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Human GATA-4 ELISA Kit (Colorimetric)

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ELISA: Human GATA-4 ELISA Kit (Colorimetric) [NBP3-00389] - Samples were spiked with high concentrations of Human GATA-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the ...read more
ELISA: Human GATA-4 ELISA Kit (Colorimetric) [NBP3-00389] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
1.56 - 100 ng/mL
Sensitivity
0.94 ng/mL

Order Details

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Human GATA-4 ELISA Kit (Colorimetric) Summary

Standard Curve Range
1.56 - 100 ng/mL
Sensitivity
0.94 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
%CV < 5.77
Intra-Assay
%CV < 6.76
Spike Recovery
91 - 107%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
GATA4

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human GATA-4 ELISA Kit (Colorimetric)

  • GATA binding protein 4
  • GATA4
  • GATA-4
  • GATA-binding factor 4
  • GATA-binding protein 4
  • MGC126629
  • transcription factor GATA-4

Background

GATA4 is a 46 kDa member of the GATA family of zinc finger containing transcription factors that is involved in the development of cardiac hypertrophy and remodeling, and plays a critical role in regulating basal and agonist or stress induced gene expression in cardiac and smooth muscle cell types. GATA4 contains a conserved MAPK phosphorylation site at serine 105 within the transcriptional activation domain. Serine 105 of GATA4 is phosphorylated in response to agonist stimulation through MEK 1 ERK1 / 2, and weakly through JNK or p38 MAPKs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol GATA4