GFAP Antibody

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Glial fibrillary acidic protein (GFAP) is a class III intermediate filament protein that is largely expressed in astrocytes in addition to non-myelinating Schwann cells and glial cells (1,2). Other members of the type III intermediate filament family include desmin, peripherin, and vimentin (2-4). GFAP was first identified in the brains of multiple sclerosis patients (2). Human GFAP protein is 432 amino acids in length with a theoretical molecular weight of ~50 kDa (1,3). GFAP has at least 10 known isoforms, with the most prevalent and common in the brain being GFAP-alpha which is made of a head domain, a rod domain with four coils (1A, 1B, 2A, 2B) joined by linker regions, and a tail domain (1). GFAP is a marker of mature astrocytes, but is also expressed throughout development in both fetal and adult neural stem cells (2). While the exact function of GFAP is still elusive, it has been shown to play a role in cellular processes such as migration, mitosis, structural integrity, and signaling (2).

An increase in GFAP levels is often associated with neuroinflammation which results in the activation and proliferation of astroglia cell population (1,2). GFAP expression is also observed in brains of patients with neurodegenerative diseases including Alzheimer's and Parkinson's, epilepsy disorders, and brain injuries (1-4). Lesion sites associated with neurodegeneration can exhibit an array of gliosis characteristics from glial scarring with reduced astrocyte proliferation to activated, GFAP-positive astrocytes surrounding amyloid plaques (2). Furthermore, the GFAP gene is a target of single nucleotide polymorphisms in the coding region, considered a gain-of-function mutation, characterized by astrocytic inclusions, termed Rosenthal fibers, resulting in Alexander Disease (1-4). GFAP is also a center of many post-translational modifications, such as phosphorylation, which can alter various aspects of filament assembly (1,4).

References

1. Yang, Z., & Wang, K. K. (2015). Glial fibrillary acidic protein: from intermediate filament assembly and gliosis to neurobiomarker. Trends in Neurosciences. https://doi.org/10.1016/j.tins.2015.04.003

2. Hol, E. M., & Capetanaki, Y. (2017). Type III Intermediate Filaments Desmin, Glial Fibrillary Acidic Protein (GFAP), Vimentin, and Peripherin. Cold Spring Harbor Perspectives in Biology. https://doi.org/10.1101/cshperspect.a021642

3. Potokar, M., Morita, M., Wiche, G., & Jorgacevski, J. (2020). The Diversity of Intermediate Filaments in Astrocytes. Cells. https://doi.org/10.3390/cells9071604

4. Viedma-Poyatos, a., Pajares, M. A., & Perez-Sala, D. (2020). Type III intermediate filaments as targets and effectors of electrophiles and oxidants. Redox Biology. https://doi.org/10.1016/j.redox.2020.101582

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: ICC/IF, IF/IHC, IHC, IHC-F, IHC-Fr, MI, WB.

Showing Publications 1 - 10 of 43. Show All 43 Publications. Collapse Publications.

Publications using NBP1-05198	Applications	Species
Wu DM, Liu JP, Liu J et al. Immune pathway activation in neurons triggers neural damage after stroke Cell reports 2023-11-01 [PMID: 37917581] (IHC, Mouse)	IHC	Mouse
Presto P, Ji G, Ponomareva O et al. Hmgb1 Silencing in the Amygdala Inhibits Pain-Related Behaviors in a Rat Model of Neuropathic Pain International Journal of Molecular Sciences 2023-07-26 [PMID: 37569320] (IHC-Fr, Rat) Details: Dilutions: 1:1000	IHC-Fr	Rat
Li L, Sun Y, Davis AE et al. Mettl14-mediated m6A modification ensures the cell-cycle progression of late-born retinal progenitor cells Cell reports 2023-06-01 [PMID: 37269288]
Shipeng Li, Wenfeng Han, Qichen He, Yang Wang, Gang Jin, Youcheng Zhang Ginsenoside Rh2 suppresses colon cancer growth by targeting the miR-150-3p/SRCIN1/Wnt axis. Acta biochimica et biophysica Sinica 2023-05-17 [PMID: 36916297]
Tang AT, Buchholz DW, Szigety KM et al. Cell-autonomous requirement for ACE2 across organs in lethal mouse SARS-CoV-2 infection PLoS biology 2023-02-01 [PMID: 36745682] (IHC, Mouse)	IHC	Mouse
Badr M, McFleder RL, Wu J et al. Expansion of regulatory T cells by CD28 superagonistic antibodies attenuates neurodegeneration in A53T- alpha -synuclein Parkinson's disease mice Journal of neuroinflammation 2022-12-31 [PMID: 36587195] (IHC-Fr, Mouse) Details: Dilution used in IHC-Fr 1:5,000	IHC-Fr	Mouse
Freel BA, Kelvington BA, Sengupta S et al. Sterol dysregulation in Smith-Lemli-Opitz syndrome causes astrocyte immune reactivity through microglia crosstalk Disease models & mechanisms 2022-12-01 [PMID: 36524414] (ICC/IF, Mouse)	ICC/IF	Mouse
Asare-Bediako B, Adu-Agyeiwaah Y, Abad A et al. Hematopoietic Cells Influence Vascular Development in the Retina Cells 2022-10-13 [PMID: 36291075] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Bin Imtiaz MK, Royall LN, Gonzalez-Bohorquez D, Jessberger S. Human neural progenitors establish a diffusion barrier in the endoplasmic reticulum membrane during cell division Development 2022-10-15 [PMID: 35815653]
Rauschenberger L, Behnke J, Grotemeyer A et al. Age-dependent neurodegeneration and neuroinflammation in a genetic A30P/A53T double-mutated alpha-synuclein mouse model of Parkinson's disease Neurobiology of disease 2022-06-21 [PMID: 35750147] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Peng L, Liu S, Xu J et al. Metformin Alleviates Prolonged Isoflurane Inhalation Induced Cognitive Decline Via Reducing Neuroinflammation in Adult Mice Int Immunopharmacol 2022-06-16 [PMID: 35709590]
De A, Morales JE, Chen Z et al. The b8 Integrin cytoplasmic domain activates extracellular matrix adhesion to promote brain neurovascular development Development (Cambridge, England) 2022-02-25 [PMID: 35217866]
Boutin ME, Strong CE, Van Hese B et al. A multiparametric calcium signal screening platform using iPSC-derived cortical neural spheroids SLAS discovery : advancing life sciences R & D 2022-01-26 [PMID: 35092840] (ICC/IF, Human)	ICC/IF	Human
Cole JD, Sarabia Del Castillo J, Gut G et al. Characterization of the neurogenic niche in the aging dentate gyrus using iterative immunofluorescence imaging eLife 2022-01-28 [PMID: 35089129] (IF/IHC, Human, Mouse)	IF/IHC	Human, Mouse
Sandhurst ES, Jaswandkar SV, Kundu K et al. Nanoarchitectonics of a Microsphere-Based Scaffold for Modeling Neurodevelopment and Neurological Disease ACS applied bio materials 2022-01-19 [PMID: 35045249] (ICC/IF, Human)	ICC/IF	Human
Morales JE, De A, Miller AA et al. Mlc1-Expressing Perivascular Astrocytes Promote Blood-Brain Barrier Integrity The Journal of neuroscience : the official journal of the Society for Neuroscience 2021-12-17 [PMID: 34965971]
Khan SS, Sobu Y, Dhekne HS Et al. Pathogenic LRRK2 control of primary cilia and Hedgehog signaling in neurons and astrocytes of mouse brain eLife 2021-10-18 [PMID: 34658337] (Mouse)		Mouse
Denoth-Lippuner A, Jaeger BN, Liang T et al. Visualization of individual cell division history in complex tissues using iCOUNT Cell stem cell 2021-09-08 [PMID: 34525348] (MI, Mouse)	MI	Mouse
Logan T, Simon MJ, Rana A Et al. Rescue of a lysosomal storage disorder caused by Grn loss of function with a brain penetrant progranulin biologic Cell 2021-08-25 [PMID: 34450028] (IF/IHC, Mouse)	IF/IHC	Mouse
Chen H, Kevala K, Aflaki E Et Al. GPR110 ligands reduce chronic optic tract gliosis and visual deficit following repetitive mild traumatic brain injury in mice Journal of neuroinflammation 2021-07-17 [PMID: 34273979] (IHC-Fr)	IHC-Fr
Mausner-Fainberg K, Benhamou M, Golan M et al. Specific Blockade of Bone Morphogenetic Protein-2/4 Induces Oligodendrogenesis and Remyelination in Demyelinating Disorders Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 2021-06-22 [PMID: 34159538]
Sethi P, Virmani G, Gupta K et al. Automated morphometric analysis by SMorph reveals plasticity induced by antidepressant therapy in hippocampal astrocytes Journal of cell science 2021-04-30 [PMID: 33929531]
Li J, Tian M, Hua T et al. Combination of autophagy and NFE2L2/NRF2 activation as a treatment approach for neuropathic pain Autophagy 2021-04-09 [PMID: 33834930]
Niehaus JK, Taylor-Blake B, Loo L et al. Spinal macrophages resolve nociceptive hypersensitivity after peripheral injury Neuron 2021-02-24 [PMID: 33667343]
Tao K, Cai Q, Zhang X et al. Astrocytic histone deacetylase 2 facilitates delayed depression and memory impairment after subarachnoid hemorrhage by negatively regulating glutamate transporter-1 Ann Transl Med 2020-06-01 [PMID: 32617311] (ICC/IF, Mouse)	ICC/IF	Mouse
Liu, Z, Murphy, S F Et al. Neuronal/astrocytic expression of chemokine (C-C motif) ligand 2 is associated with monocyte/macrophage recruitment in male chronic pelvic pain. Pain 2020-11-01 [PMID: 32541388] (WB, Human)	WB	Human
Wu KY, Tang FL, Lee D et al. Ependymal Vps35 promotes ependymal cell differentiation and survival, suppresses microglial activation, and prevents neonatal hydrocephalus J. Neurosci. 2020-04-08 [PMID: 32291328]
Cougnoux A, Drummond RA, Fellmeth M, et al. Unique molecular signature in mucolipidosis type IV microglia J Neuroinflammation 2019-12-28 [PMID: 31883529] (ICC/IF, Mouse)	ICC/IF	Mouse
Cougnoux A, Fellmeth M, Gu T et al. Maternal immune activation modifies the course of Niemann-pick disease, type C1 in a gender specific manner Mol. Genet. Metab. 2019-10-17 [PMID: 31668555] (IF/IHC, Mouse)	IF/IHC	Mouse
Zhang Y, Zhang Y, Wu R et al. Effect of cerebral small vessel disease on cognitive function and TLR4 expression in hippocampus J Clin Neurosci 2019-07-06 [PMID: 31288981]
Jung S, Choe S, Woo H et al. Autophagic death of neural stem cells mediates chronic stress-induced decline of adult hippocampal neurogenesis and cognitive deficits Autophagy 2019-06-24 [PMID: 31234698] (IHC-F, Mouse)	IHC-F	Mouse
Majumder S, Ren L, Pushpakumar S, Sen U Autophagy Mediates Astrogenesis in Adult Hippocampal Neural Stem Cells Exp Neurobiol 2019-04-01 [PMID: 31138991] (WB, ICC/IF, Human)	WB, ICC/IF	Human
McWilliams IL, Kielczewski JL, Ireland DDC et al. Pseudovirus rVSVDG-ZEBOV-GP Infects Neurons in Retina and CNS, Causing Apoptosis and Neurodegeneration in Neonatal Mice Cell Rep 2019-02-12 [PMID: 30759384] (ICC/IF, Mouse)	ICC/IF	Mouse
JW Lee, H Nam, LE Kim, Y Jeon, H Min, S Ha, Y Lee, SY Kim, SJ Lee, EK Kim, SW Yu TLR4 (toll-like receptor 4) activation suppresses autophagy through inhibition of FOXO3 and impairs phagocytic capacity of microglia Autophagy, 2018-12-13;0(0):. 2018-12-13 [PMID: 30523761]
Dhekne HS, Yanatori I, Gomez RC et al. A pathway for Parkinson's Disease LRRK2 kinase to block primary cilia and Sonic hedgehog signaling in the brain. eLife. [PMID: 30398148] (ICC/IF, Human)	ICC/IF	Human
Arzmi MH, Cirillo N, Lenzo JC, Catmull DV. Necroptosis inhibition as a therapy for Niemann-Pick disease, type C1: Inhibition of RIP kinases and combination therapy with 2-hydroxypropyl-Beta-cyclodextrin. Molecular Genetics and Metabolism. 2018-12-01 [PMID: 30392741] (IF/IHC, Mouse)	IF/IHC	Mouse
Gaire J, Lee H, Hilborn N et al. The role of inflammation on the functionality of intracortical microelectrodes. J. Neural Eng. 2018-09-27 [PMID: 30260321] (IHC-Fr, Rat)	IHC-Fr	Rat
Anderson MA, O'Shea TM, Burda JE et al. Required growth facilitators propel axon regeneration across complete spinal cord injury Nature 2018-08-31 [PMID: 30158698] (ICC/IF, Rat, Mouse)	ICC/IF	Rat, Mouse
Kumar NN, Lochhead JJ, Pizzo ME et al. Delivery of immunoglobulin G antibodies to the rat nervous system following intranasal administration: Distribution, dose-response, and mechanisms of delivery J Control Release 2018-08-04 [PMID: 30081144] (ICC/IF, Rat)	ICC/IF	Rat
Chavez-Valdez R, Emerson P, Goffigan-Holmes J et al. Delayed injury of hippocampal interneurons after neonatal hypoxia-ischemia and therapeutic hypothermia in a murine model Hippocampus 2018-05-21 [PMID: 29781223] (Mouse)		Mouse
Bhatti F, Ball G, Hobbs R et al. Pulmonary Surfactant Protein A Is Expressed in the Mouse Retina by Muller Cells and Impacts neovascularization in Oxygen Induced Retinopathy. Invest. Ophthalmol. Vis. Sci. 2014-11-18 [PMID: 25406276]
Bright A Differential Recruitment of Bone Marrow-Derived Cells into the Injured Retina Thesis 2023-01-01
Fort T Inefficacy of n-acetylcysteine in mitigating cue-induced relapse to amphetamine Thesis 2022-01-01
Show All 43 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	4 reviewed by: Verified Customer	IHC-Fr	Canine	03/09/2018	View Summary Application Immunohistochemistry-Frozen Sample Tested Canine Retina Species Canine Lot 7529-19

Array NBP1-05198

• GFAP Antibodies
• GFAP ELISA Kits
• GFAP Lysate
• GFAP Proteins
• GFAP ELISA