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FNBP1L Antibody - BSA Free

Images

 
Western Blot: FNBP1L Antibody [NBP1-76845] - Mouse brain tissue lysate with in (A) the absence and (B) the presence of blocking peptide with FNBP1L antibody at 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: FNBP1L Antibody [NBP1-76845] - Human Brain cells with TOCA-1 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: FNBP1L Antibody [NBP1-76845] - Human brain tissue with FNBP1L antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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FNBP1L Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human TOCA-1. The immunogen is located within the last 50 amino acids of TOCA-1. Amino Acid Squence: GWTRARRQNGEEGYVPT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FNBP1L
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1 ug/ml
Control Peptide
FNBP1L Peptide (NBP1-76845PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for FNBP1L Antibody - BSA Free

  • C1orf39
  • chromosome 1 open reading frame 39
  • FLJ20275
  • formin binding protein 1-like
  • formin-binding protein 1-like
  • toca-1
  • TOCA1Transducer of Cdc42-dependent actin assembly protein 1
  • transducer of Cdc42-dependent actin assembly 1

Background

Actin reorganization is important for the regulation of neuronal morphology. A protein involved in this process, the transducer of cdc42-dependent actin assembly 1 (TOCA-1) protein, a member of the evolutionarily conserved pombe CDC15 homology (PCH) protein family, is an essential component of the Cdc42 pathway. TOCA-1 binds both N-WASP and Cdc42 and is essential for Cdc42- and PIP2-induced actin polymerization, suggesting that TOCA-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex. Decreased expression of TOCA-1 significantly enhances neurite elongation in PC-12 cells; its overexpression in the same cells suppresses neurite elongation. It has been suggested that TOCA-1 negatively regulates axon branching by regulating membrane trafficking by regulating membrane trafficking through the F-BAR/EFC domain. Multiple isoforms of TOCA-1 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FNBP1L
Uniprot