate
AF-241-NA
Western Blot TGF-beta RII Antibody [Unconjugated]
Neutralization TGF-beta RII Antibody [Unconjugated]
Immunohistochemistry TGF-beta RII Antibody [Unconjugated]
(38 Publications)
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, IHC, Neut, WB
Fibronectin
Antibody - BSA Free
NBP1-91258
Western Blot Fibronectin Antibody - BSA Free
Western Blot Fibronectin Antibody - BSA Free
Western Blot Fibronectin Antibody - BSA Free
(11 Reviews)
(56 Publications)
Species: Bv, Ca, Eq, Fe, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
Activin
RIA/ALK-2/Activin Rec ...
AF637
Immunohistochemistry Activin RIA/ALK-2/Activin Receptor Type 1 Antibody
Flow Cytometry Activin RIA/ALK-2/Activin Receptor Type 1 Antibody
Immunohistochemistry Activin RIA/ALK-2/Activin Receptor Type 1 Antibody
(9 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, WB
TGF-beta 3
[Unconjugated]
243-B3
Bioactivity TGF-beta 3 [Unconjugated]
(123 Publications)
Species: Hu
Applications: BA
TGF-beta RIII
Antibody [Unconjugate
AF-242-PB
TGF-beta RIII Antibody [Unconjugated]
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready

Publications for TGF-beta RIII Antibody (AF-242-PB)(18)

We have publications tested in 3 confirmed species: Human, Rat, Rabbit.

We have publications tested in 7 applications: ELISA Development, Flow Cytometry, IHC, IHC-P, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
ELISA Development
(1)
Flow Cytometry
(3)
IHC
(2)
IHC-P
(1)
Immunoprecipitation
(1)
Neutralization
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(6)
Rat
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using AF-242-PB Applications Species
Keren Tazat, Leslie Pomeraniec-Abudy, Melissa Hector-Greene, Szabina Szófia Szilágyi, Swati Sharma, Elise M. Cai et al. ALK1 regulates the internalization of endoglin and the type III TGF-beta receptor Molecular Biology of the Cell 2021-04-01 [PMID: 33566682]
F Facciotti, P Larghi, R Bosotti, C Vasco, N Gagliani, C Cordiglier, S Mazzara, V Ranzani, E Rottoli, S Curti, A Penatti, B Karnani, Y Kobayashi, M Crosti, M Bombaci, JP van Hambur, G Rossetti, R Gualtierot, M Gerosa, S Gatti, S Torretta, L Pignataro, SW Tas, S Abrignani, M Pagani, F Grassi, PL Meroni, RA Flavell, J Geginat Evidence for a pathogenic role of extrafollicular, IL-10-producing CCR6+B helper T cells in systemic lupus erythematosus Proc. Natl. Acad. Sci. U.S.A., 2020-03-17;117(13):7305-7316. 2020-03-17 [PMID: 32184325] (Flow Cytometry, Human) Flow Cytometry Human
Karthikeyan Mythreye Altering the Proteoglycan State of Transforming Growth Factor ? Type III Receptor (T?RIII)/Betaglycan Modulates Canonical Wnt/?-Catenin Signaling J. Biol. Chem., 2016-10-26;291(49):25716-25728. 2016-10-26 [PMID: 27784788] (Immunoprecipitation, Western Blot) Immunoprecipitation, Western Blot
Shanmugarajan Krishnan, Emese Szabo, Isabel Burghardt, Karl Frei, Ghazaleh Tabatabai, Michael Weller Modulation of cerebral endothelial cell function by TGF-beta in glioblastoma: VEGF-dependent angiogenesis versus endothelial mesenchymal transition Oncotarget 2015-06-15 [PMID: 26090865]
Drasin D, Guarnieri A, Neelakantan D, Kim J, Cabrera J, Wang C, Zaberezhnyy V, Gasparini P, Cascione L, Huebner K, Tan A, Ford H TWIST1-Induced miR-424 Reversibly Drives Mesenchymal Programming while Inhibiting Tumor Initiation. Cancer Res, 2015-02-25;75(9):1908-21. 2015-02-25 [PMID: 25716682] (Flow Cytometry, Human) Flow Cytometry Human
Catherine E. Gatza, Jennifer L. Elderbroom, Sun Young Oh, Mark D. Starr, Andrew B. Nixon, Gerard C. Blobe The Balance of Cell Surface and Soluble Type III TGF-beta Receptor Regulates BMP Signaling in Normal and Cancerous Mammary Epithelial Cells1 Neoplasia 2014-07-26 [PMID: 25077702]
Knelson EH, Gaviglio AL, Nee JC et al. Stromal heparan sulfate differentiates neuroblasts to suppress neuroblastoma growth. J Clin Invest 7/1/2014 [PMID: 24937430]
Alison E. Meyer, Catherine E. Gatza, Tam How, Mark Starr, Andrew B. Nixon, Gerard C. Blobe Role of TGF-beta receptor III localization in polarity and breast cancer progression Molecular Biology of the Cell 2014-08-01 [PMID: 24870032]
Wang F, Zhou Y, Fu B, Wu Y, Zhang R, Sun R, Tian Z, Wei H Molecular signatures and transcriptional regulatory networks of human immature decidual NK and mature peripheral NK cells. Eur J Immunol, 2014-06-11;44(9):2771-84. 2014-06-11 [PMID: 24838931] (Flow Cytometry, Human) Flow Cytometry Human
Erik H. Knelson, Angela L. Gaviglio, Alok K. Tewari, Michael B. Armstrong, Karthikeyan Mythreye, Gerard C. Blobe Type III TGF-β receptor promotes FGF2-mediated neuronal differentiation in neuroblastoma Journal of Clinical Investigation 2013-11-01 [PMID: 24216509]
Show All 18 Publications.

Reviews for TGF-beta RIII Antibody (AF-242-PB) (0)

There are no reviews for TGF-beta RIII Antibody (AF-242-PB). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TGF-beta RIII Antibody (AF-242-PB) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional TGF-beta RIII Products

Blogs on TGF-beta RIII.

Check out the latest blog posts on TGF-beta RIII.
TGF-beta RIII - a high affinity reservoir for TGF-beta I/II ligands with therapeutic potential
TGF beta (transforming growth factor beta) is a superfamily of cytokines that participate in a variety of cellular processes including growth, proliferation, differentiation, and apoptosis. There are 3 classes of receptors for TGF beta cytokines an...  Read full blog post.
TGF-beta RIII - A multi-functional regulator of the TGF-beta signaling pathway
Transforming growth factor-beta receptor III (TGF-beta RIII) is one of three receptors for the secreted growth factor TGF-beta. Unlike type I and type II TGF-beta receptors, TGF-beta RIII does not participate directly in the propagation of intracel...  Read full blog post.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TGF-beta RIII Antibody [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol TGFBR3
Uniprot