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![Western Blot: GABA-B R1 Antibody (2D7) [H00002550-M01] - Analysis of GABBR1 expression in transfected 293T cell line by GABBR1 monoclonal antibody (M01), clone 2D7.Lane 1: GABBR1 transfected lysate(95 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GABA-B-R1-Antibody-2D7-Western-Blot-H00002550-M01-img0016.jpg "Western Blot: GABA-B R1 Antibody (2D7) [H00002550-M01] - Analysis of GABBR1 expression in transfected 293T cell line by GABBR1 monoclonal antibody (M01), clone 2D7.Lane 1: GABBR1 transfected lysate(95 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: GABA-B R1 Antibody (2D7) [H00002550-M01] - Analysis of monoclonal antibody to GABBR1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/GABA-B-R1-Antibody-2D7-Immunocytochemistry-Immunofluorescence-H00002550-M01-img0012.jpg)
![Western Blot: GABA-B R1 Antibody (2D7) [H00002550-M01] - Analysis of GABBR1 over-expressed 293 cell line, cotransfected with GABBR1 Validated Chimera RNAi ( Cat # H00002550-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GABBR1 monoclonal antibody (M01), clone 2D7 (Cat # H00002550-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/GABA-B-R1-Antibody-2D7-Western-Blot-H00002550-M01-img0013.jpg)
![Western Blot: GABA-B R1 Antibody (2D7) [H00002550-M01] - GABBR1 monoclonal antibody (M01), clone 2D7 Analysis of GABBR1 expression in IMR-32.](http://images.novusbio.com/fullsize/GABA-B-R1-Antibody-2D7-Western-Blot-H00002550-M01-img0015.jpg)
![ELISA: GABA-B R1 Antibody (2D7) [H00002550-M01] - Detection limit for recombinant GST tagged GABBR1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GABA-B-R1-Antibody-2D7-ELISA-H00002550-M01-img0014.jpg)
![Western Blot: GABA-B R1 Antibody (S93A-49) [NBP2-59336] - Western Blot analysis of Rat brain membrane lysate showing detection of GABA-B R1 protein using Mouse Anti-GABA-B R1 Monoclonal Antibody, Clone S93A-49 (NBP2-59336). Primary Antibody: Mouse Anti-GABA-B R1 Monoclonal Antibody (NBP2-59336) at 1:1000.](http://images.novusbio.com/fullsize/GABA-B-R1-Antibody-S93A-49-Western-Blot-NBP2-59336-img0005.jpg "Western Blot: GABA-B R1 Antibody (S93A-49) [NBP2-59336] - Western Blot analysis of Rat brain membrane lysate showing detection of GABA-B R1 protein using Mouse Anti-GABA-B R1 Monoclonal Antibody, Clone S93A-49 (NBP2-59336). Primary Antibody: Mouse Anti-GABA-B R1 Monoclonal Antibody (NBP2-59336) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: GABA-B R1 Antibody (S93A-49) [NBP2-59336] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-B Receptor 1 Monoclonal Antibody, Clone S93A-49 (NBP2-59336). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-B Receptor 1 Monoclonal Antibody (NBP2-59336) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-B Receptor 1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/GABA-B-R1-Antibody-S93A-49-Immunocytochemistry-Immunofluorescence-NBP2-59336-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: GABA-B R1 Antibody (S93A-49) [NBP2-59336] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-B Receptor 1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/GABA-B-R1-Antibody-S93A-49-Immunocytochemistry-Immunofluorescence-NBP2-59336-img0002.jpg)
![Immunohistochemistry: GABA-B R1 Antibody (PSH03-87) [NBP3-32360] - Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-GABA-B R1 antibody (NBP3-32360) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32360) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32360_rabbit-gaba-b-r1-mab-psh03-87-298202414304191.jpg "Immunohistochemistry: GABA-B R1 Antibody (PSH03-87) [NBP3-32360] - Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-GABA-B R1 antibody (NBP3-32360) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32360) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: GABA-B R1 Antibody (PSH03-87) [NBP3-32360] - Immunofluorescence analysis of paraffin-embedded mouse cerebellum tissue labeling GABA-B R1 with Rabbit anti-GABA-B R1 antibody (NBP3-32360) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32360, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32360_rabbit-gaba-b-r1-mab-psh03-87-298202413585541.jpg)
![Western Blot: GABA-B R1 Antibody (PSH03-87) [NBP3-32360] - Western blot analysis of GABA-B R1 on different lysates with Rabbit anti-GABA-B R1 antibody (NBP3-32360) at 1/2,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate (no heat) Lane 4: Mouse cerebellum tissue lysate (70℃ heat) Lane 5: Rat cerebellum tissue lysate Lane 6: Mouse skeletal muscle tissue lysate (no heat) (negative) Lane 7: Rat skeletal muscle tissue lysate (no heat) (negative) Lysates/proteins at 40 µg/Lane. Predicted band size: 108 kDa Observed band size: 95-108 kDa Exposure time: Lane 1-7 (left): 28 seconds; Lane 1-5 (right): 2 minutes 34 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32360_rabbit-gaba-b-r1-mab-psh03-87-3920248453530.jpg)
![Immunohistochemistry-Paraffin: GABA-B R1 Antibody [NLS5332] - Anti-GABABR1 / GABBR1 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GABA-B-R1-Antibody-Immunohistochemistry-Paraffin-NLS5332-img0003.jpg "Immunohistochemistry-Paraffin: GABA-B R1 Antibody [NLS5332] - Anti-GABABR1 / GABBR1 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: GABA-B R1 Antibody [NLS5332] - GABA B Receptor 1 Antibody [NLS5332] - IHC analysis of Brain, Neurons and glia using NLS5332 at 7 ug/ ml.](http://images.novusbio.com/fullsize/GABA-B-R1-Antibody-Immunohistochemistry-Paraffin-NLS5332-img0002.jpg)
![Immunohistochemistry-Paraffin: GABA-B R1 Antibody [NBP1-52389] - Analysis of anti-GABBR1 antibody with human brain, cortex at concentration 3.75 ug/ml.](http://images.novusbio.com/fullsize/GABA-B-R1-Antibody-Immunohistochemistry-Paraffin-NBP1-52389-img0002.jpg "Immunohistochemistry-Paraffin: GABA-B R1 Antibody [NBP1-52389] - Analysis of anti-GABBR1 antibody with human brain, cortex at concentration 3.75 ug/ml.")
![Western Blot: GABA-B R1 Antibody [NB300-160] - Brain lysate with GABA-B R1 antibody at a dilution of 1:500.](http://images.novusbio.com/fullsize/GABA-B-R1-Antibody-Western-Blot-NB300-160-img0003.jpg "Western Blot: GABA-B R1 Antibody [NB300-160] - Brain lysate with GABA-B R1 antibody at a dilution of 1:500.")
![Western Blot: GABA-B R1 Antibody (JE60-09) [NBP3-32359] - Western blot analysis of GABA-B R1 on different lysates with Rabbit anti-GABA-B R1 antibody (NBP3-32359) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: Jurkat cell lysate Lane 3: HeLa cell lysate Lane 4: SHG-44 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 108 kDa Observed band size: 108 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32359) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32359_rabbit-gaba-b-r1-mab-je60-09-278202415212436.jpg "Western Blot: GABA-B R1 Antibody (JE60-09) [NBP3-32359] - Western blot analysis of GABA-B R1 on different lysates with Rabbit anti-GABA-B R1 antibody (NBP3-32359) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: Jurkat cell lysate Lane 3: HeLa cell lysate Lane 4: SHG-44 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 108 kDa Observed band size: 108 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32359) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: GABA-B R1 Antibody (JE60-09) [NBP3-32359] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GABA-B R1 antibody (NBP3-32359) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32359) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32359_rabbit-gaba-b-r1-mab-je60-09-298202414333648.jpg)
![Western Blot: GABA-B R1 Antibody (SR1045) [NBP3-21802] - Western blot analysis using NBP3-21802 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21802_rabbit-gaba-b-r1-mab-sr1045-57202316582189.jpg "Western Blot: GABA-B R1 Antibody (SR1045) [NBP3-21802] - Western blot analysis using NBP3-21802 in HeLa cell lysate.")
![Western Blot: GABA-B R1 [p Ser923] Antibody [NB100-61102] - Western Blot: GABA-B R1 [p Ser923] Antibody [NB100-61102] - Rat synaptic membrane showing specific immune labeling of the ~102k GABA-B R1 [pt Ser923[ (control). The phosphor specificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to theto the p-Ser923 GABA-B antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/GABA-B-R1-[p-Ser923]-Antibody-Western-Blot-NB100-61102-img0006.jpg "Western Blot: GABA-B R1 [p Ser923] Antibody [NB100-61102] - Western Blot: GABA-B R1 [p Ser923] Antibody [NB100-61102] - Rat synaptic membrane showing specific immune labeling of the ~102k GABA-B R1 [pt Ser923[ (control). The phosphor specificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to theto the p-Ser923 GABA-B antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.")
![SDS-Page: Recombinant Human GABA-B R1 Protein [H00002550-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00002550-Q01-1.jpg "SDS-Page: Recombinant Human GABA-B R1 Protein [H00002550-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")