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FoxH1 Antibody [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF4248
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Catalog# & Formulation Size Price
Carrier Free
AF4248-SP
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FoxH1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human FoxH1
Ser164-Ser272
Accession # O75593
Specificity
Detects human FoxH1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human FoxB2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
FOXH1
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF4248 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FoxH1 Antibody [Unconjugated]

  • FAST1
  • Fast-1
  • FAST1forkhead box protein H1
  • FAST2
  • Fast-2
  • Forkhead activin signal transducer 1
  • Forkhead activin signal transducer 2
  • forkhead activin signal transducer-1
  • forkhead box H1
  • FoxH1
  • hFAST-1
  • TGF-beta/activin signal transducer

Background

FoxH1 (also known as FAST-1) is a 39 kDa, class 2, subfamily H member of the FOX (forkhead box) family of transcription factors. It is a nuclear protein that binds DNA promoter sites. It does not independently regulate transcription but interacts with SMAD2/4 to activate, and SMAD3/4 to inhibit gene transcription. Human FOXH1 is 365 amino acids (aa) in length. It contains an NLS (aa 22‑30), one FKH (fork head) DNA-binding domain (aa 32‑128) and a SMAD-interaction region (aa 273‑354). Over aa 164‑272, human FOXH1 is 55% identical to mouse FoxH1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FoxH1 Antibody (AF4248)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ChIP, Western Blot.


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ChIP
(1)
Western Blot
(1)
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(2)
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Product General Protocols

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Bioinformatics

Gene Symbol FOXH1
Uniprot