![Immunohistochemistry: GABA-B R1 Antibody (PSH03-87) [NBP3-32360] - Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-GABA-B R1 antibody (NBP3-32360) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32360) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32360_rabbit-gaba-b-r1-mab-psh03-87-298202414304191.jpg "Immunohistochemistry: GABA-B R1 Antibody (PSH03-87) [NBP3-32360] - Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-GABA-B R1 antibody (NBP3-32360) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32360) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clone | PSH03-87 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein within human GABA-B R1 aa 142-591 / 961. (Uniprot: Q9UBS5) |
| Localization | Cell membrane, Postsynaptic cell membrane, Cell projection, dendrite; Secreted. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | GABBR1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 108 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.1% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | GABBR1 |
![Immunohistochemistry: GABA-B R1 Antibody (PSH03-87) [NBP3-32360] - Immunofluorescence analysis of paraffin-embedded mouse cerebellum tissue labeling GABA-B R1 with Rabbit anti-GABA-B R1 antibody (NBP3-32360) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32360, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32360_rabbit-gaba-b-r1-mab-psh03-87-298202413585541.jpg "Immunohistochemistry: GABA-B R1 Antibody (PSH03-87) [NBP3-32360] - Immunofluorescence analysis of paraffin-embedded mouse cerebellum tissue labeling GABA-B R1 with Rabbit anti-GABA-B R1 antibody (NBP3-32360) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32360, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Western Blot: GABA-B R1 Antibody (PSH03-87) [NBP3-32360] - Western blot analysis of GABA-B R1 on different lysates with Rabbit anti-GABA-B R1 antibody (NBP3-32360) at 1/2,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate (no heat) Lane 4: Mouse cerebellum tissue lysate (70℃ heat) Lane 5: Rat cerebellum tissue lysate Lane 6: Mouse skeletal muscle tissue lysate (no heat) (negative) Lane 7: Rat skeletal muscle tissue lysate (no heat) (negative) Lysates/proteins at 40 µg/Lane. Predicted band size: 108 kDa Observed band size: 95-108 kDa Exposure time: Lane 1-7 (left): 28 seconds; Lane 1-5 (right): 2 minutes 34 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32360_rabbit-gaba-b-r1-mab-psh03-87-3920248453530.jpg "Western Blot: GABA-B R1 Antibody (PSH03-87) [NBP3-32360] - Western blot analysis of GABA-B R1 on different lysates with Rabbit anti-GABA-B R1 antibody (NBP3-32360) at 1/2,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate (no heat) Lane 4: Mouse cerebellum tissue lysate (70℃ heat) Lane 5: Rat cerebellum tissue lysate Lane 6: Mouse skeletal muscle tissue lysate (no heat) (negative) Lane 7: Rat skeletal muscle tissue lysate (no heat) (negative) Lysates/proteins at 40 µg/Lane. Predicted band size: 108 kDa Observed band size: 95-108 kDa Exposure time: Lane 1-7 (left): 28 seconds; Lane 1-5 (right): 2 minutes 34 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
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