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Recombinant Human Gankyrin Protein

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SDS-Page: Recombinant Human Gankyrin Protein [NBC1-18518] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
SDS-Page: Recombinant Human Gankyrin Protein [NBC1-18518] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

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Recombinant Human Gankyrin Protein Summary

Description
An un-tagged recombinant protein corresponding to the amino acids 1-226 of Human Gankyrin

Source: E.coli

Amino Acid Sequence: MEGCVSNLMV CNLAYSGKLE ELKESILADK SLATRTDQDS RTALHWACSA GHTEIVEFLL QLGVPVNDKD DAGWSPLHIA ASAGRDEIVK ALLGKGAQVN AVNQNGCTPL HYAASKNRHE IAVMLLEGGA NPDAKDHYEA TAMHRAAAKG NLKMIHILLY YKASTNIQDT EGNTPLHLAC DEERVEEAKL LVSQGASIYI ENKEEKTPLQ VAKGGLGLIL KRMVEG
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
PSMD10
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
24.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human Gankyrin Protein

  • 26S proteasome non-ATPase regulatory subunit 10
  • 26S proteasome regulatory subunit p28
  • ankyrin repeat protein
  • dJ889N15.2
  • gankyrin
  • hepatocellular carcinoma-associated protein p28-II
  • p28
  • p28(GANK)
  • proteasome (prosome, macropain) 26S subunit, non-ATPase, 10

Background

Gankyrin is an oncoprotein commonly overexpressed in most hepatocellular carcinomas. Gankyrin interacts with S6 ATPase of the 19S regulatory particle of the 26S proteasome. It is known to play a role in the regulation of the phosphorylation of the retinoblastoma protein by CDK4, and to enhance the ubiquitinylation of p53 by MDM2. Gankyrin consists of 7 ankyrin repeats and is structurally similar to I kappa Bs. Recombinant human Gankyrin was expressed in E.coli and purified by conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol PSMD10