![Immunoprecipitation: FUBP1 Antibody (7C3) [NBP3-26564] - Immunoprecipitating FUBP1 in Jurkat whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26564 in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26564 (2ug) + Jurkat whole cell lysate (500ug). Lane 3: Jurkat whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26564_rabbit-fubp1-mab-7c3-2622024132376.jpg "Immunoprecipitation: FUBP1 Antibody (7C3) [NBP3-26564] - Immunoprecipitating FUBP1 in Jurkat whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26564 in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26564 (2ug) + Jurkat whole cell lysate (500ug). Lane 3: Jurkat whole cell lysate (10ug).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, IP |
| Clone | 7C3 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant monoclonal antibody expressed in HEK293F cells |
| Immunogen | A synthesized peptide derived from Human FUBP1 [UniProt Q96AE4] |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | FUBP1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20 to -70C. Avoid freeze-thaw cycles. |
| Buffer | PBS, pH 7.4, 150mM NaCl, and 50% glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
![Flow Cytometry FOLR1 Antibody (548908) [Unconjugated]](https://images.novusbio.com/images2/FOLR1_MAB5646_Flow_Cytometry_10781.jpg)
![Western Blot FOLR1 Antibody (548908) [Unconjugated]](https://images.novusbio.com/images2/FOLR1_MAB5646_Western_Blot_6248.jpg)
![Immunocytochemistry FOLR1 Antibody (548908) [Unconjugated]](https://images.novusbio.com/images2/FOLR1_MAB5646_Immunocytochemistry_6624.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for FUBP1 Antibody (NBP3-26564)Find related products by research area.
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| Gene Symbol | FUBP1 |
![Immunohistochemistry: FUBP1 Antibody (7C3) [NBP3-26564] - Image of FUBP1 Antibody (7C3) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26564_rabbit-fubp1-mab-7c3-26220241535570.jpg "Immunohistochemistry: FUBP1 Antibody (7C3) [NBP3-26564] - Image of FUBP1 Antibody (7C3) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: FUBP1 Antibody (7C3) [NBP3-26564] - Image of FUBP1 Antibody (7C3) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26564_rabbit-fubp1-mab-7c3-262202415355746.jpg "Immunohistochemistry: FUBP1 Antibody (7C3) [NBP3-26564] - Image of FUBP1 Antibody (7C3) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: FUBP1 Antibody (7C3) [NBP3-26564] - Positive Western Blot detected in: Jurkat whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate. All lanes: FUBP1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 68, 69 kDa Observed band size: 69 kDa](http://images.novusbio.com/fullsize/nbp3-26564_rabbit-fubp1-mab-7c3-282202411442642.jpg "Western Blot: FUBP1 Antibody (7C3) [NBP3-26564] - Positive Western Blot detected in: Jurkat whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate. All lanes: FUBP1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 68, 69 kDa Observed band size: 69 kDa")
![Immunocytochemistry/Immunofluorescence: FUBP1 Antibody (7C3) [NBP3-26564] - Staining of Hela Cells with FUBP1 Antibody (7C3) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26564_rabbit-fubp1-mab-7c3-282202412475147.jpg "Immunocytochemistry/Immunofluorescence: FUBP1 Antibody (7C3) [NBP3-26564] - Staining of Hela Cells with FUBP1 Antibody (7C3) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).")
![Flow Cytometry: FUBP1 Antibody (7C3) [NBP3-26564] - Overlay histogram showing Jurkat cells stained with FUBP1 Antibody (7C3) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26564_rabbit-fubp1-mab-7c3-282202414312710.jpg "Flow Cytometry: FUBP1 Antibody (7C3) [NBP3-26564] - Overlay histogram showing Jurkat cells stained with FUBP1 Antibody (7C3) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.")
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![Knockout Validated Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Knockout_Validated_24032.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")