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GAD2/GAD65 Antibody

Images

 
Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-89801] - Staining of human skeletal muscle shows no granular cytoplasmic positivity in myocytes as expected.
Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-89801] - Staining of human cerebellum shows moderate granular cytoplasmic positivity in cells in molecular layer.
Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-89801] - Staining of human cerebral cortex shows moderate granular cytoplasmic positivity in neuropil.
Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-89801] - Staining of human pancreas shows strong granular cytoplasmic positivity in islets of Langerhans.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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GAD2/GAD65 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SGFWSFGSEDGSGDSENPGTARAWCQVAQKFTGGIGNKLCALLYGDAEKPAESGGSQPPRAAARKAACACDQKPCSCSKVDVNYAFLHATDLLP
Marker
GABAergic Neuronal Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GAD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
GAD2/GAD65 Protein (NBP1-89801PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (88%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GAD2/GAD65 Antibody

  • EC 4.1.1
  • EC 4.1.1.15
  • GAD2
  • GAD65
  • GAD-65
  • GAD65MGC161605
  • glutamate decarboxylase 2 (pancreatic islets and brain, 65kD)
  • glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
  • glutamate decarboxylase 2
  • Glutamate decarboxylase 65 kDa isoform
  • Glutamate decarboxylase-2 (pancreas)
  • MGC161607,65 kDa glutamic acid decarboxylase

Background

Antibodies to glutamic acid decarboxylase-65 (GAD65) are present in autoimmune disorders such as insulin-dependent (type 1) diabetes mellitus (IDDM), stiff man syndrome and polyendocrine autoimmune disease. Auto-antibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM and thus are important markers for disease activity. These auto-antibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Auto-antibodies to GAD67 are found in only 15% of recent-onset IDDM patients and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GAD2