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Recombinant Human GDF-6/BMP-13 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Concentration
LYOPH

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Recombinant Human GDF-6/BMP-13 Protein Summary

Description
A disulfide-linked homodimeric protein containing two 120 amino acid chains corresponding to GDF-6/BMP-13 Source: E. coli

Uniprot ID: Q6KF10

Amino Acid Sequence: TAFASRHGKR HGKKSRLRCS KKPLHVNFKE LGWDDWIIAP LEYEAYHCEG VCDFPLRSHL EPTNHAIIQT LMNSMDPGST PPSCCVPTKL TPISILYIDA GNNVVYKQYE DMVVESCGCR
Details of Functionality
Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 2.0 ug/ml, corresponding to a specific activity of > 500 IU/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
GDF6
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 0.1 EU/ug of GDF-6/BMP-13 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
27.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Human GDF-6/BMP-13 Protein

  • BMP13
  • BMP-13
  • CDMP2
  • GDF16
  • GDF6
  • GDF-6
  • growth differentiation factor 6
  • Growth/differentiation factor 16
  • growth/differentiation factor 6
  • KFS
  • KFS1
  • KFSL
  • MCOP4
  • MCOPCB6
  • MGC158100
  • MGC158101
  • SCDO4
  • SGM1

Background

GDF6 encodes a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily of secreted signaling molecules. It is required for normal formation of some bones and joints in the limbs, skull, and axial skeleton. Mutations in this gene result in colobomata, which are congenital abnormalities in ocular development, and in Klippel-Feil syndrome (KFS), which is a congenital disorder of spinal segmentation. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol GDF6