FBXW11 Antibody

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Western Blot: FBXW11 Antibody [NBP1-78060] - This antibody was used at a 1:200 dilution incubated in 5% BLOTTO overnight at 4C. Detection occurred using HRP conjugated Goat-anti-Rabbit IgG diluted 1:20,000 in blocking ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

FBXW11 Antibody Summary

Description
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Immunogen
This affinity purified FBXW11 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminal of human FBXW11 protein. (Uniprot: Q9UKB1)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FBXW11
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:10000-1:100000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Western Blot 1:200-1:1000
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blotting.
Control Peptide
FBXW11 Protein (NBP1-96762)

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with FBXW11 from human, mouse, dog, bovine, chimpanzee, chicken and horse based on a 100% homology with the immunizing sequence. Cross-reactivity with FBXW11 from other sources has not been determined.

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for FBXW11 Antibody

  • beta-transducin repeat-containing protein 2
  • BTRC2F-box/WD repeat-containing protein 1B
  • BTRCP2FBW1B
  • F-box and WD repeat domain containing 11
  • F-box and WD-40 domain protein 11
  • F-box and WD-40 domain protein 1B
  • F-box protein Fbw1b
  • F-box/WD repeat-containing protein 11
  • Fbw11
  • FBXW1BFbw1b
  • Homologous to Slimb protein
  • Hos
  • KIAA0696F-box and WD repeats protein beta-TrCP2

Background

FBXW11 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class and, in addition to an F-box, contains multiple WD40 repeats.This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class and, in addition to an F-box, contains multiple WD40 repeats. This gene contains at least 14 exons, and its alternative splicing generates 3 transcript variants diverging at the presence/absence of two alternate exons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol FBXW11
Entrez
Uniprot