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FoxP3 Antibody (1054C) [Unconjugated]

 (19 Publications)
 (1 Review)
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FOXP3 was detected in immersion fixed paraffin-embedded sections of Hodgkin's Lymphoma using Rabbit Anti-Human FOXP3 Monoclonal Antibody (Catalog # MAB8214) at 20 µg/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the nucleus. Protocol available in COMET™ Panel Builder.
FOXP3 was detected in immersion fixed paraffin-embedded sections of Hodgkin's Lymphoma using Rabbit Anti-Human FOXP3 Monoclonal Antibody (Catalog # MAB8214) at 20 µg/mL at 37 ° Celsius for 4 minutes. Before incubation ...read more
FOXP3 Antibody was detected in immersion fixed paraffin-embedded sections of mouse Thymus using Rabbit Anti-Mouse FOXP3 Monoclonal Antibody (Catalog # MAB8214) at 20ug/mL at 37 ° Celsius for 4 minutes. Before ...read more
Biological Strategies: Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) stimulated to induce Regulatory T Cells (Tregs) with Recombinant Human TGF-beta 1 (Catalog # 240-B) and ...read more
Mouse splenocytes were stained with Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A) and either (A) Rabbit Anti-Human/Mouse FoxP3 Monoclonal Antibody (Catalog # MAB8214) or (B) Normal Rabbit IgG ...read more
FoxP3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human/Mouse FoxP3 Monoclonal Antibody (Catalog # MAB8214) at 8 µg/mL for 3 hours at room temperature. Cells ...read more
FoxP3 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human/Mouse FoxP3 Monoclonal Antibody (Catalog # MAB8214) at 15 µg/mL overnight at 4 °C. Tissue was stained using the ...read more
FoxP3 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Rabbit Anti-Human/Mouse FoxP3 Monoclonal Antibody (Catalog # MAB8214) at 5 µg/mL for 1 hour at room temperature ...read more
Orthogonal Strategies: FoxP3 mRNA (red) and protein (green) was detected in formalin-fixed paraffin-embedded tissue sections of human tonsil probed with ACD RNAScope®Probe (Catalog # 418471) followed by ...read more
Multiplex fluorescent immunohistochemistry staining. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31192136), licensed under a CC-BY license. Not internally tested by ...read more
Multiplex fluorescent immunohistochemistry staining. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31192136), licensed under a CC-BY license. Not internally tested by ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow, IHC, Dual ISH-IHC, ICC/IF, mIF
Clone
1054C
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

    

Orthogonal Strategies

  

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC8214U-100UG
Alexa Fluor 405
IC8214V-100UG
Alexa Fluor 488
IC8214G-100
Alexa Fluor 532
FAB8214X
Alexa Fluor 594
IC8214T-100UG
Alexa Fluor 647
IC8214R-100UG
Alexa Fluor 700
IC8214N-100
Alexa Fluor 750
IC8214S-100UG
Allophycocyanin
IC8214A-100
Allophycocyanin
FPK8214A-100
Allophycocyanin
FPK8214A-025
Allophycocyanin/Cy7
FAB8214APCCY7
Biotin
FAB8214B
CoraFluor 1
FAB8214CL1
DyLight 350
FAB8214D
DyLight 405
FAB8214E
DyLight 488
FAB8214K
DyLight 550
FAB8214L
DyLight 594
FAB8214M
DyLight 650
FAB8214W
DyLight 680
FAB8214Y
DyLight 755
FAB8214Z
FITC
FAB8214F
HRP
FAB8214H
Janelia Fluor 525
FAB8214JF525
Janelia Fluor 549
FAB8214I
Janelia Fluor 585
FAB8214JF585
Janelia Fluor 635
FAB8214JF635
Janelia Fluor 646
FAB8214J
Janelia Fluor 669
FAB8214JF669
PE/Atto594
FAB8214PEATT594
PE/Cy5.5
FAB8214PECY55
PE/Cy7
FAB8214PECY7
PerCP
FAB8214C
Phycoerythrin
FPK8214P-100
Phycoerythrin
FPK8214P-025
Phycoerythrin
IC8214P-100
mFluor Violet 450 SE
FAB8214MFV450
mFluor Violet 500 SE
FAB8214MFV500
mFluor Violet 610 SE
FAB8214MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
IC8214P-100
Carrier Free
IC8214A-100
Carrier Free
IC8214G-100
Carrier Free
IC8214N-100
Carrier Free
FPK8214A-025
Carrier Free
FPK8214A-100
Carrier Free
FPK8214P-025
Carrier Free
FPK8214P-100
Carrier Free
MAB8214-SP
Carrier Free
IC8214V-100UG
Carrier Free
IC8214U-100UG
Carrier Free
IC8214T-100UG
Carrier Free
IC8214S-100UG
Carrier Free
IC8214R-100UG
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FoxP3 Antibody (1054C) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
E. coli-derived recombinant human FoxP3
Met1-Leu71
Accession # Q9BZS1
Specificity
Detects human FoxP3 in direct ELISAs. Detects human and mouse FoxP3 in flow cytometry.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
FOXP3
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Dual RNAscope ISH-IHC 5-25 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Multiplex Immunofluorescence 20 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB8214 in the following applications:
Publications
Read Publications using
MAB8214 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
Sodium Azide
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FoxP3 Antibody (1054C) [Unconjugated]

  • AIID
  • AIIDMGC141961
  • DIETER
  • forkhead box P3
  • Forkhead Box Protein P3
  • FoxP3
  • FOXP3delta7
  • immune dysregulation, polyendocrinopathy, enteropathy, X-linked
  • Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked
  • IPEX
  • JM2
  • MGC141961
  • MGC141963
  • PIDX
  • PIDXMGC141963
  • SCURFIN
  • XPID
  • XPIDpolyendocrinopathy, enteropathy, X-linked

Background

Human FoxP3 is a 47 kDa member of the P subclass of the FOX (forkhead box) family of transcription factors. It contains a Leu-rich repeat, a C2H2 zinc finger region, and a C-terminal FKH (fork head), DNA-binding domain. Three isoforms for FoxP3 have been reported. All three isoforms share the sequence used as the immunogen. FoxP3 directly associates with NFAT and NFkB, suppressing their activity in CD4+ T cells. In human, FoxP3 is found in CD4+, CD8+ and CD4+CD25+ T cells. Over the region used for immunization of the amino acid sequence, mouse FoxP3 is 83% to 88% identical to rat, human, canine, and bovine FoxP3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FoxP3 Antibody (MAB8214)(19)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC, IHC-P.


Filter By Application
IHC
(7)
IHC-P
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(6)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using MAB8214 Applications Species
L Sui, S Wang, D Ganguly, TP El Rayes, C Askeland, A Børretzen, D Sim, OJ Halvorsen, G Knutsvik, J Arnes, S Aziz, S Haukaas, WD Foulkes, DR Bielenberg, A Ziemys, V Mittal, RA Brekken, LA Akslen, RS Watnick PRSS2 remodels the tumor microenvironment via repression of Tsp1 to stimulate tumor growth and progression Nature Communications, 2022-12-27;13(1):7959. 2022-12-27 [PMID: 36575174] (IHC, Mouse) IHC Mouse
Y Xu, Z Wei, M Feng, D Zhu, S Mei, Z Wu, Q Feng, W Chang, M Ji, C Liu, Y Zhu, L Shen, F Yang, Y Chen, Y Feng, J Xu, D Zhu Tumor-infiltrated activated B cells suppress liver metastasis of colorectal cancers Cell Reports, 2022-08-30;40(9):111295. 2022-08-30 [PMID: 36044847] (IHC, Mouse) IHC Mouse
Lidia Yshii, Emanuela Pasciuto, Pascal Bielefeld, Loriana Mascali, Pierre Lemaitre, Marika Marino et al. Astrocyte-targeted gene delivery of interleukin 2 specifically increases brain-resident regulatory T cell numbers and protects against pathological neuroinflammation Nature Immunology 6/1/2022 [PMID: 35618831]
Huocong Huang, Zhaoning Wang, Yuqing Zhang, Rachana N. Pradhan, Debolina Ganguly, Raghav Chandra et al. Mesothelial cell-derived antigen-presenting cancer-associated fibroblasts induce expansion of regulatory T cells in pancreatic cancer Cancer Cell 6/1/2022 [PMID: 35523176]
Lisha Zhou, Meiling Wang, Hanrui Guo, Jun Hou, Yingna Zhang, Man Li et al. Integrated Analysis Highlights the Immunosuppressive Role of TREM2+ Macrophages in Hepatocellular Carcinoma Frontiers in Immunology 3/14/2022 [PMID: 35359989]
Gyu Jin Lee, Yukyung Jun, Yoon Kyung Jeon, Daekee Lee, Sanghyuk Lee, Jaesang Kim Mice transgenic for human CTLA4-CD28 fusion gene show proliferation and transformation of ATLL-like and AITL-like T cells OncoImmunology 12/31/2022 [PMID: 35003894]
Y Zhang, H Huang, M Coleman, A Ziemys, P Gopal, SM Kazmi, RA Brekken VEGFR2 activity on myeloid cells mediates immune suppression in the tumor microenvironment JCI Insight, 2021-12-08;6(23):. 2021-12-08 [PMID: 34673569] (IHC, Mouse) IHC Mouse
YF Sun, L Wu, SP Liu, MM Jiang, B Hu, KQ Zhou, W Guo, Y Xu, Y Zhong, XR Zhou, ZF Zhang, G Liu, S Liu, YH Shi, Y Ji, M Du, NN Li, GB Li, ZK Zhao, XY Huang, LQ Xu, QC Yu, DH Peng, SJ Qiu, HC Sun, M Dean, XD Wang, WY Chung, AR Dennison, J Zhou, Y Hou, J Fan, XR Yang Dissecting spatial heterogeneity and the immune-evasion mechanism of CTCs by single-cell RNA-seq in hepatocellular carcinoma Nature Communications, 2021-07-02;12(1):4091. 2021-07-02 [PMID: 34215748] (IHC, Human) IHC Human
Ryuhei Okada, Aki Furusawa, Daniel W. Vermeer, Fuyuki Inagaki, Hiroaki Wakiyama, Takuya Kato et al. Near-infrared photoimmunotherapy targeting human-EGFR in a mouse tumor model simulating current and future clinical trials EBioMedicine 5/1/2021 [PMID: 33933782]
Choi YW, Kim YH, Oh SY et al. Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer Advanced Science 1/4/2021 [PMID: 33643790]
Show All 19 Publications.

Review for FoxP3 Antibody (MAB8214) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB8214:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin FoxP3 MAB8214
Enlarge 		5
reviewed by:
Manuel Flores
 IHC-P Human 03/03/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedFFPE human tonsil,FFPE Tonsil
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for FoxP3 Antibody (MAB8214). (Showing 1 - 3 of 3 FAQs).

  1. Does FoxP3 antibodies comes in lyophilized form?
    • FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.
  2. What the theoretical molecular weight for FoxP3 antibodies?
    • The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.
  3. What is the immunogen sequence of this FoxP3 antibody?
    • A sequence from Met1 - Leu71.

Secondary Antibodies

 

Isotype Controls

Additional FoxP3 Products

Blogs on FoxP3.

Check out the latest blog posts on FoxP3.
Meeting Report: 2nd International Antibody Validation Meeting
Bio-Techne brands Novus Biologicals® and R&D Systems® were proud to support the 2nd International Antibody Validation Meeting held at Bath University, on the 15-16 September, 2016. Almost 100 participants from around the world, including funde...  Read full blog post.
FOXP3
Is has been established that the regulatory transcription factor FOXP3 (a member of the forkhead/winged-helix family of transcription factors) is imperative to immune system homeostasis through CD4+CD25+ regulatory T cell function.  Distinctively, ...  Read full blog post.
FOXP3 is a Master Regulator of T Regulatory (Treg) Cells
FOXP3, a member of forkhead/winged-helix family of transcription factors acts as a "master" regulator for the development and suppressive function of regulatory T cells (Tregs). Its constitutive expression is necessary for the suppressive function of ...  Read full blog post.
FOXP3: Master Regulatory Transcriptional Factor
FOXP3, a forkhead family transcription factor specially expressed in regulatory T (Treg) cells, controls the expression of many key immune-regulatory genes. Treg cells are a population of T lymphocytes that have critical roles in the immune system hom...  Read full blog post.
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Immunohistochemistry-Paraffin FoxP3 MAB8214
Enlarge
 Manuel Flores
03/03/2017
Application: IHC-P
Species: Human
read this review

Bioinformatics

Gene Symbol FOXP3
Uniprot