SSB Antibody (OTI2C8)

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Western Blot: SSB Antibody (OTI2C8) [NBP1-48002] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SSB monoclonal antibody.

Product Details

Summary
Reactivity Hu, Ca, Pm, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
OTI2C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.8 mg/ml

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SSB Antibody (OTI2C8) Summary

Immunogen
Full-length protein expressed in 293T cell transfected with human SSB expression vector
Specificity
This antibody is specific for Homo sapiens Sjogren syndrome antigen B (autoantigen La) (SSB).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SSB
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Immunoprecipitation 2ug/500ul
  • Western Blot 1:1000-2000
Application Notes
IP was tested using 2ug of antibody per 500ul at 0.5mg/ml overexpression lysate. Optimal dilutions for other samples will need to be determined by the end user.
Theoretical MW
46.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.8 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SSB Antibody (OTI2C8)

  • La autoantigen
  • La ribonucleoprotein
  • lupus La protein
  • member 3
  • Sjoegren syndrome type B antigen
  • Sjogren syndrome antigen B (autoantigen La)
  • SS-B
  • SS-B/La protein

Background

La is involved in diverse aspects of RNA metabolism, including binding and protecting 3-prime UUU(H) elements of newly RNA polymerase III (see MIM 606007)-transcribed RNA, processing 5-prime and 3-prime ends of pre-tRNA precursors, acting as an RNA chaper

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SSB
Entrez