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CRTH-2/GPR44 Antibody - BSA Free

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Western Blot: CRTH-2/GPR44 Antibody [NBP1-76755] - Jurkat cell lysate with GPR44 antibody at 1 ug/ml in (A) the absence and (B) presence of blocking peptide.
Western Blot: CRTH-2/GPR44 Antibody [NBP1-76755] - Jurkat cell lysate with GPR44 antibody at 1 ug/ml in (A) the absence and (B) presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: CRTH-2/GPR44 Antibody [NBP1-76755] - Human Heart tissue with CRTH2 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: CRTH-2/GPR44 Antibody [NBP1-76755] - Human heart tissue with GPR44 antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, GpSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-76755AF350
Alexa Fluor 405
NBP1-76755AF405
Alexa Fluor 488
NBP1-76755AF488
Alexa Fluor 532
NBP1-76755AF532
Alexa Fluor 594
NBP1-76755AF594
Alexa Fluor 647
NBP1-76755AF647
Alexa Fluor 700
NBP1-76755AF700
Alexa Fluor 750
NBP1-76755AF750
Biotin
NBP1-76755B
CoraFluor 1
NBP1-76755CL1
DyLight 350
NBP1-76755UV
DyLight 405
NBP1-76755V
DyLight 488
NBP1-76755G
DyLight 550
NBP1-76755R
DyLight 594
NBP1-76755DL594
DyLight 650
NBP1-76755C
DyLight 680
NBP1-76755FR
DyLight 755
NBP1-76755IR
FITC
NBP1-76755F
HRP
NBP1-76755H
Janelia Fluor 525
NBP1-76755JF525
Janelia Fluor 549
NBP1-76755JF549
Janelia Fluor 585
NBP1-76755JF585
Janelia Fluor 635
NBP1-76755JF635
Janelia Fluor 646
NBP1-76755JF646
Janelia Fluor 669
NBP1-76755JF669
PerCP
NBP1-76755PCP
mFluor Violet 450 SE
NBP1-76755MFV450
mFluor Violet 500 SE
NBP1-76755MFV500
mFluor Violet 610 SE
NBP1-76755MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-76755-0.025mg
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CRTH-2/GPR44 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human CRTH2. The immunogen is located within the first 50 amino acids of CRTH2. Amino Acid Squence: EQMSRLQSHSNTSIRYID
Specificity
At least two different isoforms of CRTH2 are known to exist; this antibody will detect both isoforms.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PTGDR2
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1 ug/ml
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 25917171).
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
CRTH-2/GPR44 Peptide (NBP1-76755PEP)
Publications
Read Publications using
NBP1-76755 in the following applications:

Reactivity Notes

Guinea Pig reactivity reported in scientific literature (PMID: 25917171).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for CRTH-2/GPR44 Antibody - BSA Free

  • CD294 antigen
  • CD294
  • Chemoattractant receptor-homologous molecule expressed on Th2 cells
  • CRTH2
  • CRTH-2
  • CRTH2chemoattractant receptor homologous molecule expressed on T helper type 2 cells
  • DL1R
  • DP2
  • G protein-coupled receptor 44
  • GPR44
  • PGD2R
  • PTGDR2
  • putative G-protein coupled receptor 44

Background

The chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) is a recently identified receptor for the prostaglandin D2 (PGD2) in addition to the classic prostaglandin D receptor. CRTH2 is expressed on Th2 cells and eosinophils and mediates chemotaxis of these cells to PGD2 and is thus thought to be a key receptor mediating eosinophil and Th2 recruitment during allergic responses. However, CRTH2-null mice showed enhanced eosinophil recruitment into the lung consistent with observations that the CRTH2-null mice produced significantly higher amounts of interleukin-5 (IL-5) and IL-3. This suggests that CRTH2 plays a nonredundant role in restricting eosinophilia and allergic response in vivo. At least two different isoforms of CRTH2 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: BA
CRTH-2/GPR44
Antibody - BSA Free
NBP1-76755
CRTH-2/GPR44 Antibody - BSA Free
Species: Hu, Mu, Rt, Gp
Applications: WB, ELISA, ICC/IF, IHC

Publications for CRTH-2/GPR44 Antibody (NBP1-76755)(4)

We have publications tested in 2 confirmed species: Human, Guinea Pig.

We have publications tested in 4 applications: ICC/IF, IHC-P, MiAr, WB.


Filter By Application
ICC/IF
(2)
IHC-P
(1)
MiAr
(1)
WB
(2)
All Applications
Filter By Species
Human
(1)
Guinea Pig
(2)
All Species

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PTGDR2
Entrez
Uniprot