Novus Biologicals products are now on bio-techne.com

Skp1 Antibody

Images

 
Western Blot: Skp1 Antibody [NBP2-20380] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SKP1 antibody diluted at 1:500. ...read more
Immunohistochemistry-Paraffin: Skp1 Antibody [NBP2-20380] - Paraffin-embedded mouse middle brain. SKP1 antibody dilution: 1:500.
Western Blot: Skp1 Antibody [NBP2-20380] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE gel, diluted at 1:1000.
Western Blot: Skp1 Antibody [NBP2-20380] - Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE gel, diluted at 1:3000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, Gp, RM, Sh, Xp, ZeSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Skp1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Skp1. The exact sequence is proprietary.
Predicted Species
Rat (100%), Chicken (100%), Canine (100%), Sheep (100%), Xenopus (100%), Bovine (100%), Guinea Pig (100%), Zebrafish (99%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SKP1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus Laevis (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Skp1 Antibody

  • Cyclin-A/CDK2-associated protein p19
  • EMC19p19skp1
  • MGC34403
  • OCP2
  • OCP2OCP-2
  • OCP-IIS-phase kinase-associated protein 1A (p19A)
  • Organ of Corti protein 2
  • Organ of Corti protein II
  • p19a
  • p19Acyclin A/CDK2-associated p19
  • p19Skp1
  • RNA polymerase II elongation factor-like protein OCP2
  • RNA polymerase II elongation factor-like protein
  • SKP1
  • SKP1A
  • SKP1Acyclin A/CDK2-associated protein p19
  • S-phase kinase-associated protein 1
  • TCEB1LSIII
  • Transcription elongation factor B

Background

This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

255-SC
Species: Hu
Applications: BA
NBP3-16092
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP1-31302
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
NBP1-89150
Species: Hu, Rt
Applications: IHC, IHC-P, WB
H00011026-M01
Species: Hu
Applications: ELISA, WB
H00143384-P01
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
NBP3-21835
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IP, WB
NBP2-31361
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-81988
Species: Hu
Applications: IHC, IHC-P
NBP2-21037
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-49142
Species: Hu
Applications: IHC, IHC-P, KD, WB
NBP2-61712
Species: Hu
Applications: ELISA, Flow, IHC, IHC-P, WB
NBP1-59631
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-44520
Species: Ca(-), Hu, Rt(-)
Applications: Flow-IC, Flow, ICC/IF, IF, IHC, IHC-P, MI, WB
NBP1-87581
Species: Hu
Applications: IHC, IHC-P, WB
H00026232-B01P
Species: Hu, Rt
Applications: WB
NB100-91662
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP2-29463
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-87104
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-20380
Species: Hu, Mu, Rt, Bv, Ca, Ch, Gp, RM, Sh, Xp, Ze
Applications: WB, IHC
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Skp1 Antibody (NBP2-20380) (0)

There are no publications for Skp1 Antibody (NBP2-20380).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Skp1 Antibody (NBP2-20380) (0)

There are no reviews for Skp1 Antibody (NBP2-20380). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Skp1 Antibody (NBP2-20380) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Skp1 Products

Research Areas for Skp1 Antibody (NBP2-20380)

Find related products by research area.

Blogs on Skp1

There are no specific blogs for Skp1, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Skp1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol SKP1
Uniprot