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FHL1 Antibody (OTI3G7)

Images

 
Western Blot: FHL1 Antibody (3G7) [NBP2-02654] Analysis of extracts (35ug) from 9 different cell lines by using anti-FHL1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: FHL1 Antibody (3G7) [NBP2-02654] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FHL1.
Flow Cytometry: FHL1 Antibody (3G7) [NBP2-02654] - Analysis of Jurkat cells, using anti-FHL1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: FHL1 Antibody (3G7) [NBP2-02654] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Flow Cytometry: FHL1 Antibody (3G7) [NBP2-02654] - Analysis of Hela cells, using anti-FHL1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
OTI3G7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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FHL1 Antibody (OTI3G7) Summary

Immunogen
Full length human recombinant protein of human FHL1 (NP_001440) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
FHL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500-2000
Theoretical MW
31.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for FHL1 Antibody (OTI3G7)

  • bA535K18.1
  • FHL1
  • four and a half LIM domains 1
  • HLH1
  • HPLH1
  • KYOT
  • KYO-T
  • LIM protein SLIMMER
  • skeletal muscle LIM-protein1
  • SLIM1
  • SLIM1Isl-1 and Mec-3 domains 1
  • SLIMMER
  • XMPMA

Background

This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FHL1
Entrez