Home » FoxP3 » FoxP3 Antibodies » FoxP3 Antibody (1054C) [Allophycocyanin]
Novus Biologicals products are now on bio-techne.com

FoxP3 Antibody (1054C) [Allophycocyanin]

 Submit a Review

Images

 
(
Enlarge)
Human peripheral blood mononuclear cell (PBMC) natural regulatory T cells (Tregs) were surface stained with (A) Mouse Anti-Human CD4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3791F) and (B) Mouse Anti-Human IL-2 R alpha (CD25) PE-conjugated Monoclonal Antibody (Catalog # FAB1020P), followed by intracellular staining using Rabbit Anti-Human/Mouse FoxP3 APC-conjugated Monoclonal Antibody (Catalog # IC8214A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Cells were gated on lymphocytes
Human peripheral blood mononuclear cell (PBMC) natural regulatory T cells (Tregs) were surface stained with (A) Mouse Anti-Human CD4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3791F) and (B) Mouse ...read more
C57BL/6 mouse splenocyte natural regulatory T cells (Tregs) were surface stained with (A) Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and (B) Rat Anti-Mouse IL-2 R alpha (CD25) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow
Clone
1054C
Clonality
Monoclonal
Host
Rabbit
Conjugate
Allophycocyanin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC8214U-100UG
Alexa Fluor 405
IC8214V-100UG
Alexa Fluor 488
IC8214G-100
Alexa Fluor 532
FAB8214X
Alexa Fluor 594
IC8214T-100UG
Alexa Fluor 647
IC8214R-100UG
Alexa Fluor 700
IC8214N-100
Alexa Fluor 750
IC8214S-100UG
Allophycocyanin
IC8214A-100
Allophycocyanin
FPK8214A-100
Allophycocyanin/Cy7
FAB8214APCCY7
Biotin
FAB8214B
DyLight 350
FAB8214D
DyLight 405
FAB8214E
DyLight 488
FAB8214K
DyLight 550
FAB8214L
DyLight 594
FAB8214M
DyLight 650
FAB8214W
DyLight 680
FAB8214Y
DyLight 755
FAB8214Z
FITC
FAB8214F
HRP
FAB8214H
Janelia Fluor 549
FAB8214I
Janelia Fluor 646
FAB8214J
PE/Atto594
FAB8214PEATT594
PE/Cy5.5
FAB8214PECY55
PE/Cy7
FAB8214PECY7
PerCP
FAB8214C
Phycoerythrin
FPK8214P-100
Phycoerythrin
FPK8214P-025
Phycoerythrin
IC8214P-100
Unconjugated
MAB8214
mFluor Violet 450 SE
FAB8214MFV450
mFluor Violet 500 SE
FAB8214MFV500
mFluor Violet 610 SE
FAB8214MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB8214
Carrier Free
IC8214P-100
Carrier Free
IC8214A-100
Carrier Free
IC8214G-100
Carrier Free
IC8214N-100
Carrier Free
FPK8214A-100
Carrier Free
FPK8214P-025
Carrier Free
FPK8214P-100
Carrier Free
MAB8214
Carrier Free
MAB8214-SP
Carrier Free
IC8214V-100UG
Carrier Free
IC8214U-100UG
Carrier Free
IC8214T-100UG
Carrier Free
IC8214S-100UG
Carrier Free
IC8214R-100UG
100% Guarantee on every product

FoxP3 Antibody (1054C) [Allophycocyanin] Summary

Additional Information
Recombinant Monoclonal Antibody. A flow cytometry staining kit containing an APC-conjugated FoxP3 monoclonal antibody (IC8214A) and FoxP3 Fixation and Permeabilization Buffers (FC012)
Immunogen
E. coli-derived recombinant human FoxP3
Met1-Leu71
Specificity
Detects human and mouse FoxP3 in flow cytometry.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
FOXP3
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 to 8 °C. Do not use past expiration date. Protect from light.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Kit Components

Components
  1. FC012-025: 8 mL of FoxP3 Fixation Concentrate (4X); 25 mL of FoxP3 Fixation Diluent; 25 mL of FoxP3 Permeabilization and Wash Buffer (10X)
  2. IC8214A-025: 250 ul APC-conjugated antibody specific for human/mouse FoxP3 in a bufferedprotein base with preservatives

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FoxP3 Antibody (1054C) [Allophycocyanin]

  • AIID
  • AIIDMGC141961
  • DIETER
  • forkhead box P3
  • Forkhead Box Protein P3
  • FoxP3
  • FOXP3delta7
  • immune dysregulation, polyendocrinopathy, enteropathy, X-linked
  • Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked
  • IPEX
  • JM2
  • MGC141961
  • MGC141963
  • PIDX
  • PIDXMGC141963
  • SCURFIN
  • XPID
  • XPIDpolyendocrinopathy, enteropathy, X-linked

Background

Human FoxP3 is a 47 kDa member of the P subclass of the FOX (forkhead box) family of transcription factors. It contains a Leu-rich repeat, a C2H2 zinc finger region, and a C-terminal FKH (fork head), DNA-binding domain. Three isoforms for FoxP3 have been reported. All three isoforms share the sequence used as the immunogen. FoxP3 directly associates with NFAT and NFkB, suppressing their activity in CD4+ T cells. In human, FoxP3 is found in CD4+, CD8+ and CD4+CD25+ T cells. Over the region used for immunization of the amino acid sequence, mouse FoxP3 is 83% to 88% identical to rat, human, canine, and bovine FoxP3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

CD4 Antibody - BSA
Free
NBP1-19371
Immunohistochemistry-Frozen CD4 Antibody - BSA Free
Immunohistochemistry-Paraffin CD4 Antibody - BSA Free
Flow Cytometry CD4 Antibody - BSA Free
(9 Reviews)
(49 Publications)
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
CD25/IL-2R alpha
[HRP]
DR2A00
N/A CD25/IL-2R alpha [HRP]
N/A CD25/IL-2R alpha [HRP]
(36 Publications)
Species: Hu
Applications: ELISA
ISG20 Antibody
(1B2-3C9)
H00003669-M01
Western Blot ISG20 Antibody (1B2-3C9)
Western Blot ISG20 Antibody (1B2-3C9)
Immunocytochemistry/ Immunofluorescence ISG20 Antibody (1B2-3C9)
(1 Review)
(2 Publications)
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA, WB
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(287 Publications)
Species: Mu
Applications: ELISA
CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
IL-2 [Unconjugated]
202-IL
Bioactivity IL-2 [Unconjugated]
(4 Reviews)
(369 Publications)
Species: Hu
Applications: BA
CD8 Antibody
(53-6.7) - BSA Free
NBP1-49045
Immunohistochemistry-Paraffin CD8 Antibody (53-6.7) - BSA Free
Immunocytochemistry/ Immunofluorescence CD8 Antibody (53-6.7) - BSA Free
Immunohistochemistry-Frozen CD8 Antibody (53-6.7) - BSA Free
(3 Reviews)
(26 Publications)
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free
(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
HLA DQ/DR/DP
Antibody (HLA-Pan/296 ...
NBP2-79843
Protein Array HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Western Blot HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
IL-17/IL-17A
[Biotin]
DY421
N/A IL-17/IL-17A [Biotin]
(185 Publications)
Species: Mu
Applications: ELISA
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(631 Publications)
Species: Mu
Applications: ELISA
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(445 Publications)
Species: Hu
Applications: BA
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(803 Publications)
Species: Hu
Applications: BA
CD3 Antibody
(17A2) [Unconjugated]
MAB4841
Immunohistochemistry CD3 Antibody (17A2) [Unconjugated]
Immunocytochemistry CD3 Antibody (17A2) [Unconjugated]
Immunohistochemistry CD3 Antibody (17A2) [Unconjugated]
(42 Publications)
Species: Mu
Applications: CompCytotox, CyTOF-ready, Flow, ICC, IHC, IP, Tstim
GITR/TNFRSF18
Antibody (110416) [Un ...
MAB689
Flow Cytometry GITR/TNFRSF18 Antibody (110416) [Unconjugated]
(11 Publications)
Species: Hu
Applications: Block, CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, WB
CD28 Antibody
(37407) [Unconjugated
MAB342
Agonist Activity CD28 Antibody (37407) [Unconjugated]
Immunocytochemistry CD28 Antibody (37407) [Unconjugated]
(1 Review)
(48 Publications)
Species: Hu
Applications: AgAct, ICC, WB
T-bet/TBX21
Antibody (525803) [Un ...
MAB5385
Western Blot T-bet/TBX21 Antibody (525803) [Unconjugated]
Intracellular Staining by Flow Cytometry T-bet/TBX21 Antibody (525803) [Unconjugated]
Intracellular Staining by Flow Cytometry T-bet/TBX21 Antibody (525803) [Unconjugated]
(4 Publications)
Species: Hu
Applications: ICC, ICFlow, WB
IL-7R alpha/CD127
Antibody (R34-34)
DDX0700P-100
Immunoprecipitation IL-7R alpha/CD127 Antibody (R34-34)
Flow Cytometry IL-7R alpha/CD127 Antibody (R34-34)
Flow Cytometry IL-7R alpha/CD127 Antibody (R34-34)
(40 Publications)
Species: Ca, Hu
Applications: B/N, Flow, IP
Doublecortin
Antibody
NBP1-72042
Western Blot Doublecortin Antibody
Immunocytochemistry/ Immunofluorescence Doublecortin Antibody
Immunocytochemistry/ Immunofluorescence Doublecortin Antibody
(3 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB

Publications for FoxP3 Antibody (FPK8214A) (0)

There are no publications for FoxP3 Antibody (FPK8214A).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for FoxP3 Antibody (FPK8214A) (0)

There are no reviews for FoxP3 Antibody (FPK8214A). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for FoxP3 Antibody (FPK8214A). (Showing 1 - 2 of 2 FAQ).

  1. Does FoxP3 antibodies comes in lyophilized form?
    • FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.
  2. What the theoretical molecular weight for FoxP3 antibodies?
    • The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.

Secondary Antibodies

 

Isotype Controls

Additional FoxP3 Products

Blogs on FoxP3.

Check out the latest blog posts on FoxP3.
Meeting Report: 2nd International Antibody Validation Meeting
Bio-Techne brands Novus Biologicals® and R&D Systems® were proud to support the 2nd International Antibody Validation Meeting held at Bath University, on the 15-16 September, 2016. Almost 100 participants from around the world, including funde...  Read full blog post.
FOXP3
Is has been established that the regulatory transcription factor FOXP3 (a member of the forkhead/winged-helix family of transcription factors) is imperative to immune system homeostasis through CD4+CD25+ regulatory T cell function.  Distinctively, ...  Read full blog post.
FOXP3 is a Master Regulator of T Regulatory (Treg) Cells
FOXP3, a member of forkhead/winged-helix family of transcription factors acts as a "master" regulator for the development and suppressive function of regulatory T cells (Tregs). Its constitutive expression is necessary for the suppressive function of ...  Read full blog post.
FOXP3: Master Regulatory Transcriptional Factor
FOXP3, a forkhead family transcription factor specially expressed in regulatory T (Treg) cells, controls the expression of many key immune-regulatory genes. Treg cells are a population of T lymphocytes that have critical roles in the immune system hom...  Read full blog post.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our FoxP3 Antibody (1054C) [Allophycocyanin] and receive a gift card or discount.


Bioinformatics

Gene Symbol FOXP3