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PUF60 Antibody

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Western Blot: PUF60 Antibody [NBP2-49303] - Analysis using Anti-PUF60 antibody NBP2-49303 (A) shows similar pattern to independent antibody NBP2-49032 (B).
Independent Antibodies: Western Blot: PUF60 Antibody [NBP2-49303] - Analysis using Anti-PUF60 antibody NBP2-49303 (A) shows similar pattern to independent antibody NBP2-49032 (B).
Immunocytochemistry/ Immunofluorescence: PUF60 Antibody [NBP2-49303] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49303] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49303] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49303] - Staining of human testis shows high expression.
Orthogonal Strategies: Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49303] - Staining in human testis and pancreas tissues using anti-PUF60 antibody. Corresponding PUF60 RNA-seq data are presented for the ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49303] - Staining of human cerebral cortex, colon, kidney and testis using Anti-PUF60 antibody NBP2-49303 (A) shows similar protein ...read more
Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49303] - Staining of human colon.
Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49303] - Staining of human kidney.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

      

Orthogonal Strategies

  

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PUF60 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: PIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGG
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PUF60
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PUF60 Recombinant Protein Antigen (NBP2-49303PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PUF60 Antibody

  • FBP interacting repressor
  • FBP-interacting repressor
  • FIRpoly-U binding splicing factor PUF60
  • FLJ31379
  • FUSE-binding protein-interacting repressor
  • poly(U)-binding-splicing factor PUF60
  • poly-U binding splicing factor 60KDa
  • Ro ribonucleoprotein binding protein 1
  • Ro ribonucleoprotein-binding protein 1
  • Ro-binding protein 1
  • roBP1
  • RoBPI
  • siah binding protein 1,60 kDa poly(U)-binding-splicing factor
  • Siah-binding protein 1
  • siah-BP1
  • SIAHBP1pyrimidine tract binding splicing factor

Background

PUF60 is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. PUF60 also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. PUF60 gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for PUF60 gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PUF60