![Western Blot: SSB Antibody (OTI2C8) [NBP1-48002] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SSB monoclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Western-Blot-NBP1-48002-img0015.jpg "Western Blot: SSB Antibody (OTI2C8) [NBP1-48002] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SSB monoclonal antibody.")
| Reactivity | Hu, Ca, Pm, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clone | OTI2C8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.8 mg/ml |
| Immunogen | Full-length protein expressed in 293T cell transfected with human SSB expression vector |
| Specificity | This antibody is specific for Homo sapiens Sjogren syndrome antigen B (autoantigen La) (SSB). |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SSB |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IP was tested using 2ug of antibody per 500ul at 0.5mg/ml overexpression lysate. Optimal dilutions for other samples will need to be determined by the end user. |
| Theoretical MW | 46.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.8 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for SSB Antibody (NBP1-48002)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SSB.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunocytochemistry-Immunofluorescence-NBP1-48002-img0013.jpg "Immunocytochemistry/Immunofluorescence: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SSB.")
![Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human prostate tissue using anti-SSB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunohistochemistry-Paraffin-NBP1-48002-img0012.jpg "Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human prostate tissue using anti-SSB mouse monoclonal antibody.")
![Western Blot: SSB Antibody (OTI2C8) [NBP1-48002] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Western-Blot-NBP1-48002-img0001.jpg "Western Blot: SSB Antibody (OTI2C8) [NBP1-48002] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB.")
![Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SSB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunohistochemistry-Paraffin-NBP1-48002-img0002.jpg "Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SSB mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SSB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunohistochemistry-Paraffin-NBP1-48002-img0003.jpg "Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SSB mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SSB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunohistochemistry-Paraffin-NBP1-48002-img0004.jpg "Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SSB mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human breast tissue using anti-SSB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunohistochemistry-Paraffin-NBP1-48002-img0005.jpg "Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human breast tissue using anti-SSB mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human colon tissue using anti-SSB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunohistochemistry-Paraffin-NBP1-48002-img0006.jpg "Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human colon tissue using anti-SSB mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human endometrium tissue using anti-SSB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunohistochemistry-Paraffin-NBP1-48002-img0007.jpg "Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human endometrium tissue using anti-SSB mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human Kidney tissue using anti-SSB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunohistochemistry-Paraffin-NBP1-48002-img0008.jpg "Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human Kidney tissue using anti-SSB mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human lung tissue using anti-SSB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunohistochemistry-Paraffin-NBP1-48002-img0009.jpg "Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human lung tissue using anti-SSB mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human Ovary tissue using anti-SSB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunohistochemistry-Paraffin-NBP1-48002-img0010.jpg "Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human Ovary tissue using anti-SSB mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human pancreas tissue using anti-SSB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunohistochemistry-Paraffin-NBP1-48002-img0011.jpg "Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human pancreas tissue using anti-SSB mouse monoclonal antibody.")
![Immunoprecipitation: SSB Antibody (OTI2C8) [NBP1-48002] - (Negative control: IP without adding anti-SSB antibody.). For each experiment, 500ul of DDK tagged SSB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SSB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/SSB-Antibody-OTI2C8-Immunoprecipitation-NBP1-48002-img0016.jpg "Immunoprecipitation: SSB Antibody (OTI2C8) [NBP1-48002] - (Negative control: IP without adding anti-SSB antibody.). For each experiment, 500ul of DDK tagged SSB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SSB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")