![Western Blot: FOXO3 [p Ser425] Antibody [NBP3-16945] - Western blot analysis of extracts of K-562 cells, using FOXO3 antibody (NBP3-16945) at 1:1000 dilution.K-562 cells were treated by 10% FBS at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/FOXO3-[p-Ser425]-Antibody-Western-Blot-NBP3-16945-img0004.jpg "Western Blot: FOXO3 [p Ser425] Antibody [NBP3-16945] - Western blot analysis of extracts of K-562 cells, using FOXO3 antibody (NBP3-16945) at 1:1000 dilution.K-562 cells were treated by 10% FBS at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Immunogen | A synthetic phosphorylated peptide around S425 of human FOXO3 (NP_001446.1). LGSPT |
| Modification | p Ser425 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | FOXO3 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 50% glycerol, pH7.3 |
| Preservative | 0.01% Thimerosal |
| Purity | Affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for FOXO3 Antibody (NBP3-16945)Find related products by research area.
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| Gene Symbol | FOXO3 |
![Immunohistochemistry-Paraffin: FOXO3 [p Ser425] Antibody [NBP3-16945] - Immunohistochemistry of paraffin-embedded mouse heart using FOXO3 Rabbit pAb (NBP3-16945) at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/FOXO3-[p-Ser425]-Antibody-Immunohistochemistry-Paraffin-NBP3-16945-img0003.jpg "Immunohistochemistry-Paraffin: FOXO3 [p Ser425] Antibody [NBP3-16945] - Immunohistochemistry of paraffin-embedded mouse heart using FOXO3 Rabbit pAb (NBP3-16945) at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.")
![Immunohistochemistry-Paraffin: FOXO3 [p Ser425] Antibody [NBP3-16945] - Immunohistochemistry of paraffin-embedded rat kidney using FOXO3 Rabbit pAb (NBP3-16945) at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/FOXO3-[p-Ser425]-Antibody-Immunohistochemistry-Paraffin-NBP3-16945-img0001.jpg "Immunohistochemistry-Paraffin: FOXO3 [p Ser425] Antibody [NBP3-16945] - Immunohistochemistry of paraffin-embedded rat kidney using FOXO3 Rabbit pAb (NBP3-16945) at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.")
![Immunohistochemistry-Paraffin: FOXO3 [p Ser425] Antibody [NBP3-16945] - Immunohistochemistry of paraffin-embedded human lung cancer using FOXO3 Rabbit pAb (NBP3-16945) at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/FOXO3-[p-Ser425]-Antibody-Immunohistochemistry-Paraffin-NBP3-16945-img0002.jpg "Immunohistochemistry-Paraffin: FOXO3 [p Ser425] Antibody [NBP3-16945] - Immunohistochemistry of paraffin-embedded human lung cancer using FOXO3 Rabbit pAb (NBP3-16945) at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")