![Western Blot: FKBP51/FKBP5 Antibody [NBP3-18673] - Figure 2. Western blot analysis using the antibody directed against mouse FKBP51/FKBP5 (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse FKBP51/FKBP5 , diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 51 kDa) is indicated on the right.](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-Western-Blot-NBP3-18673-img0001.jpg "Western Blot: FKBP51/FKBP5 Antibody [NBP3-18673] - Figure 2. Western blot analysis using the antibody directed against mouse FKBP51/FKBP5 (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse FKBP51/FKBP5 , diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 51 kDa) is indicated on the right.")
| Reactivity | MuSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Polyclonal antibody raised in rabbit against mouse FKBP51/FKBP5 (FK506 Binding Protein 51), using two KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein (1). |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | FKBP5 |
| Purity | Whole Antiserum |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | Whole antiserum |
| Preservative | 0.05% Sodium Azide |
| Purity | Whole Antiserum |
Secondary Antibodies |
Isotype Controls |
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![ELISA: FKBP51/FKBP5 Antibody [NBP3-18673] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse FKBP51/FKBP5 . The plates were coated with a mix of the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,400.](http://images.novusbio.com/fullsize/FKBP51-FKBP5 Antibody-ELISA-NBP3-18673-img0002.jpg "ELISA: FKBP51/FKBP5 Antibody [NBP3-18673] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse FKBP51/FKBP5 . The plates were coated with a mix of the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,400.")
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