Fibulin 1 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725] - Analysis in human placenta and skeletal muscle tissues using NBP1-84725 antibody. Corresponding FBLN1 RNA-seq data are ...read more
Orthogonal Strategies: Western Blot: Fibulin 1 Antibody [NBP1-84725] - Analysis in human cell lines HeLa and SK-MEL-30 using Anti-FBLN1 antibody. Corresponding FBLN1 RNA-seq data are presented for the same cell ...read more
Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725] - Staining of human endometrium shows moderate positivity in extracellular matrix.
Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725] - Staining of human skin shows strong positivity in extracellular matrix.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Fibulin 1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: NTLGSYLCSCSVGFRLSVDGRSCEDINECSSSPCSQECANVYGSYQCYCRRGYQLSDVDGVTCEDIDECALPTGGHICSYRCINIPGSFQCSCPSSGYRLAPNGRNCQDIDECVTGIHNCSINETCFNIQGGFRCLAFECPEN
Predicted Species
Mouse (94%), Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FBLN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
IHC,HIER pH6 retrieval is recommended.
Control Peptide
Fibulin 1 Protein (NBP1-84725PEP)
Reviewed Applications
Read 1 Review rated 3
using
NBP1-84725 in the following applications:

Publications
Read Publications using
NBP1-84725 in the following applications:

  • B/N
    2 publications
  • 1 publication
  • WB
    1 publication

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24743550).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Fibulin 1 Antibody

  • FBLN
  • FBLN1
  • FIBL1
  • FIBL-1
  • Fibulin 1
  • fibulin-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IHC

Publications for Fibulin 1 Antibody (NBP1-84725)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: B/N, IHC-P, WB.


Filter By Application
B/N
(2)
IHC-P
(1)
WB
(1)
All Applications
Filter By Species
Human
(3)
All Species
Showing Publications 1 - 8 of 8.
Publications using NBP1-84725 Applications Species
Jabbari K, Cheng Q, Winkelmaier G Et al. CD36(+) Fibroblasts Secrete Protein Ligands That Growth-Suppress Triple-Negative Breast Cancer Cells While Elevating Adipogenic Markers for a Model of Cancer-Associated Fibroblast Int J Mol Sci 2022-11-11 [PMID: 36361532] (B/N, Human)

Details:
Citation using the FITC version of this antibody.
B/N Human
Jabbari K, Winkelmaier G, Andersen C et al. Protein Ligands in the Secretome of CD36+ Fibroblasts Induce Growth Suppression in a Subset of Breast Cancer Cell Lines Cancers 2021-09-08 [PMID: 34572749] (B/N) B/N
Okumura S, Oka S, Sasaki T et al. Spatiotemporal EP4-fibulin-1 expression is associated with vascular intimal hyperplasia bioRxiv 2023-11-14 [PMID: 34233723]
Bachmann J, Burte F, Pramana S et al. Affinity Proteomics Reveals Elevated Muscle Proteins in Plasma of Children with Cerebral Malaria. PLoS Pathog 2014-04-01 [PMID: 24743550]
Neiman M, Hedberg JJ, Donnes PR et al. Plasma profiling reveals human fibulin-1 as candidate marker for renal impairment. J Proteome Res 2011-11-01 [PMID: 21888404]
Kanda M, Nomoto S, Okamura Y et al. Promoter hypermethylation of fibulin 1 gene is associated with tumor progression in hepatocellular carcinoma. Mol Carcinog 2011-08-01 [PMID: 21268132]
Yan X, Guo Y, Chen J et al. The molecular interaction of ADAMTS-1 and fibulin-1 and its potential contribution to breast cancer biology JCMT 2019-05-05 (WB, Human) WB Human
Meunier M, Chapuis E, Lapierre L et al. Mannose-6-phosphate complex and improvement in biomechanical properties of the skin Journal of cosmetic dermatology 2021-02-12 [PMID: 33580613] (IHC-P, Human) IHC-P Human

Review for Fibulin 1 Antibody (NBP1-84725) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-84725:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Fibulin 1 NBP1-84725
Enlarge
3
reviewed by:
Verified Customer
IHC-P Human 04/11/2019
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman skin
SpeciesHuman
LotA71667

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Fibulin 1 Antibody (NBP1-84725) (0)

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Secondary Antibodies

 

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Verified Customer
04/11/2019
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol FBLN1