![Western Blot: FUBP1 Antibody [NBP2-16542] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FUBP1-Antibody-Western-Blot-NBP2-16542-img0012.jpg "Western Blot: FUBP1 Antibody [NBP2-16542] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Bv, Ch, XpSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human FUBP1. The exact sequence is proprietary. |
| Localization | Nucleus |
| Marker | Nucleus without Nucleoli Marker |
| Predicted Species | Bovine (99%), Xenopus (92%), Chicken (94%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | FUBP1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 68 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: FUBP1 Antibody [NBP2-16542] - Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FUBP1 protein stained by FUBP1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/FUBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16542-img0006.jpg "Immunocytochemistry/Immunofluorescence: FUBP1 Antibody [NBP2-16542] - Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FUBP1 protein stained by FUBP1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP2-16542] - Mouse testis. FUBP1 antibody dilution: 1:1000. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/FUBP1-Antibody-Immunohistochemistry-Paraffin-NBP2-16542-img0005.jpg "Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP2-16542] - Mouse testis. FUBP1 antibody dilution: 1:1000. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: FUBP1 Antibody [NBP2-16542] - 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of FUBP-1 protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of FUBP-1 by 2.5 ug of FUBP-1 antibody 7.5% SDS-PAGE The immunoprecipitated FUBP-1 protein was detected by FUBP-1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/FUBP1-Antibody-Western-Blot-NBP2-16542-img0003.jpg "Western Blot: FUBP1 Antibody [NBP2-16542] - 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of FUBP-1 protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of FUBP-1 by 2.5 ug of FUBP-1 antibody 7.5% SDS-PAGE The immunoprecipitated FUBP-1 protein was detected by FUBP-1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.")
![Western Blot: FUBP1 Antibody [NBP2-16542] - Hela whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FUBP1-Antibody-Western-Blot-NBP2-16542-img0008.jpg "Western Blot: FUBP1 Antibody [NBP2-16542] - Hela whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: FUBP1 Antibody [NBP2-16542] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FUBP1-Antibody-Western-Blot-NBP2-16542-img0010.jpg "Western Blot: FUBP1 Antibody [NBP2-16542] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP2-16542] - Mouse testis. FUBP1 antibody dilution: 1:1000. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/FUBP1-Antibody-Immunohistochemistry-Paraffin-NBP2-16542-img0004.jpg "Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP2-16542] - Mouse testis. FUBP1 antibody dilution: 1:1000. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")