Neurocan Antibody

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Neurocan is a 220 kDa nervous tissue-specific chondroitin sulfate proteoglycan member of the aggrecan/versican proteoglycan family (1). Mouse Neurocan is synthesized as a 1268 amino acid (aa) precursor that contains a 22 aa signal sequence and a 1246 aa mature chain. The mature chain contains one Ig-like V-type domain (aa 37-157), two Link domains (aa 159-254 and 258-356), two EGF-like domains (aa 960-996 and 998-1034), one C-type lectin-like domain (aa 1036-1165), one Sushi domain (aa 1165-1224), and five potential sites for N-linked glycosylation. Mature mouse Neurocan is 90% and 66% aa identical to mature rat and human Neurocan, respectively. Neurocan binds with high affinity to the cell adhesion molecules (CAM) Ng-CAM and N-CAM to inhibit Neuronal adhesion and neurite growth (2-3). In the developing rat retina, the expression of Neurocan is regulated both temporally and spatially, which suggests that it may play a role in the differentiation of and neural network formation of the mammalian retina (1). Injury to the CNS leads to permanent loss of function due to the inability of severed nerve fibers to regenerate back to their targets (4). The lack of CNS repair is attributed in part to the extracellular matrix chondroitin sulfate proteoglycans, such as Neurocan, which are produced by activated glial cells post-injury (4).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: ELISA Development, IHC, IHC-Fr, IHC-P, Immunocytochemistry, Immunohistochemistry, Western Blot.

Showing Publications 1 - 10 of 21. Show All 21 Publications. Collapse Publications.

Publications using AF5800	Applications	Species
Otsuka, MY;Essel, LB;Sinha, A;Nickerson, G;Mejia, SM;Matthews, RT;Bouyain, S; The hyaluronan-binding activity of aggrecan is important, but not essential, for its specific insertion into perineuronal nets bioRxiv : the preprint server for biology 2024-11-25 [PMID: 39651196] (Immunocytochemistry, Mouse)	Immunocytochemistry	Mouse
Kuczynski-Noyau, L;Karmann, S;Alberton, P;Martinez-Corral, I;Nampoothiri, S;Sauvé, F;Lhomme, T;Quarta, C;Apte, SS;Bouret, S;Aszodi, A;Rasika, S;Ciofi, P;Dam, J;Prévot, V;Mattot, V; A plastic aggrecan barrier modulated by peripheral energy state gates metabolic signal access to arcuate neurons Nature communications 2024-08-07 [PMID: 39112471] (Immunohistochemistry, Mouse)	Immunohistochemistry	Mouse
D Irala, S Wang, K Sakers, L Nagendren, FP Ulloa-Seve, DS Bindu, C Eroglu Astrocyte-Secreted Neurocan Controls Inhibitory Synapse Formation and Function bioRxiv : the preprint server for biology, 2023-04-03;0(0):. 2023-04-03 [PMID: 37066164] (IHC, Western Blot, Mouse)	IHC, Western Blot	Mouse
L Höhn, W Hu beta ler, A Richter, KH Smalla, AM Birkl-Toeg, C Birkl, S Vielhaber, SL Leber, ED Gundelfing, J Haybaeck, S Schreiber, CI Seidenbech Extracellular Matrix Changes in Subcellular Brain Fractions and Cerebrospinal Fluid of Alzheimer's Disease Patients International Journal of Molecular Sciences, 2023-03-14;24(6):. 3/14/2023 [PMID: 36982604]
A Ritok, P Kiss, A Zaher, E Wolf, L Ducza, T Bacskai, C Matesz, B Gaal Distribution and postnatal development of chondroitin sulfate proteoglycans in the perineuronal nets of cholinergic motoneurons innervating extraocular muscles Scientific Reports, 2022-12-14;12(1):21606. 2022-12-14 [PMID: 36517521] (IHC, Mouse)	IHC	Mouse
W Hu beta ler, L Höhn, C Stolz, S Vielhaber, C Garz, FC Schmitt, ED Gundelfing, S Schreiber, CI Seidenbech Brevican and Neurocan Cleavage Products in the Cerebrospinal Fluid - Differential Occurrence in ALS, Epilepsy and Small Vessel Disease Frontiers in Cellular Neuroscience, 2022-04-11;16(0):838432. 2022-04-11 [PMID: 35480959] (ELISA Development, Human)	ELISA Development	Human
Sophie Schmidt, Max Holzer, Thomas Arendt, Mandy Sonntag, Markus Morawski Tau Protein Modulates Perineuronal Extracellular Matrix Expression in the TauP301L-acan Mouse Model Biomolecules 2022-03-26 [PMID: 35454094]
Josef Heusinger, Heika Hildebrandt, Robert‐Benjamin Illing Sensory deafferentation modulates and redistributes neurocan in the rat auditory brainstem Brain and Behavior 2019-08-01 [PMID: 31271523]
T Fontanil, Y Mohamedi, A Moncada-Pa, T Cobo, JA Vega, JL Cobo, O García-Suá, J Cobo, ÁJ Obaya, S Cal Neurocan is a New Substrate for the ADAMTS12 Metalloprotease: Potential Implications in Neuropathies Cell. Physiol. Biochem., 2019-01-01;52(5):1003-1016. 2019-01-01 [PMID: 30977985] (IHC-P, Western Blot, Mouse)	IHC-P, Western Blot	Mouse
H Ueno, S Suemitsu, S Murakami, N Kitamura, K Wani, Y Matsumoto, M Okamoto, T Ishihara Layer-specific expression of extracellular matrix molecules in the mouse somatosensory and piriform cortices IBRO Rep, 2018-11-28;6(0):1-17. 2018-11-28 [PMID: 30582064] (IHC-Fr, Mouse)	IHC-Fr	Mouse
M Sonntag, M Blosa, S Schmidt, K Reimann, K Blum, T Eckrich, G Seeger, D Hecker, B Schick, T Arendt, J Engel, M Morawski Synaptic coupling of inner ear sensory cells is controlled by brevican-based extracellular matrix baskets resembling perineuronal nets BMC Biol., 2018-09-26;16(1):99. 2018-09-26 [PMID: 30253762] (IHC, Western Blot, Mouse)	IHC, Western Blot	Mouse
J Chen, S Van Gulden, TL McGuire, AC Fleming, C Oka, JA Kessler, CY Peng BMP-responsive Protease HtrA1 is Differentially Expressed in Astrocytes and Regulates Astrocytic Development and Injury Response J. Neurosci., 2018-02-26;0(0):. 2018-02-26 [PMID: 29483282] (Western Blot, Mouse)	Western Blot	Mouse
H Ueno, S Suemitsu, M Okamoto, Y Matsumoto, T Ishihara Sensory experience-dependent formation of perineuronal nets and expression of Cat-315 immunoreactive components in the mouse somatosensory cortex Neuroscience, 2017-05-08;0(0):. 2017-05-08 [PMID: 28495333] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Protein O-Mannosylation in the Murine Brain: Occurrence of Mono-O-Mannosyl Glycans and Identification of New Substrates PLoS ONE, 2016-11-03;11(11):e0166119. 2016-11-03 [PMID: 27812179] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Kelly E. Carstens, Mary L. Phillips, Lucas Pozzo-Miller, Richard J. Weinberg, Serena M. Dudek Perineuronal Nets Suppress Plasticity of Excitatory Synapses on CA2 Pyramidal Neurons The Journal of Neuroscience 2016-06-08 [PMID: 27277807]
Shinji Miyata, Hiroshi Kitagawa Chondroitin 6-Sulfation Regulates Perineuronal Net Formation by Controlling the Stability of Aggrecan Neural Plasticity 2016-01-14 [PMID: 27057358]
Ebbers L, Satheesh S, Janz K, Ruttiger L, Blosa M, Hofmann F, Morawski M, Griesemer D, Knipper M, Friauf E, Nothwang H L-type Calcium Channel Cav1.2 Is Required for Maintenance of Auditory Brainstem Nuclei. J Biol Chem, 2015-08-04;290(39):23692-710. 2015-08-04 [PMID: 26242732] (IHC, Mouse)	IHC	Mouse
Robert Krencik, Kenton C. Hokanson, Aditi R. Narayan, Jill Dvornik, Gemma E. Rooney, Katherine A. Rauen et al. Dysregulation of astrocyte extracellular signaling in Costello syndrome Science Translational Medicine 2015-05-06 [PMID: 25947161]
Dwyer C, Katoh T, Tiemeyer M, Matthews R Neurons and glia modify receptor protein-tyrosine phosphatase zeta (RPTPzeta)/phosphacan with cell-specific O-mannosyl glycans in the developing brain. J Biol Chem, 2015-03-03;290(16):10256-73. 2015-03-03 [PMID: 25737452] (Western Blot, Mouse)	Western Blot	Mouse
Leon Garland Coleman, Wen Liu, Ipek Oguz, Martin Styner, Fulton T. Crews Adolescent binge ethanol treatment alters adult brain regional volumes, cortical extracellular matrix protein and behavioral flexibility Pharmacology Biochemistry and Behavior 2014-01-01 [PMID: 24275185]
Bekku Y, Oohashi T Neurocan contributes to the molecular heterogeneity of the perinodal ECM. Arch. Histol. Cytol., 2010-01-01;73(2):95-102. 2010-01-01 [PMID: 21566336] (IHC-Fr, Western Blot, Mouse)	IHC-Fr, Western Blot	Mouse
Show All 21 Publications. Collapse Publications.

Array AF5800

• Neurocan Antibodies
• Neurocan Protein
• Neurocan Proteins and Enzymes