![Western Blot: gamma Catenin Antibody [NB100-1060] - Staining (1ug/ml) of Rat Eye lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/gamma-Catenin-Antibody-Western-Blot-NB100-1060-img0004.jpg "Western Blot: gamma Catenin Antibody [NB100-1060] - Staining (1ug/ml) of Rat Eye lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
| Reactivity | Hu, Mu, Rt, Po, CaSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-GLRPPYPTADHMLA corresponding to C-Terminus according to NP_002221.1, NP_068831.1. |
| Localization | Cytoplasmic in a soluble and membrane-associated form. |
| Specificity | This antibody is expected to recognise both reported isoforms, as represented by NP_002221.1 and NP_068831.1. |
| Predicted Species | Porcine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | JUP |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | EIA: An anonymous customer has reported successful usage quantitative ELISA assays (EIA), very sensitive to freeze/thaw cycles. WB: Approx. 85 kDa band observed in lysates of A431A 549 and NIH3T3 and in rat eye lysates (calculated MW of 81.7 kDa band according to human NP_002221.1 and mouse NP_034723.1). IHC-Paraffin embedded Human Heart. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
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| Publication using NB100-1060 | Applications | Species |
|---|---|---|
| Knudsen KA, Wheelock MJ. Plakoglobin, or an 83-kD homologue distinct from beta-catenin, interacts with E-cadherin and N-cadherin. J Cell Biol 1992-08-01 [PMID: 1639850] |
Secondary Antibodies |
Isotype Controls |
Research Areas for gamma Catenin Antibody (NB100-1060)Find related products by research area.
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Beta Catenin in Cell Adhesion and T-cell Signaling Beta Catenin is a cytosolic, 88 kDa intracellular protein that tightly associates with cell surface cadherin glycoproteins. It is one member of the catenin family that includes alpha Catenin, beta Catenin, and gamma Catenin. Colocalization studies usi... Read full blog post. |
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![Immunohistochemistry-Paraffin: gamma Catenin Antibody [NB100-1060] - Staining of Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/gamma-Catenin-Antibody-Immunohistochemistry-Paraffin-NB100-1060-img0005.jpg "Immunohistochemistry-Paraffin: gamma Catenin Antibody [NB100-1060] - Staining of Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Western Blot: gamma Catenin Antibody [NB100-1060] - Staining of A431 (A) and NIH3T3 (B) cell lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/gamma-Catenin-Antibody-Western-Blot-NB100-1060-img0003.jpg "Western Blot: gamma Catenin Antibody [NB100-1060] - Staining of A431 (A) and NIH3T3 (B) cell lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
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