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Fibulin 2 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Fibulin 2 Antibody [NBP1-88115] - Analysis in human testis and liver tissues. Corresponding FBLN2 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Fibulin 2 Antibody [NBP1-88115] - Staining of human cell line BJ shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Fibulin 2 Antibody [NBP1-88115] - Staining of human urinary bladder shows strong positivity in connective tissue.
Immunohistochemistry-Paraffin: Fibulin 2 Antibody [NBP1-88115] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Fibulin 2 Antibody [NBP1-88115] - Staining of human skin shows strong positivity in connective tissue.
Immunohistochemistry-Paraffin: Fibulin 2 Antibody [NBP1-88115] - Staining of human testis shows strong membranous positivity in peritubular myoid cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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Fibulin 2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TLGSFHCYKALTCEPGYALKDGECEDVDECAMGTHTCQPGFLCQNTKGSFYCQARQRCMDGFLQDPEGNCVDINECTSLSEPCRPGFSCINTVGSYTCQRNPLICARGYHASDDGTKCVDVNE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FBLN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Fibulin 2 Protein (NBP1-88115PEP)
Publications
Read Publication using NBP1-88115.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (85%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Fibulin 2 Antibody

  • FBLN2
  • FIBL-2
  • Fibulin 2
  • Fibulin-2

Background

The Fibulin 2 gene encodes an extracellular matrix protein, which belongs to the fibulin family. This protein binds various extracellular ligands and calcium. It may play a role during organ development, in particular, during the differentiation of heart, skeletal

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Fibulin 2 Antibody (NBP1-88115)(1)

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol FBLN2