I am interested in native or recombinant GFAP protein without GST Tag or in urea buffer. Can I use your GFAP antibody (NB100-53809) to detect P0809, P0810 and P1691 GFAP proteins?

Name: GFAP Antibody (273807) [Unconjugated]
Brand: Novus Biologicals
SKU: MAB2594
Rating: 4.5 (2 reviews)

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Accepted Answer

P0809, P0810 and P1691 are blocking peptides that are not suitable for use with NB100-53809. Unfortunately, we do not carry a GFAP protein without a GST tag. I apologize for the inconvenience.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, Simple Western, ICC/IF, KO
Clone	273807
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Formulation	Size	Price
Carrier Free MAB2594-SP

Immunogen	E. coli-derived recombinant human GFAP Leu292-Met432 Accession # P14136
Specificity	Detects human GFAP in direct ELISAs and Western blots.
Source	N/A
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	GFAP
Purity Statement	Protein A or G purified from hybridoma culture supernatant
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.5 mg/mL in sterile PBS.

GFAP Antibody (273807) [Unconjugated]

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Publications for GFAP Antibody (MAB2594)(8)

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Publications using MAB2594	Applications	Species
Philip Dettinger, Tobias Kull, Geethika Arekatla, Nouraiz Ahmed, Yang Zhang, Florin Schneiter et al. Open-source personal pipetting robots with live-cell incubation and microscopy compatibility Nature Communications 2022-05-30 [PMID: 35637179]
Cruz-Orengo L, Daniels B, Dorsey D, Basak S, Grajales-Reyes J, McCandless E, Piccio L, Schmidt R, Cross A, Crosby S, Klein R Enhanced sphingosine-1-phosphate receptor 2 expression underlies female CNS autoimmunity susceptibility. J Clin Invest, 2014-05-08;124(6):2571-84. 2014-05-08 [PMID: 24812668] (IHC, Human)	IHC	Human
Zeng , L, He , X, Wang , Y, Tang , Y, Zheng , C, Cai , H, Liu , J, Wang , Y, Fu , Y, Yang , G-Y MicroRNA-210 overexpression induces angiogenesis and neurogenesis in the normal adult mouse brain. Gene Ther, 2013-10-24;21(1):37-43. 2013-10-24 [PMID: 24152581] (IHC, Mouse)	IHC	Mouse
Hamalainen RH, Manninen T, Koivumaki H et al. Tissue- and cell-type-specific manifestations of heteroplasmic mtDNA 3243A>G mutation in human induced pluripotent stem cell-derived disease model. Proc Natl Acad Sci U S A. 9/17/2013 [PMID: 24003133]
Mangiola , Annunzia, Saulnier , Nathalie, De Bonis , Pasquale, Orteschi , Daniela, Sica , Gigliola, Lama , Gina, Pettorini , Benedett, Sabatino , Giovanni, Zollino , Marcella, Lauriola , Libero, Colabianchi , Anna, Proietti , Gabriell, Kovacs , Gyula, Maira , Giulio, Anile , Carmelo Gene expression profile of glioblastoma peritumoral tissue: an ex vivo study. PLoS ONE, 2013-03-05;8(3):e57145. 2013-03-05 [PMID: 23472076] (IHC, Human)	IHC	Human
Walter D, Satheesha S, Albrecht P, Bornhauser BC, D'Alessandro V, Oesch SM, Rehrauer H, Leuschner I, Koscielniak E, Gengler C, Moch H, Bernasconi M, Niggli FK, Schafer BW CD133 positive embryonal rhabdomyosarcoma stem-like cell population is enriched in rhabdospheres. PLoS ONE, 2011-05-13;6(5):e19506. 2011-05-13 [PMID: 21602936] (ICC, Human)	ICC	Human
Thon N, Damianoff K, Hegermann J, Grau S, Krebs B, Schnell O, Tonn JC, Goldbrunner R Presence of pluripotent CD133+ cells correlates with malignancy of gliomas. Mol. Cell. Neurosci., 2008-08-07;43(1):51-9. 2008-08-07 [PMID: 18761091] (ICC, Human)	ICC	Human
Ponomarev ED, Novikova M, Maresz K, Shriver LP, Dittel BN Development of a culture system that supports adult microglial cell proliferation and maintenance in the resting state. J. Immunol. Methods, 2005-04-26;300(1):32-46. 2005-04-26 [PMID: 15893321] (ICC, Mouse)	ICC	Mouse

	Verified Customer 06/07/2017
Application:	ICC
Species:	Pig

	Verified Customer 02/19/2015
Application:	IF
Species:	Human

Gene Symbol	GFAP
Uniprot	P14136()