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gamma Catenin Antibody (2G9)

Images

 
Western Blot: gamma Catenin Antibody (2G9) [H00003728-M01] - Analysis of JUP expression in transfected 293T cell line by JUP monoclonal antibody (M01), clone 2G9.Lane 1: JUP transfected lysate(81.7 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: gamma Catenin Antibody (2G9) [H00003728-M01] - Analysis of monoclonal antibody to JUP on A-431 cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: gamma Catenin Antibody (2G9) [H00003728-M01] - Analysis of monoclonal antibody to JUP on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 3 ug/ml.
Western Blot: gamma Catenin Antibody (2G9) [H00003728-M01] - JUP monoclonal antibody (M01), clone 2G9 Analysis of JUP expression in A-431.
ELISA: gamma Catenin Antibody (2G9) [H00003728-M01] - Detection limit for recombinant GST tagged JUP is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
2G9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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gamma Catenin Antibody (2G9) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
JUP (AAH11865.1, 1 a.a. ~ 745 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEVMNLMEQPIKVTEWQQTYTYDSGIHSGANTCVPSVSSKGIMEEDEACGRQYTLKKTTTYTQGVPPSQGDLEYQMSTTARAKRVREAMCPGVSGEDSSLLLATQVEGQATNLQRLAEPSQLLKSAIVHLINYQDDAELATRALPELTKLLNDEDPVVVTKAAMIVNQLSKKEASRRALMGSPQLVAAVVRTMQNTSDLDTARCTTSILHNLSHHREGLLAIFKSGGIPALVRMLSSPVESVLFYAITTLHNLLLYQEGAKMAVRLADGLQKMVPLLNKNNPKFLAITTDCLQLLAYGNQESKLIILANGGPQALVQIMRNYSYEKLLWTTSRVLKVLSVCPSNKPAIVEAGGMQALGKHLTSNSPRLVQNCLWTLRNLSDVATKQEGLESVLKILVNQLSVDDVNVLTCATGTLSNLTCNNSKNKTLVTQNSGVEALIHAILRAGDKDDITEPAVCALRHLTSRHPEAEMAQNSVRLNYGIPAIVKLLNQPNQWPLVKATIGLIRNLALCPANHAPLQEAAVIPRLVQLLVKAHQDAQRHVAAGTQQPYTDGVRMEEIVEGCTGALHILARDPMNRMEIFRLNTIPLFVQPLYSSVENIQRVAAGALCELAQDKEAADAIDAEGASAPLMELLHSRNEGTATYAAAVLFRISEDKNPDYRKRVSVELTNSLFKHDPAAWEAAQSMIPINEPYGDDLDATYRPMYSSDVPLDPLEMHMDMDGDYPIDTYSDGLRPPYPTADHMLA
Marker
Intercellular Junctions Marker
Specificity
JUP - junction plakoglobin
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
JUP
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.
Publications
Read Publication using H00003728-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for gamma Catenin Antibody (2G9)

  • catenin (cadherin-associated protein), gamma (80kD)
  • catenin (cadherin-associated protein), gamma 80kDa
  • Catenin gamma
  • Desmoplakin III
  • desmoplakin-3
  • DP3CTNNG
  • DPIII
  • gamma-catenin
  • junction plakoglobin
  • PDGBARVD12
  • PKGB

Background

This gene encodes a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. Alternative splicing occurs in this gene; however, not all transcripts have been fully described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00003728-M01
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Publications for gamma Catenin Antibody (H00003728-M01)(1)


Showing Publication 1 - 1 of 1.
Publication using H00003728-M01 Applications Species
Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for gamma Catenin Antibody (H00003728-M01) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for gamma Catenin Antibody (H00003728-M01)

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Blogs on gamma Catenin.

Beta Catenin in Cell Adhesion and T-cell Signaling
Beta Catenin is a cytosolic, 88 kDa intracellular protein that tightly associates with cell surface cadherin glycoproteins. It is one member of the catenin family that includes alpha Catenin, beta Catenin, and gamma Catenin. Colocalization studies usi...  Read full blog post.

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Bioinformatics

Gene Symbol JUP
Entrez
OMIM
Uniprot