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FUBP1 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918] - Staining in human lymph node and skeletal muscle tissues.. Corresponding FUBP1 RNA-seq data are presented for the same tissues.
Genetic Strategies: Western Blot: FUBP1 Antibody [NBP1-83918] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: ...read more
Immunocytochemistry/ Immunofluorescence: FUBP1 Antibody [NBP1-83918] - Staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918] - Staining of human skeletal muscle shows strong nuclear positivity in myocytes.
Western Blot: FUBP1 Antibody [NBP1-83918] - Analysis in human cell line SH-SY5Y.
Western Blot: FUBP1 Antibody [NBP1-83918] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918] - Staining of human lymph node shows strong nuclear positivity in germinal center cells.
Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918] - Staining of human endometrium shows strong nuclear positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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FUBP1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GGGGVNDAFKDALQRARQIAAKIGGDAGTSLNSNDYGYGGQKRPLEDGDQPDAKKVAPQNDSFGTQLPPMHQQQRSVMTEEYKVPDGMVGFIIGRGGEQISRIQQESGCKIQIAPDSGGLPERSCML
Marker
Nucleus without Nucleoli Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FUBP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
FUBP1 Protein (NBP1-83918PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for FUBP1 Antibody

  • far upstream element (FUSE) binding protein 1
  • far upstream element binding protein
  • far upstream element-binding protein 1
  • FBPDNA helicase V
  • FUBP
  • FUSE-binding protein 1
  • hDH V

Background

FUBP1 encodes a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FUBP1 Antibody (NBP1-83918) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FUBP1