DDX6 Antibody

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein. It may contribute to the cell proliferation and carcinogenesis.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.

Showing Publications 1 - 10 of 44. Show All 44 Publications. Collapse Publications.

Publications using NB200-191	Applications	Species
Heim A, Niedermeier ML, Stengel F, Mayer TU The translation regulator Zar1l controls timing of meiosis in Xenopus oocytes Development (Cambridge, England) 2022-11-01 [PMID: 36278895]
Venkataramanan S, Gadek M, Calviello L et al. DDX3X and DDX3Y are redundant in protein synthesis RNA 2021-12-01 [PMID: 34535544]
Paul S, Dansithong W, Figueroa KP et al. Staufen1 links RNA stress granules and autophagy in a model of neurodegeneration. Nat Commun 2018-09-07 [PMID: 30194296] (WB, Mouse)	WB	Mouse
Mehravar M, Kumar Y, Olshansky M et al. MOV10 facilitates messenger RNA decay in an N6-methyladenosine (m6A) dependent manner to maintain the mouse embryonic stem cells state bioRxiv 2021-08-12
Vladimir Majerciak, Tongqing Zhou, Michael J Kruhlak, Zhi-Ming Zheng RNA helicase DDX6 and scaffold protein GW182 in P-bodies promote biogenesis of stress granules Nucleic Acids Research 2023-09-22 [PMID: 37427791]
Sung CC, Poll B, Lin SH et al. Early Molecular Events Mediating Loss of Aquaporin-2 during Ureteral Obstruction in Rat Journal of the American Society of Nephrology : JASN 2022-08-02 [PMID: 35918145] (IHC-P, Rat) Details: Dilutions: 1:500	IHC-P	Rat
Hallacli E, Kayatekin C, Nazeen S et al. The Parkinson's disease protein alpha-synuclein is a modulator of processing bodies and mRNA stability Cell 2022-06-09 [PMID: 35688132] (ICC/IF, WB, Human)	ICC/IF, WB	Human
Shah A, Bhandari R IP6K1 upregulates the formation of processing bodies by influencing protein-protein interactions on the mRNA cap Journal of cell science 2021-11-29 [PMID: 34841428]
Shah A, Bhandari R IP6K1 upregulates the formation of processing bodies by promoting proteome remodeling on the mRNA cap bioRxiv 2020-07-14 [PMID: 34841428] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Somasekharan SP, Gleave M SARS-CoV-2 nucleocapsid protein interacts with immunoregulators and stress granules and phase separates to form liquid droplets FEBS letters 2021-11-15 [PMID: 34780058] (ICC/IF, Human)	ICC/IF	Human
Rink MR, Baptista MAP, Flomm FJ Et al. Concatemeric Broccoli reduces mRNA stability and induces aggregates PloS one 2021-08-04 [PMID: 34347781] (ICC/IF)	ICC/IF
Cougnoux A, Drummond RA, Fellmeth M, et al. GC content shapes mRNA storage and decay in human cells Elife 2019-12-19 [PMID: 31855182] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Sharma NR, Majerciak V, Kruhlak MJ et al. KSHV RNA-binding protein ORF57 inhibits P-body formation to promote viral multiplication by interaction with Ago2 and GW182 Nucleic Acids Res. 2019-08-10 [PMID: 31400113] (WB, Human)	WB	Human
Hubstenberger A, Courel M, Benard M, Souquere S et al. P-Body Purification Reveals the Condensation of Repressed mRNA Regulons Mol. Cell 2017-10-05 [PMID: 28965817] (ICC/IF, Human)	ICC/IF	Human
Ayache Jessica, Benard Marianne, Ernoult-Lange Michele et al. P-body assembly requires DDX6 repression complexes rather than decay or Ataxin2/2L complexes. Mol Biol Cell 2015-01-01 [PMID: 25995375] (WB, Human)	WB	Human
Somasekharan SP, El-Naggar A, Leprivier G et al. YB-1 regulates stress granule formation and tumor progression by translationally activating G3BP1. J. Cell Biol. 2015-03-30 [PMID: 25800057] (ICC/IF, Human)	ICC/IF	Human
de Vries S, Naarmann-de Vries IS, Urlaub H et al. Identification of DEAD-box RNA Helicase 6 (DDX6) as a Cellular Modulator of Vascular Endothelial Growth Factor Expression under Hypoxia. J Biol Chem. 2013-01-04 [PMID: 23293030] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Nihei Y, Ito D, Suzuki N. Roles of ataxin-2 in pathological cascades mediated by TAR DNA-binding Protein 43 (TDP-43) and Fused in Sarcoma (FUS) J Biol Chem 2012-10-09 [PMID: 23048034] (ICC/IF, Human)	ICC/IF	Human
Novotny I, Podolska K, Blazikova M et al. Nuclear LSm8 affects number of cytoplasmic processing bodies via controlling cellular distribution of Like-Sm proteins. Mol Biol Cell 2012-10-01 [PMID: 22875987]
Ernoult-Lange M, Baconnais S, Harper M et al. Multiple binding of repressed mRNAs by the P-body protein Rck/p54. RNA 2012-09-01 [PMID: 22836354]
Crist CG, Montarras D, Buckingham M et al. Muscle satellite cells are primed for myogenesis but maintain quiescence with sequestration of Myf5 mRNA targeted by microRNA-31 in mRNP granules. Cell Stem Cell 2012-07-01 [PMID: 22770245]
Cougot N, Cavalier A, Thomas D, Gillet R. The dual organization of P-bodies revealed by immunoelectron microscopy and electron tomography. J Mol Biol. 2012-04-03 [PMID: 22484175]
Marnef A, Weil D, Standart N et al. RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor. Mol Biol Cell 2012-01-01 [PMID: 22090346]
Saito K, Kondo E, Matsushita M et al. MicroRNA 130 family regulates the hypoxia response signal through the P-body protein DDX6. Nucleic Acids Res 2011-08-01 [PMID: 21486751]
Totaro A, Renzi F, La Fata G et al. The human Pat1b protein: a novel mRNA deadenylation factor identified by a new immunoprecipitation technique. Nucleic Acids Res 2011-01-01 [PMID: 20852261]
Marnef A, Maldonado M, Bugaut A et al. Distinct functions of maternal and somatic Pat1 protein paralogs. RNA 2010-11-01 [PMID: 20826699]
Jangra RK, Yi M, Lemon SM et al. DDX6 (Rck/p54) is required for efficient hepatitis C virus replication but not for internal ribosome entry site-directed translation. J Virol 2010-07-01 [PMID: 20392846]
Kemler I, Meehan A, Poeschla EM et al. Live-cell coimaging of the genomic RNAs and Gag proteins of two lentiviruses. J Virol 2010-07-01 [PMID: 20392841]
Savas JN, Ma B, Deinhardt K et al. A role for huntington disease protein in dendritic RNA granules. J Biol Chem 2010-04-01 [PMID: 20185826]
Emara MM, Ivanov P, Hickman T et al. Angiogenin-induced tRNA-derived stress-induced RNAs promote stress-induced stress granule assembly. J Biol Chem 2010-04-01 [PMID: 20129916]
Holzel M, Orban M, Hochstatter J et al. Defects in 18 S or 28 S rRNA processing activate the p53 pathway. J Biol Chem 2010-02-26 [PMID: 20056613] (Human)		Human
Souquere S, Mollet S, Kress M et al. Unravelling the ultrastructure of stress granules and associated P-bodies in human cells. J Cell Sci 2009-10-01 [PMID: 19812307]
Taddeo et al. PNAS 106 (29):12139-12144. 2009-01-01 [PMID: 19584246]
Otsuka et al. Mol.Cell.Biol. 29 (8):2155-2167. 2009-01-01 [PMID: 19223470]
Cougot et al. J.Neurosci. 28 (51):13793-13804. 2008-01-01 [PMID: 19091970]
Zhai et al. Mol.Cell.Biol. 28 (24):7414-7426. 2008-01-01 [PMID: 18936174]
Ernoult-Lange M, Wilczynska A, Harper M et al. Nucleocytoplasmic traffic of CPEB1 and accumulation in Crm1 nucleolar bodies. Mol Biol Cell. 2009-01-01 [PMID: 18923137]
Zheng et al. J.Cell Biol. 182 (1):89-101. 2008-01-01 [PMID: 18625844]
Morris AR, Mukherjee N, Keene JD et al. Ribonomic analysis of human Pum1 reveals cis-trans conservation across species despite evolution of diverse mRNA target sets. Mol Cell Biol 2008-06-01 [PMID: 18411299]
Marin et al. J.Virol. 82 (2):987-998. 2008-01-01 [PMID: 17977970]
Song MG, Kiledjian M. 3' Terminal oligo U-tract-mediated stimulation of decapping RNA. 2007-12-01 [PMID: 17942740]
Nonhoff, U, Ralser, M, Welzel, F, Piccini, I, Balzereit, D, Yaspo, M-L, Lehrach, H, Krobitsch, S. Ataxin-2 Interacts with the DEAD/H-Box RNA Helicase DDX6 Interferes with P-Bodies Stress Granules, . E06-12-1120. 2007-01-01 [PMID: 17392519]
Chu CY, Rana TM. Translation repression in human cells by microRNA-induced gene silencing requires RCK/p54. PLoS Biol 2006-07-01 [PMID: 16756390]
Wichroski MJ, Robb GB, Rana TM et al. Human retroviral host restriction factors APOBEC3G and APOBEC3F localize to mRNA processing bodies. PLoS Pathog 2006-05-01 [PMID: 16699599]
Show All 44 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Lifeng Liu	Immunofluorescence and microscopy	Hamster, Human, Mouse	08/22/2017	View Summary Application Other Sample Tested BHK (Baby Hamster Kidney fibroblasts),HOS human osteosarcoma cell line,MEFs,U2OS cells Species Hamster, Human, Mouse Lot A1 Comments Comments Cells were seeded on coverslips in 12-well plates overnight. Then, cells were fixed with 3.7% formaldehyde in PBS and blocked and permeabilized in PBS containing 5% horse serum (Sigma) and 0.3% Triton X-100 for 16 h at 4° C, followed by incubation with primary antibodies: rabbit-anti-DDX6 (1:1000) (NB200-191) for 1 h at room temperature. Coverslips were then washed with PBS and incubated with secondary antibodies (Alexa 488-conjugated donkey-anti-rabbit (1:200) and Hoechst 33258, followed by one more washing step and mounting on glass slides.

Array NB200-191

• DDX6 Antibodies
• DDX6 Lysate
• DDX6 Proteins